FastQCFastQC Report
Wed 25 May 2016
SRR2088419_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088419_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2368273
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT300451.268645971135929No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT285071.20370413377174No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT258041.0895703324743389No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG129700.5476564568358463No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97150.41021453185506906No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT66990.2828643488314059No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT66580.2811331294998507No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA64910.2740815775883946No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA64390.2718858847776418No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA55870.2359103025706918No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT54390.22966102303239533No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG48940.20664847338123601No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT43160.1822425032924836No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT37620.15884992988561705No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA37290.15745650944802395No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA36640.15471189343458294No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA34910.14740699235265528No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT33650.14208665977275423No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA29030.12257877364645038No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC25680.10843344496179283No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24290.10256418917920357No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC24240.10235306487047734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73150.027.0102541
ATTGGAC6100.021.8360653
GTATTGG6750.020.829631
CGCGAAT450.003826988220.55555735
TTGGACC11050.018.751134
GGACCCT12100.017.4297526
TGGACCC12450.017.2369465
GACCCTC13150.016.4600757
TATACGT803.3844615E-416.18754
CGTCTGT6150.015.943089534
GTATCAA124700.015.8889322
TATACTG3450.015.5507255
TATACCG1201.937513E-615.4166665
ACCCTCG13700.015.1240888
GTATAGA1853.0559022E-1015.01
CAATGCG2109.094947E-1214.9761919
CGACGAG2350.014.95744624
GTACTAG3500.014.81
TGCGACG2006.184564E-1114.79999922
ACGTTTA1651.6474587E-814.57575726
Produced by FastQC (version 0.11.5)