##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088418_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3378256
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.259025958956336	31.0	31.0	34.0	30.0	34.0
2	31.48526458622437	31.0	31.0	34.0	30.0	34.0
3	31.57691424214151	31.0	31.0	34.0	30.0	34.0
4	35.386777970645205	37.0	35.0	37.0	33.0	37.0
5	35.19527472163151	37.0	35.0	37.0	32.0	37.0
6	35.234835370676464	37.0	35.0	37.0	32.0	37.0
7	35.10845004049427	37.0	35.0	37.0	32.0	37.0
8	35.1347650977309	37.0	35.0	37.0	32.0	37.0
9	36.62520010324854	39.0	35.0	39.0	32.0	39.0
10	36.50169288532308	38.0	35.0	39.0	32.0	39.0
11	36.623408646354804	39.0	35.0	39.0	32.0	39.0
12	36.47957733220928	38.0	35.0	39.0	32.0	39.0
13	36.52169048171601	38.0	35.0	39.0	32.0	39.0
14	37.50523406159865	39.0	36.0	41.0	32.0	41.0
15	37.493295653141736	39.0	36.0	41.0	32.0	41.0
16	37.45455228970214	39.0	36.0	41.0	32.0	41.0
17	37.5036237632672	39.0	36.0	41.0	32.0	41.0
18	37.4839878919774	39.0	36.0	41.0	32.0	41.0
19	37.56392913976916	39.0	36.0	41.0	32.0	41.0
20	37.526131530588565	39.0	36.0	41.0	32.0	41.0
21	37.46395625435136	39.0	36.0	41.0	32.0	41.0
22	37.365375211351655	39.0	36.0	41.0	31.0	41.0
23	37.25116243410801	39.0	36.0	41.0	31.0	41.0
24	37.18356009728097	39.0	36.0	41.0	31.0	41.0
25	37.12480581696591	39.0	36.0	41.0	31.0	41.0
26	36.886956761121716	39.0	36.0	40.0	31.0	41.0
27	36.733898496739144	39.0	35.0	40.0	30.0	41.0
28	36.62339384581867	39.0	35.0	40.0	30.0	41.0
29	36.4775842920134	38.0	35.0	40.0	30.0	41.0
30	36.301066585836004	38.0	35.0	40.0	30.0	41.0
31	36.0647775065004	38.0	35.0	40.0	30.0	41.0
32	35.922540802118014	38.0	35.0	40.0	29.0	41.0
33	35.977382116689796	38.0	35.0	40.0	30.0	41.0
34	35.93176656831217	38.0	35.0	40.0	29.0	41.0
35	35.868168664541706	38.0	35.0	40.0	29.0	41.0
36	35.789013621229415	38.0	35.0	40.0	28.0	41.0
37	35.666789609786825	38.0	34.0	40.0	27.0	41.0
38	35.54461503213492	38.0	34.0	40.0	27.0	41.0
39	35.4761951136918	38.0	34.0	40.0	26.0	41.0
40	35.32253209940277	38.0	34.0	40.0	26.0	41.0
41	35.18748312738881	38.0	34.0	40.0	25.0	41.0
42	35.047398716971124	38.0	34.0	40.0	24.0	41.0
43	34.70301392197631	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	3.0
14	3.0
15	17.0
16	37.0
17	92.0
18	234.0
19	515.0
20	1107.0
21	2237.0
22	4103.0
23	7313.0
24	11962.0
25	18898.0
26	28586.0
27	41865.0
28	58419.0
29	78482.0
30	100456.0
31	123675.0
32	150853.0
33	182863.0
34	223269.0
35	270940.0
36	340986.0
37	457156.0
38	602109.0
39	672074.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.091734907005275	22.401262663338716	13.502173902747453	23.004828526908558
2	18.03116756101373	23.23545048095822	35.66124059277923	23.072141365248815
3	19.82484453516844	26.16867993426194	32.00189683671101	22.004578693858605
4	13.171352319066406	16.3046850209102	36.016068646070636	34.507894013952765
5	11.953653009126603	39.94934072491842	34.51638952169404	13.580616744260945
6	31.154003722630847	36.946371145348365	15.996182645720157	15.903442486300623
7	24.86256222145391	34.73138210958554	22.18017225455975	18.225883414400805
8	27.40141658891452	33.382224437698035	20.631414552360745	18.58494442102671
9	25.817285605353767	13.610602630469687	23.122137576311562	37.44997418786498
10	16.072050193946225	27.62694715853387	34.11147645412307	22.18952619339683
11	32.162186643048955	22.199176142956603	24.775298260404185	20.863338953590254
12	20.321846538569012	27.280437006550123	31.810821915213054	20.586894539667806
13	30.34133588455108	21.145999592689247	28.247208026863564	20.26545649589611
14	20.97327733599822	22.492256359494366	28.49878754007985	28.035678764427562
15	23.13010026475199	29.163598022174753	26.304341648471873	21.40196006460138
16	21.991939035999643	28.378399979160847	27.020806001676608	22.60885498316291
17	19.965242420941458	27.227687895766334	28.065723852780845	24.741345830511364
18	20.84362463945894	26.50000473617156	31.586682595990357	21.06968802837914
