##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088420_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3212305
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.28331525182073	31.0	31.0	34.0	30.0	34.0
2	31.511730050540034	31.0	31.0	34.0	30.0	34.0
3	31.59955577070048	31.0	31.0	34.0	30.0	34.0
4	35.40751423043578	37.0	35.0	37.0	33.0	37.0
5	35.22346321410949	37.0	35.0	37.0	33.0	37.0
6	35.26369258211783	37.0	35.0	37.0	32.0	37.0
7	35.123050582058674	37.0	35.0	37.0	32.0	37.0
8	35.156689666765764	37.0	35.0	37.0	32.0	37.0
9	36.63885683333307	39.0	35.0	39.0	32.0	39.0
10	36.526295915238435	39.0	35.0	39.0	32.0	39.0
11	36.64206667797734	39.0	35.0	39.0	32.0	39.0
12	36.50323303671351	38.0	35.0	39.0	32.0	39.0
13	36.54035062050459	39.0	35.0	39.0	32.0	39.0
14	37.51388333299609	39.0	36.0	41.0	32.0	41.0
15	37.50319723687508	39.0	36.0	41.0	32.0	41.0
16	37.46000582136503	39.0	36.0	41.0	32.0	41.0
17	37.52937688046434	39.0	36.0	41.0	32.0	41.0
18	37.51145330222379	39.0	36.0	41.0	32.0	41.0
19	37.59335088044255	39.0	36.0	41.0	32.0	41.0
20	37.55987273935694	39.0	36.0	41.0	32.0	41.0
21	37.49113175741407	39.0	36.0	41.0	32.0	41.0
22	37.396824398679456	39.0	36.0	41.0	32.0	41.0
23	37.27408854389605	39.0	36.0	41.0	31.0	41.0
24	37.20687450288811	39.0	36.0	41.0	31.0	41.0
25	37.14947615497283	39.0	36.0	41.0	31.0	41.0
26	36.91050320564205	39.0	36.0	40.0	31.0	41.0
27	36.75136545253331	39.0	35.0	40.0	30.0	41.0
28	36.636273330209924	39.0	35.0	40.0	30.0	41.0
29	36.49358544721002	38.0	35.0	40.0	30.0	41.0
30	36.31426312258643	38.0	35.0	40.0	30.0	41.0
31	36.062441766893244	38.0	35.0	40.0	30.0	41.0
32	35.9181615693404	38.0	35.0	40.0	29.0	41.0
33	35.954477236750556	38.0	35.0	40.0	30.0	41.0
34	35.89824752008293	38.0	35.0	40.0	29.0	41.0
35	35.82275001906731	38.0	35.0	40.0	29.0	41.0
36	35.737471068282744	38.0	35.0	40.0	28.0	41.0
37	35.61385298095916	38.0	34.0	40.0	27.0	41.0
38	35.480300594121665	38.0	34.0	40.0	27.0	41.0
39	35.40170127058296	38.0	34.0	40.0	26.0	41.0
40	35.22792418528129	38.0	34.0	40.0	25.0	41.0
41	35.07654347890378	38.0	34.0	40.0	24.0	41.0
42	34.91781166483258	38.0	34.0	40.0	24.0	41.0
43	34.571161206672464	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	3.0
14	4.0
15	12.0
16	32.0
17	90.0
18	220.0
19	544.0
20	1024.0
21	2205.0
22	4064.0
23	6855.0
24	11422.0
25	18260.0
26	27319.0
27	40185.0
28	55855.0
29	75137.0
30	95835.0
31	117509.0
32	143240.0
33	175222.0
34	211269.0
35	256261.0
36	323487.0
37	438017.0
38	563208.0
39	645024.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.43890446268334	22.40658966069536	13.62750423761131	22.527001639009995
2	17.82937797002464	23.138649661224573	36.2434762577028	22.788496111047984
3	19.76509079928587	26.34478980047038	32.18508205167318	21.705037348570574
4	12.850585483009864	15.999850574587407	35.959910407013034	35.189653535389695
5	11.714454262593371	40.13940145783168	34.526266964064746	13.619877315510202
6	31.239530492901512	36.66588322092703	15.864776227662066	16.22981005850939
7	24.738684527154177	35.01379850294415	21.921486284770594	18.32603068513108
8	27.574654336994776	33.38857922893374	20.554368280720542	18.482398153350942
9	25.67380743733861	13.676378799646983	23.430931994315607	37.2188817686988
10	16.33092125436408	27.557812847783758	33.773941141952584	22.33732475589958
11	31.736401120067985	22.258004766048057	24.896017034497035	21.10957707938692
12	20.239578744857663	27.48649334356482	31.830445739118794	20.44348217245872
13	30.498691749382452	21.060671387056956	28.601487094158244	19.839149769402344
14	21.00712728087775	22.6698274292136	28.435438104414118	27.887607185494527
15	23.027389989431267	29.07379591912972	26.656279525138494	21.242534566300524
16	21.950655370520543	28.483472148503957	27.112307206196174	22.453565274779326
17	19.631510706486463	27.19221867163921	28.186333489503646	24.98993713237068
18	20.765120373065447	26.328477526262294	32.04832666885616	20.858075431816093
19	20.119945023900286	25.08983424674805	33.16634005799573	21.623880671355927
