##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088421_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1396936
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.212213014769468	31.0	31.0	34.0	30.0	34.0
2	31.461238023789207	31.0	31.0	34.0	30.0	34.0
3	31.555167165854414	31.0	31.0	34.0	30.0	34.0
4	35.358860391599904	37.0	35.0	37.0	33.0	37.0
5	35.14547767399509	37.0	35.0	37.0	32.0	37.0
6	35.18222667323342	37.0	35.0	37.0	32.0	37.0
7	35.06775256704674	37.0	35.0	37.0	32.0	37.0
8	35.078292062055816	37.0	35.0	37.0	32.0	37.0
9	36.59440804732643	39.0	35.0	39.0	32.0	39.0
10	36.43363403906836	38.0	35.0	39.0	32.0	39.0
11	36.558324790827925	38.0	35.0	39.0	32.0	39.0
12	36.42456920002061	38.0	35.0	39.0	32.0	39.0
13	36.46905155282705	38.0	35.0	39.0	32.0	39.0
14	37.471564194780576	39.0	36.0	41.0	32.0	41.0
15	37.466523162120524	39.0	36.0	41.0	32.0	41.0
16	37.42878485485377	39.0	36.0	41.0	32.0	41.0
17	37.43899505775497	39.0	36.0	41.0	32.0	41.0
18	37.408601396198534	39.0	36.0	41.0	32.0	41.0
19	37.47699679870803	39.0	36.0	41.0	32.0	41.0
20	37.434894655159574	39.0	36.0	41.0	32.0	41.0
21	37.385606785135465	39.0	36.0	41.0	31.0	41.0
22	37.27722315123957	39.0	36.0	41.0	31.0	41.0
23	37.18468992137077	39.0	36.0	41.0	31.0	41.0
24	37.11103085610221	39.0	36.0	41.0	31.0	41.0
25	37.04766288505701	39.0	36.0	40.0	31.0	41.0
26	36.812377947164364	39.0	35.0	40.0	30.0	41.0
27	36.67155689308601	39.0	35.0	40.0	30.0	41.0
28	36.58180689738113	39.0	35.0	40.0	30.0	41.0
29	36.44384567367438	38.0	35.0	40.0	30.0	41.0
30	36.282738078193994	38.0	35.0	40.0	30.0	41.0
31	36.09001700865323	38.0	35.0	40.0	29.0	41.0
32	35.92799526964728	38.0	34.0	40.0	29.0	41.0
33	36.036424002244914	38.0	35.0	40.0	30.0	41.0
34	36.01189961458506	38.0	35.0	40.0	29.0	41.0
35	35.9763224657393	38.0	35.0	40.0	29.0	41.0
36	35.905546138119426	38.0	35.0	40.0	29.0	41.0
37	35.803932320449896	38.0	35.0	40.0	28.0	41.0
38	35.698938247707844	38.0	34.0	40.0	27.0	41.0
39	35.67056114238591	38.0	34.0	40.0	27.0	41.0
40	35.560939799675864	38.0	34.0	40.0	27.0	41.0
41	35.45096482587606	38.0	34.0	40.0	26.0	41.0
42	35.35958984520408	38.0	34.0	40.0	26.0	41.0
43	35.01620618267408	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	1.0
14	2.0
15	10.0
16	12.0
17	37.0
18	106.0
19	234.0
20	453.0
21	933.0
22	1688.0
23	2930.0
24	4849.0
25	7734.0
26	11744.0
27	17046.0
28	23723.0
29	32186.0
30	40711.0
31	51053.0
32	62513.0
33	76208.0
34	92831.0
35	112836.0
36	140946.0
37	186108.0
38	254425.0
39	275616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.54280224720388	22.552214274669705	13.36102727684017	23.543956201286242
2	17.994883086984657	24.049777513071465	34.970320759147164	22.985018640796714
3	19.567109731583983	26.344012896796993	31.80532250582704	22.28355486579199
4	13.730765045785919	17.324487306505095	36.81084888642	32.13389876128899
5	12.583396805580213	39.40230619011895	34.735807510150785	13.278489494150053
6	30.756885068464122	38.210555100591584	16.412562923426698	14.619996907517596
7	24.981029911177032	34.079585607357814	23.218601281662153	17.720783199802998
8	26.15109067272946	34.42111879141206	21.176918627625028	18.25087190823345
9	25.78328570528643	14.163927338117135	22.648782764564736	37.40400419203171
10	15.781539025409897	27.993050504819116	34.768378794733614	21.457031675037367
11	32.53513403620495	23.35869359798874	23.9896459107647	20.116526455041605
