FastQCFastQC Report
Wed 25 May 2016
SRR2088414_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088414_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2955766
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT348691.179694197713892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT336121.137167150579579No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT304441.0299868122172051No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG144680.4894839442635175No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117990.3991858624803181No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA81060.2742436309234222No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA79720.2697101191366299No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT77970.2637894880717892No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT75520.25550060458101215No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA62580.2117217668787042No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT60990.2063424506540775No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG56880.19243742569608013No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT55420.1874979277791273No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47010.15904506649037845No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA45630.15437622599353265No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44690.15119600130727534No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA43330.14659482516545624No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT39040.13208082101221816No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA31790.10755249231502088No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC31050.10504891117903109No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30280.10244383351050118No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC29660.10034623850467188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85850.028.1001761
ATTGGAC8950.018.3966483
GTATTGG9000.017.4722231
GTATCAA141600.017.0367242
TATACTG3800.016.0657885
CGAACGA2700.015.75925916
TAGTACT4700.015.7446814
CAATGCG1701.4897523E-915.23529419
TATACCG2201.8189894E-1215.1363645
ACGTTTA1601.0984877E-815.0312526
TTGGACC16050.014.869164
GGACCCT16150.014.5479886
CGTCTGT7150.014.23076934
TAACGAA3050.013.9508213
AGCCGTC2009.858923E-1013.87499912
TGGACCC16850.013.8338295
CTAGTAC4150.013.8192773
TGCGACG1753.5719495E-813.74285822
TAAACGT1907.1395334E-913.63157754
CGGCTAA950.001245884513.631577526
Produced by FastQC (version 0.11.5)