20	23.959493261069543	24.20065965093601	31.899959686268893	19.939887401725553
21	23.603860779097875	25.149635542079597	30.809963561990532	20.436540116831996
22	22.028574497129007	24.79008064302736	31.797229715111115	21.384115144732522
23	21.046911796980673	26.250869702596734	32.643195462448304	20.05902303797429
24	19.944774857929122	25.674305522047252	31.414576137695516	22.96634348232811
25	21.02337729449725	26.45290531254037	32.07712219107463	20.44659520188774
26	22.243591439791675	25.799449305094008	30.844113494826924	21.112845760287392
27	21.04411007049455	26.856229405364683	30.69966892932022	21.39999159482054
28	20.76938522338321	26.496985809255346	30.823193937063888	21.91043503029756
29	19.77651561729039	26.53589867711814	31.765072121109295	21.92251358448217
30	19.51530131790101	26.867062747777688	33.13228351604222	20.48535241827909
31	20.705630380676805	26.37535974946339	32.06392294629558	20.85508692356423
32	21.5586004442293	26.321473210046992	30.503112251171665	21.61681409455204
33	22.091084128063805	26.29177490929411	30.251766255072294	21.3653747075698
34	20.911899710643915	28.56406848042138	30.180010926733296	20.34402088220141
35	19.8753231713676	29.34403800386327	30.47724297661648	20.30339584815265
36	20.51097265047995	26.520675963210216	32.03478499083991	20.933566395469917
37	20.755843545366957	26.60248637660496	31.30085717265328	21.3408129053748
38	20.81116207832071	26.093848498196774	31.86484471430951	21.230144709173008
39	21.64031746674117	25.758855401339538	31.76678428729526	20.834042844624033
40	20.210938874110646	26.31098230087118	31.164568744250627	22.31351008076755
41	20.90486426413432	25.920826322531642	31.16067745746434	22.013631955869695
42	18.997822435914397	27.418442520246366	31.50276826141976	22.080966782419477
43	19.371821791517306	27.00537464530921	31.19289731205474	22.429906251118744
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	307.0
1	411.0
2	515.0
3	1556.0
4	2597.0
5	2597.0
6	4275.0
7	5953.0
8	6535.0
9	7117.0
10	10615.5
11	14114.0
12	14114.0
13	24948.0
14	35782.0
15	57884.5
16	79987.0
17	78529.0
18	77071.0
19	77071.0
20	82994.0
21	88917.0
22	74079.0
23	59241.0
24	66029.0
25	72817.0
26	72817.0
27	78564.5
28	84312.0
29	88722.5
30	93133.0
31	99027.5
32	104922.0
33	104922.0
34	113031.5
35	121141.0
36	133592.0
37	146043.0
38	149725.5
39	153408.0
40	153408.0
41	160525.5
42	167643.0
43	185740.5
44	203838.0
45	248048.0
46	292258.0
47	292258.0
48	296518.0
49	300778.0
50	275518.5
51	250259.0
52	237072.5
53	223886.0
54	223886.0
55	196985.5
56	170085.0
57	145332.0
58	120579.0
59	107626.0
60	94673.0
61	94673.0
62	83807.0
63	72941.0
64	64256.0
65	55571.0
66	47521.0
67	39471.0
68	39471.0
69	33710.5
70	27950.0
71	23502.0
72	19054.0
73	14939.5
74	10825.0
75	10825.0
76	8359.0
77	5893.0
78	4749.0
79	3605.0
80	2948.0
81	2291.0
82	2291.0
83	1763.5
84	1236.0
85	1053.5
86	871.0
87	769.0
88	667.0
89	667.0
90	541.5
91	416.0
92	252.0
93	88.0
94	63.0
95	38.0
96	38.0
97	24.0
98	10.0
99	6.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3212305.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.45115611948499
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.77763749810896	30.581683700608846
2	13.911310719072723	11.532798248858969
3	5.166665364632986	6.424927579396132
4	2.3716582241788324	3.932319012499893
5	1.267620601145181	2.6272169719172154
6	0.7626132366445615	1.8966720198563682
7	0.5024947946001345	1.4580293126139101
8	0.3278469473416	1.087170799804259
9	0.2414026646186115	0.9005777584889167
>10	1.379087682716262	10.894494561509088
>50	0.15508237071632344	4.505866851275528
>100	0.1190260816794139	9.671929628707165
>500	0.010396679858965144	2.8675643316025616
>1k	0.006027060787768623	4.814197847620008
>5k	6.7804433862397E-4	2.088317582161684
>10k+	4.520295590826467E-4	4.716233793079375
