FastQCFastQC Report
Wed 25 May 2016
SRR2088416_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088416_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1498519
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT134480.8974193854065248No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT133310.8896116765953584No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT115660.7718287188884492No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48920.3264556538822664No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG31680.2114087308869624No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT20010.13353184043712493No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18500.12345522479194457No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT18330.12232077137493752No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA16020.10690555141442985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40700.022.090911
CGGTTAC603.72726E-521.58333231
TCGCTAC555.1440956E-420.18181827
TATCGCA555.1440956E-420.1818185
AGTGCGC951.6769627E-719.4736848
TCTAACG701.2195111E-418.52
TACTACG1054.799658E-717.6190475
TAGTGCG852.7240103E-517.4117647
GTACTAG1950.017.0769231
TAGACCC1107.8114863E-716.8181825
TAACGCC1655.4569682E-1116.8181824
GGGTAGA1005.8801907E-616.651
TACACCG1005.8801907E-616.655
CGACGAG1352.2206223E-816.44444324
GTATTGG2050.016.2439041
CGCGATA803.3834233E-416.187514
CGTTTAG1504.6729838E-916.03333326
CTGTGCG1751.3096724E-1015.8571439
CGACTAT700.002592986715.85714236
TTCGCTA700.002592986715.85714226
Produced by FastQC (version 0.11.5)