##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088422_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	423120
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27283749290981	31.0	31.0	34.0	30.0	34.0
2	31.516581584420496	31.0	31.0	34.0	30.0	34.0
3	31.6075770467007	31.0	31.0	34.0	30.0	34.0
4	35.40873511060692	37.0	35.0	37.0	33.0	37.0
5	35.23500189071658	37.0	35.0	37.0	33.0	37.0
6	35.27239317451314	37.0	35.0	37.0	32.0	37.0
7	35.13431414256003	37.0	35.0	37.0	32.0	37.0
8	35.162811968235964	37.0	35.0	37.0	32.0	37.0
9	36.6467503308754	39.0	35.0	39.0	32.0	39.0
10	36.54684486670448	39.0	35.0	39.0	32.0	39.0
11	36.65799536774438	39.0	35.0	39.0	32.0	39.0
12	36.515241539043295	38.0	35.0	39.0	32.0	39.0
13	36.53396435999244	39.0	35.0	39.0	32.0	39.0
14	37.508468046889774	39.0	36.0	41.0	32.0	41.0
15	37.50398941198714	39.0	36.0	41.0	32.0	41.0
16	37.4591534316506	39.0	36.0	41.0	32.0	41.0
17	37.53607014558518	39.0	36.0	41.0	32.0	41.0
18	37.529284836453016	39.0	36.0	41.0	32.0	41.0
19	37.61581820760068	39.0	37.0	41.0	32.0	41.0
20	37.56656504065041	39.0	36.0	41.0	32.0	41.0
21	37.50038996029495	39.0	36.0	41.0	32.0	41.0
22	37.412329835507656	39.0	36.0	41.0	32.0	41.0
23	37.28053743618832	39.0	36.0	41.0	31.0	41.0
24	37.22352524106636	39.0	36.0	41.0	31.0	41.0
25	37.169720173945926	39.0	36.0	41.0	31.0	41.0
26	36.9175458498771	39.0	35.0	40.0	31.0	41.0
27	36.76043439213462	39.0	35.0	40.0	30.0	41.0
28	36.66163499716392	39.0	35.0	40.0	30.0	41.0
29	36.502197958026095	38.0	35.0	40.0	30.0	41.0
30	36.33450557761392	38.0	35.0	40.0	30.0	41.0
31	36.062058044999056	38.0	35.0	40.0	30.0	41.0
32	35.9037932501418	38.0	35.0	40.0	29.0	41.0
33	35.92955899035734	38.0	35.0	40.0	30.0	41.0
34	35.854395916052184	38.0	35.0	40.0	29.0	41.0
35	35.77015031196824	38.0	35.0	40.0	29.0	41.0
36	35.69502032520325	38.0	35.0	40.0	28.0	41.0
37	35.57919739081112	38.0	34.0	40.0	27.0	41.0
38	35.44538901493666	38.0	34.0	40.0	27.0	41.0
39	35.37436424654944	38.0	34.0	40.0	26.0	41.0
40	35.19322178105502	38.0	34.0	40.0	25.0	41.0
41	35.026774910190966	38.0	34.0	40.0	24.0	41.0
42	34.86015314804311	38.0	34.0	40.0	24.0	41.0
43	34.508947816222346	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	8.0
17	11.0
18	32.0
19	58.0
20	134.0
21	269.0
22	495.0
23	880.0
24	1466.0
25	2337.0
26	3576.0
27	5155.0
28	7304.0
29	9886.0
30	12653.0
31	15837.0
32	18723.0
33	23024.0
34	28464.0
35	34182.0
36	42756.0
37	58101.0
38	74341.0
39	83427.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.813244469653995	22.799205899035734	14.140669313669882	22.246880317640386
2	17.81811306485158	23.04972584609567	36.881026659103796	22.25113442994895
3	19.67857818113065	26.230383815466062	32.09680468897712	21.994233314426168
4	12.988041217621479	16.09354320287389	35.769757988277554	35.14865759122707
5	11.655795046322556	40.31669502741539	34.40678767252789	13.620722253734167
6	31.073690678767253	36.689118926072986	15.778502552467385	16.45868784269238
7	24.468708640574775	35.07917375685385	22.091605218377765	18.36051238419361
8	27.1847230100208	33.932690489695595	20.573359803365477	18.30922669691813
9	25.405322367177156	14.096001134429951	23.717857818113064	36.78081868027982
10	16.460578559273962	27.478493098884478	33.79655889582152	22.26436944602004
11	31.49437511816979	22.400973719039516	25.081300813008127	21.023350349782568
12	20.229013045944413	27.849782567593117	31.64657780298733	20.274626583475136
13	30.689875212705616	21.189969748534697	28.661608999810927	19.45854603894876
14	20.807099640763848	22.837256570240122	28.675789374172812	27.679854414823218
15	22.83512951408584	29.391898279447908	26.89662507090187	20.87634713556438
16	21.66524862923048	28.633957269805254	27.25586122140291	22.444932879561357
17	19.621147664965022	27.427916430327095	28.064142560030252	24.88679334467763
