##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088417_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	623388
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48172085442774	31.0	31.0	34.0	30.0	34.0
2	31.693771776165086	31.0	31.0	34.0	30.0	34.0
3	31.785953531348053	31.0	31.0	34.0	30.0	34.0
4	35.55339210892735	37.0	35.0	37.0	33.0	37.0
5	35.385795042573804	37.0	35.0	37.0	33.0	37.0
6	35.417011556205765	37.0	35.0	37.0	33.0	37.0
7	35.298932606979925	37.0	35.0	37.0	32.0	37.0
8	35.321700128972644	37.0	35.0	37.0	32.0	37.0
9	36.86353442799669	39.0	37.0	39.0	33.0	39.0
10	36.7330715381111	39.0	35.0	39.0	32.0	39.0
11	36.833537058782014	39.0	37.0	39.0	32.0	39.0
12	36.6984510449351	39.0	35.0	39.0	32.0	39.0
13	36.72589462742305	39.0	35.0	39.0	32.0	39.0
14	37.75862705088966	40.0	37.0	41.0	32.0	41.0
15	37.75883077633833	40.0	37.0	41.0	32.0	41.0
16	37.7249048746527	40.0	37.0	41.0	32.0	41.0
17	37.77474061098385	40.0	37.0	41.0	32.0	41.0
18	37.75028874473041	40.0	37.0	41.0	32.0	41.0
19	37.8201665736267	40.0	37.0	41.0	32.0	41.0
20	37.781915917534505	40.0	37.0	41.0	32.0	41.0
21	37.739629251766154	40.0	37.0	41.0	32.0	41.0
22	37.62446662431744	39.0	36.0	41.0	32.0	41.0
23	37.515348386558614	39.0	36.0	41.0	32.0	41.0
24	37.44988033135062	39.0	36.0	41.0	32.0	41.0
25	37.39592998261115	39.0	36.0	41.0	32.0	41.0
26	37.190505752436685	39.0	36.0	41.0	31.0	41.0
27	37.03009522159554	39.0	36.0	41.0	31.0	41.0
28	36.932526773053056	39.0	35.0	40.0	31.0	41.0
29	36.78574178521242	39.0	35.0	40.0	30.0	41.0
30	36.602664793034194	38.0	35.0	40.0	30.0	41.0
31	36.400532252786384	38.0	35.0	40.0	30.0	41.0
32	36.24209801921115	38.0	35.0	40.0	30.0	41.0
33	36.32300750094644	38.0	35.0	40.0	30.0	41.0
34	36.28445205874993	38.0	35.0	40.0	30.0	41.0
35	36.23426180805534	38.0	35.0	40.0	30.0	41.0
36	36.159205823660386	38.0	35.0	40.0	30.0	41.0
37	36.03156781972062	38.0	35.0	40.0	29.0	41.0
38	35.934762940576334	38.0	35.0	40.0	29.0	41.0
39	35.889154106270894	38.0	35.0	40.0	28.0	41.0
40	35.75761965260801	38.0	35.0	40.0	27.0	41.0
41	35.63361983227139	38.0	35.0	40.0	27.0	41.0
42	35.49834132193754	38.0	35.0	40.0	26.0	41.0
43	35.164189878534714	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	2.0
15	2.0
16	6.0
17	12.0
18	30.0
19	61.0
20	193.0
21	297.0
22	580.0
23	976.0
24	1838.0
25	2777.0
26	4382.0
27	6374.0
28	9179.0
29	12522.0
30	16455.0
31	20367.0
32	25413.0
33	31567.0
34	38856.0
35	48032.0
36	61852.0
37	84569.0
38	115733.0
39	141313.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.21478116357709	22.17158495190796	13.48052898034611	23.13310490416883
2	17.762132091089338	22.977824404704613	36.21564739776832	23.044396106437723
3	19.389208646942194	26.23662951484469	31.77234723799624	22.60181460021688
4	12.904483243180811	16.36204097608552	36.63865842781703	34.09481735291664
5	12.025095125347296	39.743947589623154	34.81138552554749	13.419571759482057
6	31.077114092667806	37.2244894030684	16.173875660102535	15.52452084416126
7	25.048605362952124	34.265818398814226	22.606627012390355	18.078949225843292
8	26.80144629027187	33.94563257553883	20.81608885637837	18.43683227781093
9	25.690581146894072	14.04133541229539	22.955687308706615	37.31239613210392
10	16.172752763928724	27.46908827247236	34.3542063690671	22.003952594531818
11	32.21332460682593	22.75260351498585	24.391550687533286	20.642521190654936
12	20.164809075567707	27.49042329977478	31.865868447900823	20.47889917675669
13	30.066026295020116	21.418763274236913	27.851514626524732	20.66369580421824
14	20.859721393417903	22.65266575551663	28.44376215134074	28.043850699724732
15	22.965151719314456	29.38523038621212	26.061297297990976	21.588320596482447
