FastQCFastQC Report
Wed 25 May 2016
SRR2088418_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088418_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3378256
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT365881.083044032187022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT358521.0612576429968599No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT323490.9575650868377056No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG162410.48075101472475734No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122320.3620803159973667No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA87810.25992701559621295No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT85420.25285235932386413No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT81690.24181115936743694No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA78880.23349325805977994No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA69220.2048986222476923No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT66100.19566308769968882No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT60880.18021132797514458No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG60690.17964890760202898No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA50100.14830137206890182No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA48830.1445420358907081No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48350.14312118442178448No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT43800.12965269653927944No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA43560.12894227080481765No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA38410.11369771858615807No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC34250.10138367252215344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82300.028.2332921
ATTGGAC8150.018.1595123
GTATTGG8750.017.9714281
TTGGACC14700.016.6122464
GTATCAA142200.016.3403642
GGACCCT14250.016.0982466
TCGACGG1302.593115E-715.65384619
ACGACGC1455.3552867E-815.31034527
ACGTGCG1353.9784754E-715.0740759
TGGACCC14800.015.05
CCCGTAT1508.115603E-814.81
CGCGATA1252.9626754E-614.79999914
AAGACCG2301.8189894E-1214.4782615
CGCATAC908.2798715E-414.38888837
CGAATTA2850.014.28070215
CGTCTGT8100.014.16049434
ATAAGAC2900.014.0344833
CCGATAA1854.9094524E-914.09
GACCCTC16000.013.8750017
AATGCGT3150.013.507936535
Produced by FastQC (version 0.11.5)