19	20.35710141564168	25.458609412667364	32.642020024533366	21.542269147157587
20	23.726887482772177	24.636528433605978	31.474879346029432	20.161704737592416
21	23.376558792465698	25.404261843981036	30.54055110092308	20.678628262630188
22	22.1876317247716	25.077377202911798	31.34087529186657	21.394115780450033
23	21.267452792209944	26.430767828133806	31.958176052969346	20.343603326686907
24	20.274928839022266	25.990333473839755	30.871224679242783	22.863513007895197
25	21.235986852387743	26.571136112834555	31.55681511407069	20.636061920707014
26	22.229073225948536	25.948980775879626	30.54436963924581	21.277576358926027
27	21.244807971923976	27.045611700238233	30.263248255904823	21.446332071932975
28	20.898268218867962	26.64889812968585	30.43999625842447	22.012837393021726
29	20.082906683211693	26.620865914246878	31.38228719197124	21.91394021057019
30	19.829403100297903	26.911933257870334	32.49540591358382	20.763257728247947
31	20.811477874974543	26.497133432161448	31.649999289574264	21.041389403289745
32	21.708597572238457	26.427304502678307	30.183976584367795	21.68012134071545
33	22.20160343088268	26.511608356501103	29.829474142871355	21.45731406974486
34	21.163671432833983	28.329735816350208	29.879884768945868	20.626707981869934
35	20.102354587692584	28.93925149544617	30.242231494593657	20.716162422267583
36	20.7642641647051	26.498998299714412	31.516616857929066	21.220120677651426
37	20.911795908895	26.43227748281954	30.934778181404845	21.721148426880614
38	20.871124035597067	26.16788070531067	31.47727703288324	21.483718226209024
39	21.700753288087107	25.777916179235678	31.426570396086028	21.094760136591187
40	20.331970105285095	26.195113691798372	31.033468156350498	22.43944804656604
41	20.91194391425635	25.80434993677211	31.025683074343686	22.258023074627857
42	19.22486040134318	27.00633107733695	31.345404255923764	22.423404265396112
43	19.56266783807977	26.63827134474119	31.093617535201595	22.70544328197745
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	383.0
1	500.5
2	618.0
3	1637.0
4	2656.0
5	2656.0
6	4466.5
7	6277.0
8	6710.0
9	7143.0
10	10673.0
11	14203.0
12	14203.0
13	24587.0
14	34971.0
15	56440.5
16	77910.0
17	76474.0
18	75038.0
19	75038.0
20	81101.0
21	87164.0
22	73454.5
23	59745.0
24	66861.0
25	73977.0
26	73977.0
27	80339.5
28	86702.0
29	91615.0
30	96528.0
31	104110.5
32	111693.0
33	111693.0
34	122415.5
35	133138.0
36	145121.0
37	157104.0
38	162917.0
39	168730.0
40	168730.0
41	177895.5
42	187061.0
43	204057.0
44	221053.0
45	261115.5
46	301178.0
47	301178.0
48	305503.5
49	309829.0
50	283566.0
51	257303.0
52	243383.0
53	229463.0
54	229463.0
55	203560.5
56	177658.0
57	154986.5
58	132315.0
59	118328.0
60	104341.0
61	104341.0
62	93061.0
63	81781.0
64	71982.0
65	62183.0
66	52826.5
67	43470.0
68	43470.0
69	36460.0
70	29450.0
71	24903.0
72	20356.0
73	15960.0
74	11564.0
75	11564.0
76	8972.0
77	6380.0
78	5062.5
79	3745.0
80	3030.5
81	2316.0
82	2316.0
83	1749.5
84	1183.0
85	949.0
86	715.0
87	618.5
88	522.0
89	522.0
90	410.0
91	298.0
92	188.0
93	78.0
94	52.0
95	26.0
96	26.0
97	16.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3378256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.89300421409602
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.4214329452219	30.339513955650528
2	14.151807147011496	11.857234328936533
3	5.545634364337957	6.969698513851371
4	2.568015486227967	4.303275343456482
5	1.4347891422159527	3.005381379059606
6	0.8757289247433613	2.201214932080765
7	0.5810191113292221	1.7038445255569796
8	0.3874980499477513	1.2986765951532107
9	0.2787439162486186	1.0509678052251303
>10	1.4811070941483298	11.60354605360176
>50	0.14802075879136783	4.31573000892775
>100	0.11205520319880241	9.056144809761017
>500	0.007993538966376081	2.2604840952239176
>1k	0.005163967650496283	4.186124463442042
>5k	6.366535459515966E-4	1.8989690386522624