16	21.940589167581027	28.46365345499111	27.00581339390556	22.5899439835223
17	20.04016760027463	27.63094573524033	28.060694142331904	24.268192522153136
18	20.80662444577053	26.911490115305398	31.061874787451792	21.220010651472275
19	20.451147599889634	26.096748734335602	32.120765879356036	21.33133778641873
20	23.457461484661238	25.38387007770442	31.014231906934363	20.14443653069998
21	23.18138943964273	25.876661084268544	30.14109992492637	20.800849551162358
22	22.1085423524354	25.588076767598995	30.92857097024646	21.374809909719147
23	21.319146342245922	26.74899099758096	31.455530103242285	20.476332556930835
24	20.35137025415953	26.555532028207153	30.51999717671819	22.573100540915128
25	21.283053250944835	27.08088702381182	31.11721752744679	20.51884219779656
26	22.053680853657752	26.448856891695062	30.204302938138046	21.29315931650914
27	21.237816576514145	27.260710825360768	30.069876224758897	21.431596373366187
28	21.00200838001373	26.970201543821826	30.27584746578375	21.751942610380695
29	20.25752821677671	26.93025852278196	30.97460971337273	21.8376035470686
30	19.977927069497646	27.36706513439463	31.892497128594073	20.762510667513652
31	20.97329432071198	26.92111493965235	31.11577380379475	20.98981693584092
32	21.498168075099297	26.966351614083045	29.99688797346115	21.538592337356512
33	21.947807785841242	26.860638960005645	29.793964593479505	21.39758866067361
34	21.01307692801273	28.25415311170571	29.93945985485765	20.79331010542391
35	20.232022432257278	28.798597342265168	30.154253851533873	20.815126373943677
36	20.663054149261775	26.799040084185133	31.195499432135364	21.34240633441773
37	20.76363356368746	26.721560248192137	30.81243142312653	21.702374764993873
38	20.891643727501975	26.52216597047104	31.073745404146376	21.512444897880613
39	21.415073758237245	26.008039936604487	31.460984170372225	21.11590213478604
40	20.247422151212408	26.451744338999145	31.0679705095382	22.232863000250244
41	20.66594159656586	26.042047649297068	31.147696137878818	22.144314616258253
42	19.202005813393903	27.090511848158773	31.325755388297498	22.381726950149826
43	19.46829262032635	26.72492893671357	31.126681938054627	22.680096504905453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	60.0
1	78.0
2	96.0
3	293.0
4	490.0
5	490.0
6	789.0
7	1088.0
8	1182.5
9	1277.0
10	1901.0
11	2525.0
12	2525.0
13	4305.0
14	6085.0
15	9636.0
16	13187.0
17	13025.0
18	12863.0
19	12863.0
20	14028.0
21	15193.0
22	13048.0
23	10903.0
24	12279.0
25	13655.0
26	13655.0
27	15293.5
28	16932.0
29	18238.0
30	19544.0
31	21125.5
32	22707.0
33	22707.0
34	24531.5
35	26356.0
36	28341.0
37	30326.0
38	31767.0
39	33208.0
40	33208.0
41	34498.0
42	35788.0
43	38786.0
44	41784.0
45	47758.5
46	53733.0
47	53733.0
48	54402.0
49	55071.0
50	50504.5
51	45938.0
52	43387.5
53	40837.0
54	40837.0
55	36748.5
56	32660.0
57	28709.0
58	24758.0
59	21993.0
60	19228.0
61	19228.0
62	16991.5
63	14755.0
64	12927.5
65	11100.0
66	9332.0
67	7564.0
68	7564.0
69	6404.0
70	5244.0
71	4448.5
72	3653.0
73	2840.5
74	2028.0
75	2028.0
76	1597.5
77	1167.0
78	917.0
79	667.0
80	548.5
81	430.0
82	430.0
83	313.0
84	196.0
85	157.5
86	119.0
87	112.5
88	106.0
89	106.0
90	81.5
91	57.0
92	31.5
93	6.0
94	5.0
95	4.0
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	623388.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.46599746310948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.53057532939712	51.957561287519084
2	9.357270805403022	11.50307967173061
3	2.489013190110748	4.589690352869798
4	1.0374901151578835	2.550814591451825
5	0.5781608336819546	1.7768616168182143
6	0.3784419081079347	1.3956785618217975
7	0.2565143500004931	1.103683727046704
8	0.19978582558839764	0.9824028039025345
