FastQCFastQC Report
Wed 25 May 2016
SRR2088421_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088421_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396936
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107200.7673937818196396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104590.7487100339600382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT87880.6290910965140851No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38890.2783950016321435No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG22510.161138377133956No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15170.10859481035637997No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT14280.10222372392149676No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31250.023.79841
CCCGTAT656.903492E-519.9230771
TAGCCCG801.6170763E-518.55
CCGTATA902.1528904E-618.52
CGGGCGA701.2194361E-418.528
ATGACGG953.6075107E-617.52631620
TGCGGTC852.723795E-517.41176412
GTCCTAT1951.8189894E-1216.1282061
GTACGGT700.002592868915.85714211
CGGACCA2150.015.4883729
TTGCGTG1201.9361287E-615.4166667
AAGACCG1101.4522342E-515.1363645
GTATCAA49100.015.0712832
GTAGACC750.00410499514.83
ACGGACC2151.2732926E-1114.6279078
CGCAAGA2151.2732926E-1114.6279072
CGTATAC1152.2104074E-514.4782613
ACGCCTA908.275586E-414.3888896
GTATACC908.275586E-414.3888894
TATACCG908.275586E-414.3888895
Produced by FastQC (version 0.11.5)