>10k+	3.5369641441755363E-4	3.9491941514205555
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36588	1.083044032187022	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35852	1.0612576429968599	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32349	0.9575650868377056	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16241	0.48075101472475734	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12232	0.3620803159973667	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8781	0.25992701559621295	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8542	0.25285235932386413	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8169	0.24181115936743694	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7888	0.23349325805977994	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6922	0.2048986222476923	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6610	0.19566308769968882	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6088	0.18021132797514458	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6069	0.17964890760202898	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5010	0.14830137206890182	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4883	0.1445420358907081	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4835	0.14312118442178448	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4380	0.12965269653927944	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4356	0.12894227080481765	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3841	0.11369771858615807	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3425	0.10138367252215344	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.960107226924188E-5	0.0
5	0.0	0.0	0.0	2.960107226924188E-5	0.0
6	2.960107226924188E-5	0.0	0.0	2.960107226924188E-5	0.0
7	2.960107226924188E-5	0.0	0.0	2.960107226924188E-5	0.0
8	2.960107226924188E-5	0.0	0.0	8.880321680772565E-5	0.0
9	2.960107226924188E-5	0.0	0.0	8.880321680772565E-5	0.0
10	2.960107226924188E-5	0.0	0.0	1.1840428907696752E-4	0.0
11	2.960107226924188E-5	0.0	0.0	3.256117949616607E-4	0.0
12	2.960107226924188E-5	0.0	0.0	5.328193008463538E-4	0.0
13	2.960107226924188E-5	0.0	0.0	5.624203731155957E-4	0.0
14	2.960107226924188E-5	0.0	0.0	6.216225176540795E-4	0.0
15	2.960107226924188E-5	0.0	0.0	7.400268067310471E-4	0.0
16	2.960107226924188E-5	0.0	0.0	0.0013024471798466428	0.0
17	2.960107226924188E-5	0.0	0.0	0.0020424739865776897	0.0
18	2.960107226924188E-5	0.0	0.0	0.002486490070616318	0.0
19	2.960107226924188E-5	0.0	0.0	0.003019309371462672	0.0
20	2.960107226924188E-5	0.0	0.0	0.0035521286723090256	0.0
21	2.960107226924188E-5	0.0	0.0	0.004795373707617185	0.0
22	2.960107226924188E-5	0.0	0.0	0.007992289512695308	0.0
23	2.960107226924188E-5	0.0	0.0	0.013320482521158847	0.0
24	2.960107226924188E-5	0.0	0.0	0.02172718704562354	0.0
25	2.960107226924188E-5	0.0	0.0	0.02675936933139466	0.0
26	2.960107226924188E-5	0.0	0.0	0.03605410602393661	0.0
27	2.960107226924188E-5	0.0	0.0	0.10218290147342297	0.0
28	2.960107226924188E-5	0.0	0.0	0.16739406368256285	0.0
29	2.960107226924188E-5	0.0	0.0	0.240597515404398	0.0
30	2.960107226924188E-5	0.0	0.0	0.3745127663504483	0.0
31	2.960107226924188E-5	0.0	0.0	0.5848283848234118	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8230	0.0	28.233292	1
ATTGGAC	815	0.0	18.159512	3
GTATTGG	875	0.0	17.971428	1
TTGGACC	1470	0.0	16.612246	4
GTATCAA	14220	0.0	16.340364	2
GGACCCT	1425	0.0	16.098246	6
TCGACGG	130	2.593115E-7	15.653846	19
ACGACGC	145	5.3552867E-8	15.310345	27
ACGTGCG	135	3.9784754E-7	15.074075	9
TGGACCC	1480	0.0	15.0	5
CCCGTAT	150	8.115603E-8	14.8	1
CGCGATA	125	2.9626754E-6	14.799999	14
AAGACCG	230	1.8189894E-12	14.478261	5
CGCATAC	90	8.2798715E-4	14.388888	37
CGAATTA	285	0.0	14.280702	15
CGTCTGT	810	0.0	14.160494	34
ATAAGAC	290	0.0	14.034483	3
CCGATAA	185	4.9094524E-9	14.0	9
GACCCTC	1600	0.0	13.875001	7
AATGCGT	315	0.0	13.5079365	35
>>END_MODULE