9	0.13763724196772742	0.7614009329055949
>10	0.9283028400731225	10.617886091105886
>50	0.06937257358006947	2.8916721516807167
>100	0.031937541298208756	3.9795669910845164
>500	0.0026178312539489428	1.1905554005749366
>1k	0.002094265003159154	1.87232094081497
>5k	7.853493761846828E-4	2.826824878672859
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6136	0.984298703215333	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6025	0.9664927781734649	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5407	0.8673570873998216	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2322	0.3724807022271843	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2135	0.3424833330125059	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1330	0.213350273024184	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1302	0.208858688328938	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1262	0.2024421387643009	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1170	0.18768407476563553	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1060	0.17003856346288349	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1055	0.16923649476730385	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	980	0.15720546433360927	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	907	0.1454952613781465	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	809	0.1297747149447856	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	784	0.1257643714668874	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	744	0.11934782190225028	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	729	0.11694161581551138	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	719	0.11533747842435207	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.6041373911592781E-4	0.0
11	0.0	0.0	0.0	1.6041373911592781E-4	0.0
12	0.0	0.0	0.0	6.416549564637113E-4	0.0
13	0.0	0.0	0.0	9.624824346955668E-4	0.0
14	0.0	0.0	0.0	0.0011228961738114947	1.6041373911592781E-4
15	0.0	0.0	0.0	0.001604137391159278	1.6041373911592781E-4
16	0.0	0.0	0.0	0.002566619825854845	1.6041373911592781E-4
17	0.0	0.0	0.0	0.0038499297387822673	1.6041373911592781E-4
18	0.0	0.0	0.0	0.004010343477898195	1.6041373911592781E-4
19	0.0	0.0	0.0	0.004010343477898195	1.6041373911592781E-4
20	0.0	0.0	0.0	0.00513323965170969	1.6041373911592781E-4
21	0.0	0.0	0.0	0.0073790319993326785	1.6041373911592781E-4
22	0.0	0.0	0.0	0.012031030433694585	1.6041373911592781E-4
23	0.0	0.0	0.0	0.02037254486772283	1.6041373911592781E-4
24	0.0	0.0	0.0	0.0328848165187652	1.6041373911592781E-4
25	0.0	0.0	0.0	0.03882012486605453	1.6041373911592781E-4
26	0.0	0.0	0.0	0.04603874312627128	1.6041373911592781E-4
27	0.0	0.0	0.0	0.09993775946922302	1.6041373911592781E-4
28	0.0	0.0	0.0	0.16458449633294192	1.6041373911592781E-4
29	0.0	0.0	0.0	0.25778487875929595	1.6041373911592781E-4
30	0.0	0.0	0.0	0.4153111705711371	1.6041373911592781E-4
31	0.0	0.0	0.0	0.6745397729824764	1.6041373911592781E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACGG	30	3.597605E-4	30.833334	5
GGTATCA	1635	0.0	27.042812	1
GCTAACG	40	0.0019302014	23.125	26
TGTACTG	95	1.6730337E-7	19.473684	5
GTTAGAC	50	0.0070313592	18.5	3
ACCCGTT	60	9.2303514E-4	18.5	30
GGACCAA	50	0.0070313592	18.5	6
TATACTG	60	9.2303514E-4	18.5	5
GTATTGG	185	0.0	18.0	1
TAATACT	125	8.556526E-9	17.76	4
TAGTGTG	65	0.0015788377	17.076923	5
CGCCGGA	65	0.0015788377	17.076923	14
TTTACAC	110	7.7935147E-7	16.818182	3
GCTTTAT	110	7.7935147E-7	16.818182	1
AATACCG	135	2.2135282E-8	16.444445	5
TTGGACC	275	0.0	16.145454	4
GCCGGAA	70	0.002590709	15.857143	15
GGACCCT	245	0.0	15.857142	6
TAAGCAG	130	2.5832014E-7	15.653846	5
GTATCAA	2840	0.0	15.568663	2
>>END_MODULE