FastQCFastQC Report
Wed 25 May 2016
SRR2088422_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088422_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423120
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53181.2568538476082434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50021.1821705426356588No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45431.0736906787672527No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG23050.5447627150690112No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16000.37814331631688414No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA13740.3247305728871242No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT12620.29826054074494235No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA12550.29660616373605597No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT12350.29187937228209493No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA10760.25430138022310456No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT10290.24319342030629607No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG8710.2058517678200038No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT8620.20372471166572131No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA8060.19048969559463036No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA7520.17772735866893552No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA7390.17465494422386083No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT7220.17063717148799395No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6850.161892607298166No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA5840.13802231045566268No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC5280.12478729438457174No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC5010.11840612592172434No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4790.11320665532236716No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG4700.1110795991680847No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA4520.10682548685951976No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT4450.10517110985063338No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG4420.10446209113253924No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGACCG200.001840621837.09
CTATCCA250.00549246729.64
AGACCTA250.00549246729.65
GGTATCA12600.029.071431
CGTTTAG358.8600954E-426.42857226
AAATGCG358.8600954E-426.42857234
AATGCGT451.3212822E-424.66666835
TGCGTTA400.001929181523.12500237
ATTTTCG555.135716E-420.18181815
ATGCGTT555.135716E-420.18181836
AACAGGC555.135716E-420.1818187
ATCTGCG555.135716E-420.18181836
TCTCTAT759.243098E-619.7333348
ATCTCTA902.1456817E-618.57
GTTTAGA609.2236703E-418.51
TGCGTTT609.2236703E-418.524
TAATCTC852.7165363E-517.4117645
GTATCAA21400.017.1168232
ATTAATC1352.2077074E-816.4444453
TCAGGAC803.3762498E-416.18753
Produced by FastQC (version 0.11.5)