12	19.65537433354141	27.73319608056489	32.01685689251333	20.59457269338037
13	28.95744686943425	21.852898056890222	27.698477238756823	21.491177834918705
14	20.793436492437735	23.088387728571675	28.356918284015876	27.761257494974718
15	23.19583717507459	29.880395379602216	25.28913278775835	21.634634657564842
16	21.876163260163672	28.744623948412812	27.050058127215564	22.329154664207955
17	20.3571244495095	28.280536832038116	28.447831539884433	22.914507178567952
18	20.726361121769358	27.418221020862806	30.512421470990798	21.34299638637704
19	20.95872681354049	27.038962414885148	31.37359191831265	20.62871885326171
20	22.569824243916685	26.36799395247885	30.49194809211016	20.5702337114943
21	22.49644937205427	26.93867149246637	29.786976640304207	20.777902495175155
22	21.900359071568058	26.81976840742883	30.39716923323617	20.88270328776694
23	21.260888115131973	27.285430399102033	30.42823722776133	21.02544425800466
24	20.802957329469642	27.46088582440427	30.05449068532846	21.68166616079763
25	21.433122204596344	27.61729957564269	30.255859967815272	20.693718251945686
26	21.58967912631645	27.51743816466896	29.72240675306528	21.170475955949307
27	21.308134374087288	27.861548417393493	29.670077942010227	21.160239266508988
28	20.95299999427318	27.990759777112196	29.82541791463603	21.230822313978592
29	20.483329229112858	27.712507945961733	30.472906418046353	21.331256406879056
30	20.576175286484133	28.10314860523317	30.671913387585402	20.6487627206973
31	21.089513048557702	27.963342629869942	30.084126975036796	20.86301734653556
32	21.074265392258486	27.956184105785802	29.747604757841444	21.22194574411426
33	21.285155511777205	27.88452727970358	29.69434533865546	21.13597186986376
34	21.17835033244186	28.213962558055634	29.709807750677196	20.897879358825314
35	20.89293997720726	28.378966538195023	29.941099663835708	20.78699382076201
36	20.83187776676956	27.68351592342097	30.247484494636833	21.237121815172635
37	20.895087534432502	27.30175183401387	30.226295263347787	21.576865368205844
38	20.815699502339406	27.1552884312524	30.584078297073024	21.444933769335172
39	21.112563496108628	26.917840187381525	30.6603165785691	21.309279737940752
40	20.44925465447236	26.903308383490725	30.84092614121191	21.806510820825007
41	20.437514674974373	26.59406014305595	30.91322723446171	22.055197947507974
42	19.674988689531947	27.296311355709925	30.884521552884316	22.144178401873816
43	19.736408826173854	26.691416070600226	30.966916165092744	22.605258938133172
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	233.0
1	280.0
2	327.0
3	758.5
4	1190.0
5	1190.0
6	1879.5
7	2569.0
8	2708.5
9	2848.0
10	4258.0
11	5668.0
12	5668.0
13	9263.5
14	12859.0
15	19244.0
16	25629.0
17	25319.0
18	25009.0
19	25009.0
20	28313.0
21	31617.0
22	28804.0
23	25991.0
24	29944.5
25	33898.0
26	33898.0
27	37507.0
28	41116.0
29	44127.5
30	47139.0
31	51804.5
32	56470.0
33	56470.0
34	61362.0
35	66254.0
36	71665.5
37	77077.0
38	80014.0
39	82951.0
40	82951.0
41	85974.5
42	88998.0
43	92350.0
44	95702.0
45	101887.0
46	108072.0
47	108072.0
48	107964.0
49	107856.0
50	100064.5
51	92273.0
52	87172.5
53	82072.0
54	82072.0
55	75885.5
56	69699.0
57	63992.0
58	58285.0
59	52427.0
60	46569.0
61	46569.0
62	41098.0
63	35627.0
64	30792.5
65	25958.0
66	21526.0
67	17094.0
68	17094.0
69	14492.5
70	11891.0
