##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088416_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1498519
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47558422682662	31.0	31.0	34.0	30.0	34.0
2	31.677611695280472	31.0	31.0	34.0	30.0	34.0
3	31.767191473715048	31.0	31.0	34.0	30.0	34.0
4	35.5460344513483	37.0	35.0	37.0	33.0	37.0
5	35.361809893634984	37.0	35.0	37.0	33.0	37.0
6	35.38817792767392	37.0	35.0	37.0	33.0	37.0
7	35.27722371221186	37.0	35.0	37.0	32.0	37.0
8	35.3008236799133	37.0	35.0	37.0	32.0	37.0
9	36.846532476398366	39.0	37.0	39.0	32.0	39.0
10	36.69598116540398	39.0	35.0	39.0	32.0	39.0
11	36.801687532824076	39.0	37.0	39.0	32.0	39.0
12	36.67532944193567	39.0	35.0	39.0	32.0	39.0
13	36.7144153661048	39.0	35.0	39.0	32.0	39.0
14	37.75858564355874	40.0	37.0	41.0	32.0	41.0
15	37.75511288145162	40.0	37.0	41.0	32.0	41.0
16	37.716375301214065	40.0	37.0	41.0	32.0	41.0
17	37.75749189699964	39.0	37.0	41.0	32.0	41.0
18	37.72952294899164	39.0	37.0	41.0	32.0	41.0
19	37.782930346562175	40.0	37.0	41.0	32.0	41.0
20	37.73908372199485	40.0	37.0	41.0	32.0	41.0
21	37.69062387597354	40.0	37.0	41.0	32.0	41.0
22	37.58911365154529	39.0	36.0	41.0	32.0	41.0
23	37.48933046561305	39.0	36.0	41.0	32.0	41.0
24	37.40699984451315	39.0	36.0	41.0	32.0	41.0
25	37.34102003378002	39.0	36.0	41.0	31.0	41.0
26	37.14063552080421	39.0	36.0	41.0	31.0	41.0
27	36.97824919136828	39.0	36.0	41.0	31.0	41.0
28	36.875382294118396	39.0	35.0	40.0	31.0	41.0
29	36.7362622696142	39.0	35.0	40.0	30.0	41.0
30	36.56021445173535	38.0	35.0	40.0	30.0	41.0
31	36.36652721787311	38.0	35.0	40.0	30.0	41.0
32	36.191761332355476	38.0	35.0	40.0	30.0	41.0
33	36.293701981756655	38.0	35.0	40.0	30.0	41.0
34	36.27791439414515	38.0	35.0	40.0	30.0	41.0
35	36.218009247797326	38.0	35.0	40.0	30.0	41.0
36	36.13991280724502	38.0	35.0	40.0	30.0	41.0
37	36.015634770062974	38.0	35.0	40.0	29.0	41.0
38	35.89922783761834	38.0	35.0	40.0	29.0	41.0
39	35.85161949898533	38.0	35.0	40.0	28.0	41.0
40	35.73103977994273	38.0	35.0	40.0	27.0	41.0
41	35.61210168172709	38.0	35.0	40.0	27.0	41.0
42	35.478599203613705	38.0	35.0	40.0	26.0	41.0
43	35.13939963390521	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	2.0
15	3.0
16	9.0
17	33.0
18	82.0
19	206.0
20	408.0
21	804.0
22	1532.0
23	2570.0
24	4375.0
25	6922.0
26	10722.0
27	15604.0
28	22335.0
29	30359.0
30	39321.0
31	48978.0
32	61590.0
33	75842.0
34	94591.0
35	117285.0
36	149327.0
37	204293.0
38	278205.0
39	333120.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.317660970598304	21.950272235453806	13.162328939439542	23.56973785450835
2	18.05302435271091	23.290128453493082	35.22751463278077	23.429332561015244
3	19.500586912811915	26.097900660585548	31.521255319418707	22.880257107183827
4	13.507736638641218	16.794581850480377	36.3356086909809	33.36207281989751
5	12.665171412574683	39.24254547323057	34.40803887037802	13.684244243816728
6	31.153492214646594	37.30990397852813	16.29295324250143	15.243650564323843
7	25.49570609381663	33.87337764819799	22.707019397151456	17.923896860833928
8	26.933992828919756	33.71662287898919	20.726797591488662	18.622586700602394
9	26.07741376652548	13.896720695566756	22.5598073831563	37.46605815475146
10	16.338731774505362	27.83615022565613	33.99796732640694	21.827150673431568
11	32.64236222563744	23.059834409840647	23.68698695178373	20.610816412738174
12	20.156768115719586	27.739454754994764	31.385321107039683	20.718456022245967
13	29.600892614641523	21.51237321648908	27.412798903450675	21.473935265418724
14	21.141540414235653	22.68853447970963	28.304546021772165	27.865379084282548
