FastQCFastQC Report
Wed 25 May 2016
SRR2088415_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088415_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208086
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT246131.1146757870843798No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT231841.0499591048537058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT205040.9285870206142334No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG83070.3762081730512308No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80660.3652937430879051No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT50290.22775381031354758No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT49840.22571584621251164No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA45190.20465688383514047No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41470.18780971393324353No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA39310.1780274862482711No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT38620.17490260796001605No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT34640.1568779476886317No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG33600.152167986210682No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32500.14718629618592755No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27550.12476869107453242No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT26500.12001344150544861No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA25920.11738673221966897No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA24910.11281263501512169No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22650.10257752641880796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56350.027.4791491
GTATTGG5600.020.1517871
ATTGGAC5200.019.5673083
ACGTTTA1601.8189894E-1218.526
ATAGACG500.007036885218.4999983
ATCACGT1750.017.9714323
CGAATTA1851.8189894E-1217.015
GTATCAA92250.016.8254742
CCGTATA1107.8156154E-716.8181822
CGACGAG1452.9831426E-916.58620624
TGCGACG1352.2222594E-816.44444522
CCCGTAT1059.350815E-615.8571441
TATACTC2700.015.7592595
CGTATAC1406.0015554E-714.5357153
CCGAATT2201.8189894E-1114.29545414
CTGTGCG2201.8189894E-1114.2954549
TCACGTT2201.8189894E-1114.29545424
CGAACGA1304.4481403E-614.23076916
GGACCCT9800.014.1581646
TAGACTG2101.364242E-1014.0952395
Produced by FastQC (version 0.11.5)