##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088419_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2368273
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.200596806195907	31.0	31.0	34.0	30.0	34.0
2	31.42481546679796	31.0	31.0	34.0	30.0	34.0
3	31.510094908821745	31.0	31.0	34.0	30.0	34.0
4	35.32546670084066	37.0	35.0	37.0	33.0	37.0
5	35.14014727187279	37.0	35.0	37.0	32.0	37.0
6	35.189470555126036	37.0	35.0	37.0	32.0	37.0
7	35.05379531836068	37.0	35.0	37.0	32.0	37.0
8	35.093900492046316	37.0	35.0	37.0	32.0	37.0
9	36.563510203426716	39.0	35.0	39.0	32.0	39.0
10	36.4393475752162	38.0	35.0	39.0	32.0	39.0
11	36.55882535501608	39.0	35.0	39.0	32.0	39.0
12	36.41434243433928	38.0	35.0	39.0	32.0	39.0
13	36.46063101677889	38.0	35.0	39.0	32.0	39.0
14	37.42791730514176	39.0	36.0	41.0	32.0	41.0
15	37.41309046718854	39.0	36.0	41.0	32.0	41.0
16	37.359934433234685	39.0	36.0	41.0	32.0	41.0
17	37.43235978284598	39.0	36.0	41.0	32.0	41.0
18	37.41047294800895	39.0	36.0	41.0	32.0	41.0
19	37.492380734822376	39.0	36.0	41.0	32.0	41.0
20	37.45937271589889	39.0	36.0	41.0	32.0	41.0
21	37.39940918973446	39.0	36.0	41.0	32.0	41.0
22	37.303004341138035	39.0	36.0	41.0	31.0	41.0
23	37.19310569347368	39.0	36.0	41.0	31.0	41.0
24	37.13282336960308	39.0	36.0	41.0	31.0	41.0
25	37.0640808724332	39.0	36.0	41.0	31.0	41.0
26	36.813058714092506	39.0	35.0	40.0	30.0	41.0
27	36.65899370553986	39.0	35.0	40.0	30.0	41.0
28	36.538202310291084	38.0	35.0	40.0	30.0	41.0
29	36.39688583199656	38.0	35.0	40.0	30.0	41.0
30	36.20830199896718	38.0	35.0	40.0	30.0	41.0
31	35.949909913257464	38.0	35.0	40.0	29.0	41.0
32	35.81409237870803	38.0	34.0	40.0	29.0	41.0
33	35.8508888122273	38.0	35.0	40.0	29.0	41.0
34	35.78557961856593	38.0	35.0	40.0	29.0	41.0
35	35.69589021198147	38.0	34.0	40.0	28.0	41.0
36	35.60834667287091	38.0	34.0	40.0	27.0	41.0
37	35.48017141604874	38.0	34.0	40.0	27.0	41.0
38	35.35464703604694	38.0	34.0	40.0	26.0	41.0
39	35.27548639873866	38.0	34.0	40.0	25.0	41.0
40	35.097207543218204	38.0	34.0	40.0	25.0	41.0
41	34.94599144608751	38.0	34.0	40.0	24.0	41.0
42	34.79257036667648	38.0	33.0	40.0	24.0	41.0
43	34.44632692261408	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	3.0
15	11.0
16	29.0
17	75.0
18	181.0
19	414.0
20	866.0
21	1703.0
22	3191.0
23	5554.0
24	8983.0
25	14337.0
26	21532.0
27	31172.0
28	43084.0
29	58445.0
30	73021.0
31	89167.0
32	108636.0
33	130906.0
34	159471.0
35	192072.0
36	240277.0
37	321334.0
38	414532.0
39	449273.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.331848144196215	22.450114492712622	13.779534707358485	22.438502655732677
2	18.10441617161535	23.26682776858918	36.003239491393096	22.625516568402375
3	20.186101855656	26.346582509702216	31.69026543814839	21.777050196493395
4	13.287868417196835	16.288704891708008	35.323968140497314	35.09945855059784
5	11.908677758011851	40.19114350414838	34.15370609722781	13.746472640611957
6	31.46710704382476	36.20756559737834	15.875450169807282	16.449877188989614
7	24.882182079515324	34.71174987005299	21.960939469393942	18.445128581037743
8	27.450931543787394	33.70282902351207	20.48154921328749	18.36469021941305
9	25.758770209346643	13.739125514668283	23.16616369818851	37.33594057779656
10	16.496240087185896	27.631738401780538	33.57454989353001	22.297471617503557
11	31.959237807465605	22.19794761837001	24.585045727414027	21.257768846750352
12	20.364628571114903	27.42960798860604	31.77995104449529	20.425812395783762
13	30.77491488523494	21.10217023121912	28.28905282456879	19.833862058977154
14	21.049980302101996	22.411563193939212	28.51297126640383	28.02548523755496
15	23.222533888618415	28.83953834714157	26.653008331387472	21.284919432852547
16	21.822610822316516	28.256835255057165	27.233811304693333	22.686742617932985
17	19.81355190047769	27.112752626069714	28.183195096173456	24.89050037727914
18	20.769733894698795	26.237008993473303	31.898307331967217	21.094949779860684
