FastQCFastQC Report
Wed 25 May 2016
SRR2088417_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088417_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences623388
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61360.984298703215333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60250.9664927781734649No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54070.8673570873998216No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG23220.3724807022271843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21350.3424833330125059No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT13300.213350273024184No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA13020.208858688328938No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA12620.2024421387643009No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT11700.18768407476563553No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA10600.17003856346288349No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT10550.16923649476730385No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT9800.15720546433360927No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG9070.1454952613781465No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA8090.1297747149447856No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7840.1257643714668874No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT7440.11934782190225028No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA7290.11694161581551138No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA7190.11533747842435207No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATACGG303.597605E-430.8333345
GGTATCA16350.027.0428121
GCTAACG400.001930201423.12526
TGTACTG951.6730337E-719.4736845
GTTAGAC500.007031359218.53
ACCCGTT609.2303514E-418.530
GGACCAA500.007031359218.56
TATACTG609.2303514E-418.55
GTATTGG1850.018.01
TAATACT1258.556526E-917.764
TAGTGTG650.001578837717.0769235
CGCCGGA650.001578837717.07692314
TTTACAC1107.7935147E-716.8181823
GCTTTAT1107.7935147E-716.8181821
AATACCG1352.2135282E-816.4444455
TTGGACC2750.016.1454544
GCCGGAA700.00259070915.85714315
GGACCCT2450.015.8571426
TAAGCAG1302.5832014E-715.6538465
GTATCAA28400.015.5686632
Produced by FastQC (version 0.11.5)