18	20.639298544148232	26.546133484590662	32.095386651540935	20.719181319720175
19	19.92957080733598	25.436046511627907	33.22461712989223	21.409765551143884
20	23.83815466061637	24.39213461902061	32.18708640574778	19.582624314615238
21	23.483408961996595	25.30251465305351	30.895017961807525	20.319058423142373
22	21.95547362450369	24.987237663074303	31.71369824163358	21.343590470788428
23	20.810881073927018	26.555114388353186	32.818349404424275	19.815655133295518
24	19.656125921724332	26.013187748156554	31.419219134051808	22.91146719606731
25	20.727689544337306	26.687464549064092	32.42366231801853	20.161183588580073
26	22.01314048024201	25.952921157118546	31.202968425033088	20.83096993760635
27	20.806390622045758	27.205284552845526	30.75865002836075	21.229674796747968
28	20.564142560030252	26.542588391000187	31.03516732841747	21.85810172055209
29	19.59231423709586	26.72433352240499	31.795944412932503	21.887407827566648
30	19.234968803176404	26.781291359425225	33.43779542446587	20.545944412932503
31	20.512620533182076	26.176971072036302	32.50519947059936	20.805208924182264
32	21.269143505388545	26.428436377387033	30.698856116468143	21.603564000756286
33	22.05426356589147	26.404802420117225	30.447390811117415	21.09354320287389
34	20.77802987332199	28.412270750614482	30.53010966156173	20.279589714501796
35	19.459018718094157	29.369446020041597	30.74517867271696	20.42635658914729
36	20.22570429192664	26.165390432974096	32.39317451314048	21.21573076195878
37	20.33512951408584	26.266071090943466	31.784363773870293	21.614435621100398
38	20.52420117224428	25.85271317829457	32.43831537152581	21.18477027793534
39	21.27197958026092	25.511911514463982	32.333380601247875	20.882728304027225
40	19.755624881830215	26.25118169786349	31.64279636982416	22.350397050482133
41	20.489931934203064	25.861694082057102	31.425600302514656	22.222773681225185
42	18.375638116846286	27.52008886367933	31.78554547173379	22.318727547740593
43	18.932926829268293	27.267205520892418	31.344772168651918	22.45509548118737
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	48.0
1	58.0
2	68.0
3	211.0
4	354.0
5	354.0
6	586.0
7	818.0
8	895.0
9	972.0
10	1439.0
11	1906.0
12	1906.0
13	3299.5
14	4693.0
15	7752.5
16	10812.0
17	10720.0
18	10628.0
19	10628.0
20	11429.0
21	12230.0
22	9992.0
23	7754.0
24	8516.5
25	9279.0
26	9279.0
27	10019.0
28	10759.0
29	11437.5
30	12116.0
31	12841.5
32	13567.0
33	13567.0
34	14647.5
35	15728.0
36	17196.5
37	18665.0
38	19521.0
39	20377.0
40	20377.0
41	21570.5
42	22764.0
43	25614.5
44	28465.0
45	34727.0
46	40989.0
47	40989.0
48	40996.5
49	41004.0
50	37326.5
51	33649.0
52	31414.0
53	29179.0
54	29179.0
55	25447.0
56	21715.0
57	18338.5
58	14962.0
59	13231.0
60	11500.0
61	11500.0
62	10196.0
63	8892.0
64	7724.0
65	6556.0
66	5516.5
67	4477.0
68	4477.0
69	3809.0
70	3141.0
71	2646.5
72	2152.0
73	1724.5
74	1297.0
75	1297.0
76	978.0
77	659.0
78	519.5
79	380.0
80	321.0
81	262.0
82	262.0
83	187.5
84	113.0
85	96.0
86	79.0
87	75.0
88	71.0
89	71.0
90	51.5
91	32.0
92	19.0
93	6.0
94	3.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	423120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.72959562778709
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.00719875539883	51.371752027386854
2	8.078786094778382	9.650852532090038
3	2.156144087681735	3.863568434174218
4	1.0008395732086708	2.391189719841637
5	0.580084875486761	1.7324117521309732
6	0.4178300426384908	1.4974091692762854
7	0.30255152495367416	1.2649896169437302
8	0.19906772320037097	0.9512187687441929
9	0.1667630408359266	0.8964620095311038
>10	0.9723443245462097	10.61689140807322
>50	0.06833245856017053	2.7647214154076916
>100	0.041714582258975	5.156178150683244
>500	0.003972817357997619	1.6729281587885714
>1k	0.003575535622197858	3.720544766190923