19	20.279672149283464	25.041623157465377	33.16682662851791	21.511878064733246
20	23.846026197148724	24.190327719819464	32.10187339044105	19.861772692590762
21	23.41799277363716	24.990995548232824	30.95770631173011	20.633305366399902
22	22.127220974946724	24.608058277065187	31.86098055418442	21.40374019380367
23	21.034061529224036	26.19512193062202	32.73845540611239	20.032361134041558
24	20.128211570203266	25.52488670013972	31.4272045494755	22.919697180181508
25	21.116906707968212	26.296419373948865	32.06678453033075	20.519889387752173
26	22.25685130050463	25.532191601221648	31.02813738112118	21.18281971715254
27	21.12704067478707	26.71410770633284	30.71563117934461	21.443220439535477
28	20.85338134581613	26.261583863009037	30.985954744237677	21.899080046937154
29	20.061622963231013	26.164593355580205	31.88593544747586	21.88784823371292
30	19.708749793625987	26.4742282667581	33.11923920933102	20.697782730284896
31	20.708718969476912	25.996876204728085	32.28301804732816	21.01138677846684
32	21.53307494532936	26.16294658597214	30.716602351164752	21.587376117533747
33	22.312503668284865	25.965883156207077	30.379225705820232	21.342387469687825
34	20.99994384093388	28.122095721228085	30.38467271298537	20.493287724852667
35	20.037808141206696	28.842451862601987	30.581905042197416	20.5378349539939
36	20.7034830866205	26.04315465320088	32.0996776976303	21.15368456254832
37	20.724637742354872	26.264159579575498	31.397182672774633	21.614020005294996
38	20.922883468248806	25.81518262463829	31.97004737207239	21.291886535040515
39	21.690278105606914	25.34192637419757	31.88445757731478	21.08333794288074
40	20.352510035794015	26.027447004631643	31.229465521922517	22.390577437651825
41	20.908991488734618	25.733055268543787	31.15054725532065	22.207405987400946
42	19.027747223398652	27.022180297626157	31.554723631946146	22.395348847029037
43	19.648199341883306	26.807551325375073	31.020156882251328	22.524092450490294
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	424.0
1	476.5
2	529.0
3	1337.5
4	2146.0
5	2146.0
6	3536.0
7	4926.0
8	5278.0
9	5630.0
10	8251.5
11	10873.0
12	10873.0
13	19133.5
14	27394.0
15	44037.0
16	60680.0
17	59375.0
18	58070.0
19	58070.0
20	62037.5
21	66005.0
22	53847.0
23	41689.0
24	45120.0
25	48551.0
26	48551.0
27	53538.5
28	58526.0
29	61940.5
30	65355.0
31	70010.0
32	74665.0
33	74665.0
34	80638.0
35	86611.0
36	93992.0
37	101373.0
38	106731.0
39	112089.0
40	112089.0
41	117573.0
42	123057.0
43	137608.0
44	152159.0
45	184594.5
46	217030.0
47	217030.0
48	220802.0
49	224574.0
50	205556.5
51	186539.0
52	176190.0
53	165841.0
54	165841.0
55	145957.0
56	126073.0
57	108750.5
58	91428.0
59	81459.0
60	71490.0
61	71490.0
62	63120.5
63	54751.0
64	48075.5
65	41400.0
66	36060.0
67	30720.0
68	30720.0
69	26128.5
70	21537.0
71	18634.0
72	15731.0
73	12400.0
74	9069.0
75	9069.0
76	7080.0
77	5091.0
78	3888.0
79	2685.0
80	2183.0
81	1681.0
82	1681.0
83	1255.0
84	829.0
85	643.0
86	457.0
87	403.5
88	350.0
89	350.0
90	269.0
91	188.0
92	112.5
93	37.0
94	26.0
95	15.0
96	15.0
97	10.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2368273.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.17361137682731
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.61575952587246	34.1583693478539
2	13.197555671954698	11.923625020978491
3	4.591625181520032	6.222608746141202
4	2.0811248396282105	3.7604769892810737
5	1.0990157191988017	2.4823254498055523
6	0.7024155344489209	1.9038387826945207
7	0.47155326871609704	1.4911234873117512
8	0.31665779887233314	1.1443661076560259
9	0.23921391877906856	0.9725540942568219
>10	1.446894252224211	12.48381582765255
>50	0.143122450371216	4.524762403946929
>100	0.0840140975641678	6.925993013428347
>500	0.004681246122853027	1.48036613930854
>1k	0.005336620580052439	4.420501357476402
>5k	6.553744571994223E-4	1.9889908805040257
>10k+	3.7449968982824133E-4	4.116282351703981