15	23.516151613693253	29.22298616167029	25.449860829258753	21.8110013953777
16	22.202988417230614	28.148592043210662	26.99171648807923	22.656703051479493
17	20.519859941715787	27.843090411266058	28.09527273261133	23.541776914406825
18	20.929330892701394	27.01941049796499	30.53508163726986	21.51617697206375
19	21.10597196298479	26.446444789822486	31.654520229640067	20.79306301755266
20	22.994369774423948	25.782389145549704	30.567113263161826	20.656127816864515
21	22.784095496953995	26.3832490612398	29.877365585621536	20.955289856184674
22	22.410526659988964	26.134069704821894	30.357439578677347	21.097964056511795
23	21.565692527088412	26.82702054495138	30.679157221229758	20.928129706730445
24	20.8682038732909	27.0483056938217	30.1105958616474	21.972894571240005
25	21.531859122240025	27.183038720229774	30.473887885305423	20.811214272224777
26	21.978833768540806	27.069259715759358	29.636594531000277	21.31531198469956
27	21.435897709672016	27.478597201637083	29.654278657794798	21.431226430896107
28	21.0697361861945	27.62467476221523	29.858413540302127	21.447175511288144
29	20.440848597848944	27.373159766409366	30.562975844817448	21.62301579092424
30	20.452393329680838	27.765747381247753	31.04892230262012	20.73293698645129
31	21.281078184527523	27.434086588158042	30.245862748487006	21.03897247882743
32	21.354217063647507	27.577695044240347	29.74083078025704	21.327257111855104
33	21.69942456518736	27.425144425929872	29.522014735882564	21.35341627300021
34	21.32959275124306	28.049227270391636	29.694918783145223	20.926261195220082
35	20.72799877745961	28.18816444769803	30.00862851922465	21.075208255617714
36	20.85579161825776	27.204860265368673	30.437051515529667	21.502296600843902
37	20.91998833514957	26.73019160918213	30.35049939306742	21.999320662600873
38	20.81101407456295	26.889348750332832	30.681359395509833	21.618277779594386
39	21.237234896587896	26.37036967832907	30.955363261994005	21.43703216308902
40	20.4582657944277	26.471869892874235	30.96897670299809	22.100887609699978
41	20.493300385247036	26.147216017948388	30.99860595694816	22.36087763985642
42	19.509796005255854	26.955280513627123	30.937545670091605	22.597377811025417
43	19.692976865825525	26.44624459216066	30.921730054807444	22.939048487206367
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	257.0
1	292.0
2	327.0
3	892.0
4	1457.0
5	1457.0
6	2229.0
7	3001.0
8	3154.5
9	3308.0
10	4785.0
11	6262.0
12	6262.0
13	10703.5
14	15145.0
15	23145.0
16	31145.0
17	30314.0
18	29483.0
19	29483.0
20	32202.0
21	34921.0
22	30522.0
23	26123.0
24	30003.5
25	33884.0
26	33884.0
27	37218.5
28	40553.0
29	43295.0
30	46037.0
31	50129.0
32	54221.0
33	54221.0
34	58819.0
35	63417.0
36	69312.0
37	75207.0
38	78052.0
39	80897.0
40	80897.0
41	85219.5
42	89542.0
43	94692.0
44	99842.0
45	109334.5
46	118827.0
47	118827.0
48	120318.5
49	121810.0
50	113021.0
51	104232.0
52	98254.5
53	92277.0
54	92277.0
55	85556.0
56	78835.0
57	73092.0
58	67349.0
59	60442.5
60	53536.0
61	53536.0
62	47356.5
63	41177.0
64	35429.5
65	29682.0
66	24751.5
67	19821.0
68	19821.0
69	16874.5
70	13928.0
71	11649.5
72	9371.0
73	7493.5
74	5616.0
75	5616.0
76	4306.5
77	2997.0
78	2374.0
79	1751.0
80	1369.0
81	987.0
82	987.0
83	765.5
84	544.0
85	437.5
86	331.0
87	276.5
88	222.0
89	222.0
90	182.0
91	142.0
92	88.5
93	35.0
94	26.0
95	17.0
96	17.0
97	10.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1498519.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.656118762703905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.73427047773714	37.60669929249256
