##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088414_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2955766
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.375154866792567	31.0	31.0	34.0	30.0	34.0
2	31.569926374415296	31.0	31.0	34.0	30.0	34.0
3	31.64493231196245	31.0	31.0	34.0	30.0	34.0
4	35.43961971279188	37.0	35.0	37.0	33.0	37.0
5	35.269339995114635	37.0	35.0	37.0	33.0	37.0
6	35.31579360477115	37.0	35.0	37.0	32.0	37.0
7	35.19992719315399	37.0	35.0	37.0	32.0	37.0
8	35.235721298641366	37.0	35.0	37.0	32.0	37.0
9	36.73798094977749	39.0	37.0	39.0	32.0	39.0
10	36.59496827556715	39.0	35.0	39.0	32.0	39.0
11	36.709133266977155	39.0	35.0	39.0	32.0	39.0
12	36.57151242689712	39.0	35.0	39.0	32.0	39.0
13	36.611298729331075	39.0	35.0	39.0	32.0	39.0
14	37.61823906222617	39.0	36.0	41.0	32.0	41.0
15	37.608816800788695	39.0	36.0	41.0	32.0	41.0
16	37.559490839261294	39.0	36.0	41.0	32.0	41.0
17	37.62324588617638	39.0	36.0	41.0	32.0	41.0
18	37.59866308767338	39.0	36.0	41.0	32.0	41.0
19	37.66687281740165	39.0	37.0	41.0	32.0	41.0
20	37.62571259023888	39.0	37.0	41.0	32.0	41.0
21	37.584282720621324	39.0	36.0	41.0	32.0	41.0
22	37.469095659128634	39.0	36.0	41.0	32.0	41.0
23	37.35979302827084	39.0	36.0	41.0	32.0	41.0
24	37.28755219459186	39.0	36.0	41.0	31.0	41.0
25	37.226932713888715	39.0	36.0	41.0	31.0	41.0
26	37.00161074997141	39.0	36.0	40.0	31.0	41.0
27	36.828491835957244	39.0	35.0	40.0	31.0	41.0
28	36.70704040847618	39.0	35.0	40.0	30.0	41.0
29	36.55721291874932	38.0	35.0	40.0	30.0	41.0
30	36.37563020888663	38.0	35.0	40.0	30.0	41.0
31	36.14112010220024	38.0	35.0	40.0	30.0	41.0
32	35.99617358072324	38.0	35.0	40.0	30.0	41.0
33	36.05611404962369	38.0	35.0	40.0	30.0	41.0
34	36.028777988514655	38.0	35.0	40.0	30.0	41.0
35	35.94154138047464	38.0	35.0	40.0	29.0	41.0
36	35.85687567960387	38.0	35.0	40.0	29.0	41.0
37	35.72173067827426	38.0	35.0	40.0	28.0	41.0
38	35.60563014798871	38.0	35.0	40.0	27.0	41.0
39	35.53221872096776	38.0	34.0	40.0	27.0	41.0
40	35.370270177003185	38.0	34.0	40.0	26.0	41.0
41	35.228556996731136	38.0	34.0	40.0	25.0	41.0
42	35.057913244823844	38.0	34.0	40.0	24.0	41.0
43	34.7167695277637	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	4.0
15	21.0
16	31.0
17	78.0
18	200.0
19	384.0
20	946.0
21	1839.0
22	3419.0
23	6023.0
24	9656.0
25	15840.0
26	23554.0
27	34241.0
28	48682.0
29	65678.0
30	84961.0
31	103711.0
32	126518.0
33	156009.0
34	191955.0
35	233877.0
36	299672.0
37	408733.0
38	546102.0
39	593628.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.332094624540645	22.37792166226961	13.660655139818239	22.629328573371506
2	18.325063621409814	23.111200277694515	35.62183203947809	22.94190406141758
3	20.12178907261265	26.0768951263395	31.83452275992078	21.966793041127072
4	13.271720427124475	16.150060593429927	35.59730371078089	34.9809152686647
5	11.9188054805421	39.99501313703453	34.354647830714605	13.731533551708761
6	31.458748764279715	36.50045368950045	15.80551369763371	16.235283848586118
7	25.06923755128112	34.67544453789644	21.813634773524022	18.44168313729842
8	27.647215645622826	33.33423552473369	20.35391164253192	18.664637187111563
9	25.799978753392523	13.62218795398553	23.034062912964018	37.54377037965793
10	16.521910056479435	27.430520548649657	33.589431639717084	22.458137755153825
11	32.14740273756448	22.148235009131305	24.476294808181702	21.228067445122516
12	20.452803097403518	27.34898500084242	31.413379814234283	20.784832087519785
13	30.642818139189636	20.889779502166274	28.245165550994226	20.222236807649864
14	21.19379544930147	22.42650466917882	28.19698853021518	28.182711351304533
15	23.321196603520036	28.876676976458892	26.32546013453027	21.4766662854908
16	22.155001444633978	28.084598036515747	26.969996948337588	22.790403570512684
17	20.037952936734506	27.017294332501287	27.921425444368737	25.023327286395475
18	20.98515241057648	26.015895710282884	31.616575872379617	21.38237600676102