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38530	1.1994502390028343	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37743	1.1749506973964179	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34155	1.0632552014830472	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	17577	0.547177182739497	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12873	0.40074027839822185	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10145	0.31581683557445506	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9240	0.28764391924179056	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9224	0.2871458345331468	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	9042	0.2814801209723236	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7575	0.23581197924854586	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	7483	0.23294799217384402	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6546	0.20377890642389188	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6243	0.19434642725395004	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5819	0.18114718247488953	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5700	0.1774426774543513	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4968	0.15465530203389777	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4804	0.1495499337702989	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4596	0.14307483255792958	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3866	0.12034971772605652	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3662	0.11399913769084816	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3592	0.11182001709053158	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3363	0.10469117969806728	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3266	0.10167154115191428	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.113029429023707E-5	0.0
4	0.0	0.0	0.0	3.113029429023707E-5	0.0
5	3.113029429023707E-5	0.0	0.0	3.113029429023707E-5	0.0
6	3.113029429023707E-5	0.0	0.0	3.113029429023707E-5	0.0
7	3.113029429023707E-5	0.0	0.0	3.113029429023707E-5	0.0
8	3.113029429023707E-5	0.0	0.0	3.113029429023707E-5	0.0
9	3.113029429023707E-5	0.0	0.0	1.245211771609483E-4	0.0
10	3.113029429023707E-5	0.0	0.0	1.5565147145118536E-4	0.0
11	3.113029429023707E-5	0.0	0.0	2.8017264861213365E-4	0.0
12	3.113029429023707E-5	0.0	0.0	4.35824120063319E-4	0.0
13	3.113029429023707E-5	0.0	0.0	4.669544143535561E-4	0.0
14	3.113029429023707E-5	0.0	0.0	6.537361800949786E-4	0.0
15	6.226058858047414E-5	0.0	0.0	9.339088287071122E-4	0.0
16	1.245211771609483E-4	0.0	0.0	0.0014942541259313795	0.0
17	1.245211771609483E-4	0.0	0.0	0.0024592932489287287	0.0
18	1.245211771609483E-4	0.0	0.0	0.0027705961918310994	0.0
19	1.245211771609483E-4	0.0	0.0	0.003517723254796789	0.0
20	1.245211771609483E-4	0.0	0.0	0.004545022966374612	0.0
21	1.245211771609483E-4	0.0	0.0	0.006039277092305992	0.0
22	1.245211771609483E-4	0.0	0.0	0.010522039470100131	0.0
23	1.245211771609483E-4	0.0	0.0	0.017059401271049917	0.0
24	1.245211771609483E-4	0.0	0.0	0.027581440741150046	0.0
25	1.245211771609483E-4	0.0	0.0	0.032998111947651294	0.0
26	1.245211771609483E-4	0.0	0.0	0.04183911552607863	0.0
27	1.245211771609483E-4	0.0	0.0	0.1036950102807797	0.0
28	1.245211771609483E-4	0.0	0.0	0.1711232277134332	0.0
29	1.245211771609483E-4	0.0	0.0	0.2507856508021499	0.0
30	1.245211771609483E-4	0.0	0.0	0.3883815515649977	0.0
31	1.245211771609483E-4	0.0	0.0	0.5897634253285413	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9310	0.0	27.581097	1
ATTGGAC	1245	0.0	22.289156	3
GTATTGG	1240	0.0	22.080645	1
CCCGTAT	130	6.9849193E-10	18.5	1
ACGAACG	140	9.458745E-11	18.5	15
GGACCCT	1950	0.0	17.45641	6
TTGGACC	2010	0.0	17.395523	4
TGCGACG	150	2.5283953E-10	17.266666	22
TGGACCC	2105	0.0	16.61045	5
TATTGGA	1805	0.0	16.501385	2
GTATCAA	15955	0.0	16.105608	2
ACGTTTA	230	0.0	15.282609	26
CGTATAC	210	9.094947E-12	14.97619	3
CGCAAGA	410	0.0	14.8902445	2
GACCCTC	2200	0.0	14.884091	7
ACGGACC	390	0.0	14.705129	8
CGTCTGT	840	0.0	14.535714	34
CGACGAG	180	3.3378456E-9	14.388888	24
CGTTTAG	310	0.0	14.32258	26
ATCACGT	235	3.6379788E-12	14.170214	23
>>END_MODULE