71	9957.5
72	8024.0
73	6190.5
74	4357.0
75	4357.0
76	3276.0
77	2195.0
78	1790.0
79	1385.0
80	1112.0
81	839.0
82	839.0
83	656.5
84	474.0
85	375.5
86	277.0
87	254.0
88	231.0
89	231.0
90	196.0
91	161.0
92	97.0
93	33.0
94	23.5
95	14.0
96	14.0
97	9.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1396936.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.27148345651038
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69626351963232	43.49659227214539
2	12.478349174446604	13.793937399199713
3	3.9316982645331358	6.519323867524015
4	1.6531400526211364	3.654860122789754
5	0.8256130564180508	2.281642919464464
6	0.5074724284498844	1.6829252360221763
7	0.32130421787740554	1.2431272534042623
8	0.2188019240179217	0.9674805538887362
9	0.18068066502883395	0.8987839549247243
>10	1.0025603989079326	10.673497036007772
>50	0.1168001501996942	4.56265639825374
>100	0.06367746262092773	6.213995539964604
>500	0.0019492956675277702	0.7281818775487675
>1k	0.0012995304450185135	1.1305565942609588
>5k	1.2995304450185135E-4	0.6312154606331198
>10k+	2.599060890037027E-4	1.5212235139677792
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10720	0.7673937818196396	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10459	0.7487100339600382	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8788	0.6290910965140851	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3889	0.2783950016321435	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2251	0.161138377133956	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1517	0.10859481035637997	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1428	0.10222372392149676	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.4317048168276857E-4	0.0
10	0.0	0.0	0.0	1.4317048168276857E-4	0.0
11	0.0	0.0	0.0	2.1475572252415285E-4	0.0
12	0.0	0.0	0.0	5.0109668588969E-4	0.0
13	0.0	0.0	0.0	5.0109668588969E-4	0.0
14	0.0	0.0	0.0	5.726819267310743E-4	0.0
15	0.0	0.0	0.0	7.158524084138429E-4	0.0
16	0.0	0.0	0.0	0.0010737786126207643	0.0
17	0.0	0.0	0.0	0.0014317048168276858	0.0
18	0.0	0.0	0.0	0.0019328015027173758	0.0
19	0.0	0.0	0.0	0.0023623129477656816	0.0
20	0.0	0.0	0.0	0.0028634096336553716	0.0
21	0.0	0.0	0.0	0.003794017764593367	0.0
22	0.0	0.0	0.0	0.005440478303945206	0.0
23	0.0	0.0	0.0	0.008375473178441962	0.0
24	0.0	0.0	0.0	0.014460218649959626	0.0
25	0.0	0.0	0.0	0.018970088822966837	0.0
26	0.0	0.0	0.0	0.028634096336553713	0.0
27	0.0	0.0	0.0	0.08647497093639221	0.0
28	0.0	0.0	0.0	0.14574755035305842	0.0
29	0.0	0.0	0.0	0.21117646048208363	0.0
30	0.0	0.0	0.0	0.3257128458282985	0.0
31	0.0	0.0	0.0	0.5294444412628782	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3125	0.0	23.7984	1
CCCGTAT	65	6.903492E-5	19.923077	1
TAGCCCG	80	1.6170763E-5	18.5	5
CCGTATA	90	2.1528904E-6	18.5	2
CGGGCGA	70	1.2194361E-4	18.5	28
ATGACGG	95	3.6075107E-6	17.526316	20
TGCGGTC	85	2.723795E-5	17.411764	12
GTCCTAT	195	1.8189894E-12	16.128206	1
GTACGGT	70	0.0025928689	15.857142	11
CGGACCA	215	0.0	15.488372	9
TTGCGTG	120	1.9361287E-6	15.416666	7
AAGACCG	110	1.4522342E-5	15.136364	5
GTATCAA	4910	0.0	15.071283	2
GTAGACC	75	0.004104995	14.8	3
ACGGACC	215	1.2732926E-11	14.627907	8
CGCAAGA	215	1.2732926E-11	14.627907	2
CGTATAC	115	2.2104074E-5	14.478261	3
ACGCCTA	90	8.275586E-4	14.388889	6
GTATACC	90	8.275586E-4	14.388889	4
TATACCG	90	8.275586E-4	14.388889	5
>>END_MODULE