FastQCFastQC Report
Wed 25 May 2016
SRR2088412_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088412_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960825
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT286070.9661834117180178No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT269210.9092398233600432No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT252080.8513843270034535No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG124160.41934258188173906No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96840.3270710021700033No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA67320.2273690609880692No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA67210.22699754291455929No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT64060.21635861626404804No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT60880.20561836650257953No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA49900.168534107892226No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT49880.16846655915158784No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG46650.1575574375385239No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT40070.13533390186856706No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA38980.13165249550378694No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA38510.13006510009879005No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT34710.11723083937753835No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA33150.11196203760776136No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT30160.10186350088235542No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75550.025.7359351
ACCGTTA1258.594725E-917.768
ACGTTTA1705.456968E-1217.41176626
GTATTGG11150.016.9237671
TCTAACG1005.8840888E-616.652
CCCGTAT904.4497647E-516.4444451
ATTGGAC10700.016.4252343
CGAACGA2300.016.08695616
TTACGGC1403.478999E-815.85714318
CGCGATA1302.5927875E-715.65384614
GTATCAA124750.015.6008012
CGAGCCG4500.014.815
GACCGTG2450.014.346947
TTGGACC16950.014.29793554
TGGACCC16400.013.8750015
AAGACCG2551.8189894E-1213.7843135
GGACCCT16000.013.7593746
CGCGTAT1102.45841E-413.45454524
TAGTACG1102.45841E-413.4545454
ACCGTCG4000.013.4124998
Produced by FastQC (version 0.11.5)