>5k	7.945634715995238E-4	2.448882070737313
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5318	1.2568538476082434	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5002	1.1821705426356588	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4543	1.0736906787672527	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2305	0.5447627150690112	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1600	0.37814331631688414	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1374	0.3247305728871242	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1262	0.29826054074494235	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1255	0.29660616373605597	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1235	0.29187937228209493	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1076	0.25430138022310456	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1029	0.24319342030629607	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	871	0.2058517678200038	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	862	0.20372471166572131	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	806	0.19048969559463036	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	752	0.17772735866893552	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	739	0.17465494422386083	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	722	0.17063717148799395	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	685	0.161892607298166	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	584	0.13802231045566268	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	528	0.12478729438457174	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	501	0.11840612592172434	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	479	0.11320665532236716	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	470	0.1110795991680847	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	452	0.10682548685951976	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	445	0.10517110985063338	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	442	0.10446209113253924	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.3633957269805257E-4	0.0
10	0.0	0.0	0.0	9.453582907922103E-4	0.0
11	0.0	0.0	0.0	0.002127056154282473	0.0
12	0.0	0.0	0.0	0.0028360748723766306	0.0
13	0.0	0.0	0.0	0.0033087540177727357	0.0
14	0.0	0.0	0.0	0.003781433163168841	0.0
15	0.0	0.0	0.0	0.005672149744753261	0.0
16	0.0	0.0	0.0	0.006853847608243525	0.0
17	0.0	0.0	0.0	0.011344299489506523	0.0
18	0.0	0.0	0.0	0.01205331820760068	0.0
19	0.0	0.0	0.0	0.013944034789185102	0.0
20	0.0	0.0	0.0	0.017252788806957835	0.0
21	0.0	0.0	0.0	0.024579315560597465	0.0
22	0.0	0.0	0.0	0.04159576479485725	0.0
23	0.0	0.0	0.0	0.05790319531102288	0.0
24	0.0	0.0	0.0	0.08626394403478918	0.0
25	0.0	0.0	0.0	0.09500850822461714	0.0
26	0.0	0.0	0.0	0.1051711098506334	0.0
27	0.0	0.0	0.0	0.17961807525051995	0.0
28	0.0	0.0	0.0	0.2734448856116468	0.0
29	0.0	0.0	0.0	0.39137833238797504	0.0
30	0.0	0.0	0.0	0.6700226885989791	0.0
31	0.0	0.0	0.0	1.0628190584231423	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTGACCG	20	0.0018406218	37.0	9
CTATCCA	25	0.005492467	29.6	4
AGACCTA	25	0.005492467	29.6	5
GGTATCA	1260	0.0	29.07143	1
CGTTTAG	35	8.8600954E-4	26.428572	26
AAATGCG	35	8.8600954E-4	26.428572	34
AATGCGT	45	1.3212822E-4	24.666668	35
TGCGTTA	40	0.0019291815	23.125002	37
ATTTTCG	55	5.135716E-4	20.181818	15
ATGCGTT	55	5.135716E-4	20.181818	36
AACAGGC	55	5.135716E-4	20.181818	7
ATCTGCG	55	5.135716E-4	20.181818	36
TCTCTAT	75	9.243098E-6	19.733334	8
ATCTCTA	90	2.1456817E-6	18.5	7
GTTTAGA	60	9.2236703E-4	18.5	1
TGCGTTT	60	9.2236703E-4	18.5	24
TAATCTC	85	2.7165363E-5	17.411764	5
GTATCAA	2140	0.0	17.116823	2
ATTAATC	135	2.2077074E-8	16.444445	3
TCAGGAC	80	3.3762498E-4	16.1875	3
>>END_MODULE