19	20.5010816147151	25.008407296112072	32.706648631860574	21.78386245731225
20	23.958628660049545	24.193965286832583	31.541671431365	20.305734621752872
21	23.657759105423096	25.008407296112072	30.559895472104355	20.773938126360477
22	22.3943302683636	24.548357346285194	31.411383715760994	21.645928669590216
23	21.359471622584465	26.01687684343077	32.219938926153155	20.403712607831608
24	20.31683157597726	25.56826216960341	30.952010409484377	23.16289584493495
25	21.36965510801599	26.193413145695565	31.706907786340327	20.730023959948117
26	22.377549508316964	25.549992793746192	30.64826511976929	21.424192578167556
27	21.36349765170856	26.58853914687428	30.332238749616852	21.715724451800313
28	21.086581278761578	26.12791405002967	30.686393983826864	22.099110687381884
29	20.225687689756224	26.230087226120062	31.413921129074495	22.130303955049214
30	19.83076468164259	26.538095370201837	32.62836097309462	21.00277897506095
31	20.981261710162443	26.067557445345805	31.730996296729852	21.2201845477619
32	21.732708204912026	26.119456005651326	30.23571554717119	21.912120242265456
33	22.310121978532806	26.013155303904302	29.975343109028252	21.70137960853464
34	21.330680439520584	27.93851069401299	30.040909869049177	20.689898997417252
35	20.216654498360153	28.634235592398046	30.3579173723495	20.791192536892297
36	20.799379923850537	26.06542601816247	31.71035866844669	21.42483538954031
37	21.04588116921299	26.052434461997333	31.09616931786887	21.80551505092081
38	20.965225258021103	25.701899270781247	31.646889503431595	21.685985967766054
39	21.901869092478904	25.32548246376743	31.54769355896238	21.224954884791288
40	20.46224227492975	25.88334123878548	31.12841138303912	22.526005103245655
41	21.049941030514592	25.531283599581293	31.03046722913789	22.388308140766217
42	19.295099815073318	26.917827730611965	31.348997180426323	22.438075273888394
43	19.697973384902596	26.403984618538818	31.01926877838097	22.878773218177624
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	274.0
1	407.0
2	540.0
3	1445.0
4	2350.0
5	2350.0
6	3966.0
7	5582.0
8	5883.0
9	6184.0
10	9364.5
11	12545.0
12	12545.0
13	22226.5
14	31908.0
15	51371.5
16	70835.0
17	68956.0
18	67077.0
19	67077.0
20	71911.0
21	76745.0
22	62679.5
23	48614.0
24	54289.0
25	59964.0
26	59964.0
27	65224.5
28	70485.0
29	74531.0
30	78577.0
31	84432.5
32	90288.0
33	90288.0
34	98456.0
35	106624.0
36	117323.5
37	128023.0
38	134234.5
39	140446.0
40	140446.0
41	148975.5
42	157505.0
43	175854.5
44	194204.0
45	233173.5
46	272143.0
47	272143.0
48	276857.0
49	281571.0
50	258537.0
51	235503.0
52	223012.5
53	210522.0
54	210522.0
55	186168.5
56	161815.0
57	139944.5
58	118074.0
59	105144.5
60	92215.0
61	92215.0
62	82391.5
63	72568.0
64	64190.5
65	55813.0
66	47749.0
67	39685.0
68	39685.0
69	33020.5
70	26356.0
71	22108.5
72	17861.0
73	13855.0
74	9849.0
75	9849.0
76	7498.5
77	5148.0
78	4159.5
79	3171.0
80	2640.0
81	2109.0
82	2109.0
83	1599.5
84	1090.0
85	870.5
86	651.0
87	550.0
88	449.0
89	449.0
90	372.5
91	296.0
92	176.5
93	57.0
94	46.0
95	35.0
96	35.0
97	24.0
98	13.0
99	7.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2955766.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.88105785704708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.70596006019208	30.868835776901065
2	13.702764887603564	11.477725781184764
3	5.08121839507075	6.3842040476475
4	2.4359224962096353	4.080760439961531
5	1.305819989448167	2.734456126448365
6	0.7803954681486165	1.9610272651745728
7	0.5214875861182072	1.528831623616392
8	0.3562879432223788	1.19373727710918
9	0.2726107265406947	1.0275503049632226
>10	1.5514308895238869	12.41608186377188
>50	0.16220847420516066	4.781364809156111
>100	0.10885915271295239	8.728961779679455
>500	0.008244413776468111	2.230641170931824
>1k	0.0057387586091018445	4.485587681576622
>5k	6.466206883495036E-4	1.862303564420686
>10k+	4.041379302184397E-4	4.237930487456911
