FastQCFastQC Report
Wed 25 May 2016
SRR2088420_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088420_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3212305
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT385301.1994502390028343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT377431.1749506973964179No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT341551.0632552014830472No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG175770.547177182739497No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128730.40074027839822185No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA101450.31581683557445506No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA92400.28764391924179056No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT92240.2871458345331468No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT90420.2814801209723236No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA75750.23581197924854586No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT74830.23294799217384402No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG65460.20377890642389188No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT62430.19434642725395004No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA58190.18114718247488953No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA57000.1774426774543513No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA49680.15465530203389777No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48040.1495499337702989No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT45960.14307483255792958No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA38660.12034971772605652No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36620.11399913769084816No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC35920.11182001709053158No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG33630.10469117969806728No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32660.10167154115191428No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93100.027.5810971
ATTGGAC12450.022.2891563
GTATTGG12400.022.0806451
CCCGTAT1306.9849193E-1018.51
ACGAACG1409.458745E-1118.515
GGACCCT19500.017.456416
TTGGACC20100.017.3955234
TGCGACG1502.5283953E-1017.26666622
TGGACCC21050.016.610455
TATTGGA18050.016.5013852
GTATCAA159550.016.1056082
ACGTTTA2300.015.28260926
CGTATAC2109.094947E-1214.976193
CGCAAGA4100.014.89024452
GACCCTC22000.014.8840917
ACGGACC3900.014.7051298
CGTCTGT8400.014.53571434
CGACGAG1803.3378456E-914.38888824
CGTTTAG3100.014.3225826
ATCACGT2353.6379788E-1214.17021423
Produced by FastQC (version 0.11.5)