FastQCFastQC Report
Wed 25 May 2016
SRR2088413_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088413_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2995448
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT280460.9362873266369506No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT272460.9095801362600853No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT251640.8400746733042938No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG119130.39770344869949337No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95980.3204195165464398No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA64650.2158274822330416No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT63460.2118547876644829No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA61830.20641319762519664No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT59690.19926902419938522No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA52510.17529932083614871No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT50720.16932358698932515No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT43870.14645555522913434No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG43070.14378483619144783No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37330.12462242709604707No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA36510.12188494008241839No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35800.11951467693647161No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA33310.11120206393167233No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT30880.10308975485469954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC454.0110863E-628.7777773
GGTATCA69150.026.9407081
ACGTTTA1700.020.6764726
GACGCGA851.2466753E-619.58823433
GTATTGG10200.019.2254921
ATTGGAC9550.017.2408373
AACGGTA1554.0199666E-1016.70967918
GGACCCT13350.016.2134826
CGACGGT3500.015.8571427
TGGACCC14050.015.8007125
GTATCAA118900.015.6526492
GTTATAC1802.0190782E-1015.4166663
TACGACG3500.015.3285715
TTGGACC15000.015.2933324
GAACCGT855.3677254E-415.2352936
CGTTTAG1252.9624025E-614.80000126
ACGACGG3800.014.6052646
TACTTAC1152.212077E-514.4782617
ACCGTTA1152.212077E-514.4782618
TCACGTT2301.8189894E-1214.47826124
Produced by FastQC (version 0.11.5)