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34869	1.179694197713892	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33612	1.137167150579579	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30444	1.0299868122172051	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14468	0.4894839442635175	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11799	0.3991858624803181	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8106	0.2742436309234222	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7972	0.2697101191366299	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7797	0.2637894880717892	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	7552	0.25550060458101215	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6258	0.2117217668787042	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6099	0.2063424506540775	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5688	0.19243742569608013	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5542	0.1874979277791273	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4701	0.15904506649037845	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4563	0.15437622599353265	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4469	0.15119600130727534	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4333	0.14659482516545624	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3904	0.13208082101221816	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3179	0.10755249231502088	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3105	0.10504891117903109	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3028	0.10244383351050118	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2966	0.10034623850467188	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	6.76643550267511E-5	0.0	0.0	0.0	0.0
7	6.76643550267511E-5	0.0	0.0	0.0	0.0
8	1.0149653254012665E-4	0.0	0.0	0.0	0.0
9	1.0149653254012665E-4	0.0	0.0	2.3682524259362886E-4	0.0
10	1.0149653254012665E-4	0.0	0.0	3.0448959762037996E-4	0.0
11	1.0149653254012665E-4	0.0	0.0	5.751470177273844E-4	0.0
12	1.0149653254012665E-4	0.0	0.0	9.81133147887891E-4	0.0
13	1.0149653254012665E-4	0.0	0.0	0.0010487975029146422	0.0
14	1.0149653254012665E-4	0.0	0.0	0.0012517905679948955	0.0
15	1.0149653254012665E-4	0.0	0.0	0.0017254410531821532	0.0
16	1.0149653254012665E-4	0.0	0.0	0.002875735088636922	0.0
17	1.0149653254012665E-4	0.0	0.0	0.003958364769064939	0.0
18	1.0149653254012665E-4	0.0	0.0	0.004398183076738822	0.0
19	1.0149653254012665E-4	0.0	0.0	0.005007162271979582	0.0
20	1.0149653254012665E-4	0.0	0.0	0.005954463242354097	0.0
21	1.0149653254012665E-4	0.0	0.0	0.008627205265910766	0.0
22	1.0149653254012665E-4	0.0	0.0	0.014581668508264863	0.0
23	1.0149653254012665E-4	0.0	0.0	0.02358102772682276	0.0
24	1.0149653254012665E-4	0.0	0.0	0.036166597761798466	0.0
25	1.353287100535022E-4	0.0	0.0	0.04242555060177294	0.0
26	1.353287100535022E-4	0.0	0.0	0.052304546435678605	0.0
27	1.353287100535022E-4	0.0	0.0	0.12118685985291122	0.0
28	1.353287100535022E-4	0.0	0.0	0.19368921626407504	0.0
29	1.353287100535022E-4	0.0	0.0	0.2788448070652413	0.0
30	1.353287100535022E-4	0.0	0.0	0.429533325709816	0.0
31	1.353287100535022E-4	0.0	0.0	0.6675088623388996	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8585	0.0	28.100176	1
ATTGGAC	895	0.0	18.396648	3
GTATTGG	900	0.0	17.472223	1
GTATCAA	14160	0.0	17.036724	2
TATACTG	380	0.0	16.065788	5
CGAACGA	270	0.0	15.759259	16
TAGTACT	470	0.0	15.744681	4
CAATGCG	170	1.4897523E-9	15.235294	19
TATACCG	220	1.8189894E-12	15.136364	5
ACGTTTA	160	1.0984877E-8	15.03125	26
TTGGACC	1605	0.0	14.86916	4
GGACCCT	1615	0.0	14.547988	6
CGTCTGT	715	0.0	14.230769	34
TAACGAA	305	0.0	13.95082	13
AGCCGTC	200	9.858923E-10	13.874999	12
TGGACCC	1685	0.0	13.833829	5
CTAGTAC	415	0.0	13.819277	3
TGCGACG	175	3.5719495E-8	13.742858	22
TAAACGT	190	7.1395334E-9	13.6315775	4
CGGCTAA	95	0.0012458845	13.6315775	26
>>END_MODULE