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	30045	1.268645971135929	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28507	1.20370413377174	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25804	1.0895703324743389	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12970	0.5476564568358463	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9715	0.41021453185506906	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6699	0.2828643488314059	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6658	0.2811331294998507	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6491	0.2740815775883946	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6439	0.2718858847776418	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5587	0.2359103025706918	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5439	0.22966102303239533	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4894	0.20664847338123601	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4316	0.1822425032924836	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3762	0.15884992988561705	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3729	0.15745650944802395	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3664	0.15471189343458294	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3491	0.14740699235265528	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3365	0.14208665977275423	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2903	0.12257877364645038	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	2568	0.10843344496179283	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2429	0.10256418917920357	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2424	0.10235306487047734	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	4.222486174524643E-5	0.0	0.0	0.0	0.0
7	4.222486174524643E-5	0.0	0.0	0.0	0.0
8	4.222486174524643E-5	0.0	0.0	0.0	0.0
9	4.222486174524643E-5	0.0	0.0	4.222486174524643E-5	0.0
10	4.222486174524643E-5	0.0	0.0	8.444972349049286E-5	0.0
11	4.222486174524643E-5	0.0	0.0	3.800237557072179E-4	0.0
12	4.222486174524643E-5	0.0	0.0	7.178226496691893E-4	0.0
13	4.222486174524643E-5	0.0	0.0	8.444972349049286E-4	0.0
14	4.222486174524643E-5	0.0	0.0	9.711718201406679E-4	0.0
15	4.222486174524643E-5	0.0	0.0	0.0013511955758478858	0.0
16	8.444972349049286E-5	0.0	0.0	0.0024912668429695395	0.0
17	8.444972349049286E-5	0.0	0.0	0.0036735629718364395	0.0
18	8.444972349049286E-5	0.0	0.0	0.004053586727543657	0.0
19	8.444972349049286E-5	0.0	0.0	0.004771409377212847	0.0
20	8.444972349049286E-5	0.0	0.0	0.006375954123532211	0.0
21	8.444972349049286E-5	0.0	0.0	0.009500593892680447	0.0
22	8.444972349049286E-5	0.0	0.0	0.016003222601448396	0.0
23	8.444972349049286E-5	0.0	0.0	0.026221639143798034	0.0
24	8.444972349049286E-5	0.0	0.0	0.03926912142307918	0.0
25	8.444972349049286E-5	0.0	0.0	0.045729525270101885	0.0
26	8.444972349049286E-5	0.0	0.0	0.056243515844668246	0.0
27	8.444972349049286E-5	0.0	0.0	0.12705460899144652	0.0
28	8.444972349049286E-5	0.0	0.0	0.20812634354231965	0.0
29	8.444972349049286E-5	0.0	0.0	0.29988096811474013	0.0
30	8.444972349049286E-5	0.0	0.0	0.46628914825275636	0.0
31	8.444972349049286E-5	0.0	0.0	0.7106444231724974	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7315	0.0	27.010254	1
ATTGGAC	610	0.0	21.836065	3
GTATTGG	675	0.0	20.82963	1
CGCGAAT	45	0.0038269882	20.555557	35
TTGGACC	1105	0.0	18.75113	4
GGACCCT	1210	0.0	17.429752	6
TGGACCC	1245	0.0	17.236946	5
GACCCTC	1315	0.0	16.460075	7
TATACGT	80	3.3844615E-4	16.1875	4
CGTCTGT	615	0.0	15.9430895	34
GTATCAA	12470	0.0	15.888932	2
TATACTG	345	0.0	15.550725	5
TATACCG	120	1.937513E-6	15.416666	5
ACCCTCG	1370	0.0	15.124088	8
GTATAGA	185	3.0559022E-10	15.0	1
CAATGCG	210	9.094947E-12	14.97619	19
CGACGAG	235	0.0	14.957446	24
GTACTAG	350	0.0	14.8	1
TGCGACG	200	6.184564E-11	14.799999	22
ACGTTTA	165	1.6474587E-8	14.575757	26
>>END_MODULE