2	13.234212294793354	13.1431923488219
3	4.666629600732866	6.95180141026622
4	2.142977664968411	4.256478137499734
5	1.1115684736718583	2.7598088070763653
6	0.65114049752607	1.9399865925816384
7	0.4194326600163329	1.4579178585109485
8	0.2937161974863141	1.1667845107928165
9	0.22114789947393249	0.9883211724360087
>10	1.2930374280653516	12.129690788654134
>50	0.1434122483884697	4.975188193099249
>100	0.08265652618696873	7.198677413550528
>500	0.003370948228353059	1.0676689304078528
>1k	0.002022568937011835	1.7903842467769961
>5k	0.0	0.0
>10k+	4.0451378740236706E-4	2.567400297033056
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13448	0.8974193854065248	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13331	0.8896116765953584	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11566	0.7718287188884492	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4892	0.3264556538822664	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3168	0.2114087308869624	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2001	0.13353184043712493	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1850	0.12345522479194457	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1833	0.12232077137493752	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1602	0.10690555141442985	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	1.3346510788318333E-4	0.0	0.0	0.0	0.0
7	1.3346510788318333E-4	0.0	0.0	0.0	0.0
8	1.3346510788318333E-4	0.0	0.0	0.0	0.0
9	1.3346510788318333E-4	0.0	0.0	0.0	0.0
10	1.3346510788318333E-4	0.0	0.0	6.673255394159166E-5	0.0
11	1.3346510788318333E-4	0.0	0.0	6.673255394159166E-5	0.0
12	1.3346510788318333E-4	0.0	0.0	2.00197661824775E-4	0.0
13	1.3346510788318333E-4	0.0	0.0	4.6712787759114165E-4	0.0
14	1.3346510788318333E-4	0.0	0.0	5.338604315327333E-4	0.0
15	1.3346510788318333E-4	0.0	0.0	6.673255394159166E-4	0.0
16	1.3346510788318333E-4	0.0	0.0	0.0011344534170070583	0.0
17	1.3346510788318333E-4	0.0	0.0	0.0016015812945982	0.0
18	1.3346510788318333E-4	0.0	0.0	0.0017350464024813834	0.0
19	1.3346510788318333E-4	0.0	0.0	0.0022689068340141167	0.0
20	1.3346510788318333E-4	0.0	0.0	0.0028694998194884417	0.0
21	1.3346510788318333E-4	0.0	0.0	0.0036035579128459497	0.0
22	1.3346510788318333E-4	0.0	0.0	0.0060726624086848415	0.0
23	1.3346510788318333E-4	0.0	0.0	0.009876417983355566	0.0
24	1.3346510788318333E-4	0.0	0.0	0.015882347838098818	0.0
25	1.3346510788318333E-4	0.0	0.0	0.02235540557043321	0.0
26	1.3346510788318333E-4	0.0	0.0	0.031898160784080816	0.0
27	1.3346510788318333E-4	0.0	0.0	0.09662873810742473	0.0
28	1.3346510788318333E-4	0.0	0.0	0.16683138485397916	0.0
29	1.3346510788318333E-4	0.0	0.0	0.24570926361294051	0.0
30	1.3346510788318333E-4	0.0	0.0	0.3763048716766354	0.0
31	1.3346510788318333E-4	0.0	0.0	0.6069325780987762	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4070	0.0	22.09091	1
CGGTTAC	60	3.72726E-5	21.583332	31
TCGCTAC	55	5.1440956E-4	20.181818	27
TATCGCA	55	5.1440956E-4	20.181818	5
AGTGCGC	95	1.6769627E-7	19.473684	8
TCTAACG	70	1.2195111E-4	18.5	2
TACTACG	105	4.799658E-7	17.619047	5
TAGTGCG	85	2.7240103E-5	17.411764	7
GTACTAG	195	0.0	17.076923	1
TAGACCC	110	7.8114863E-7	16.818182	5
TAACGCC	165	5.4569682E-11	16.818182	4
GGGTAGA	100	5.8801907E-6	16.65	1
TACACCG	100	5.8801907E-6	16.65	5
CGACGAG	135	2.2206223E-8	16.444443	24
GTATTGG	205	0.0	16.243904	1
CGCGATA	80	3.3834233E-4	16.1875	14
CGTTTAG	150	4.6729838E-9	16.033333	26
CTGTGCG	175	1.3096724E-10	15.857143	9
CGACTAT	70	0.0025929867	15.857142	36
TTCGCTA	70	0.0025929867	15.857142	26
>>END_MODULE