##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088412_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2960825
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.526757913757145	31.0	31.0	34.0	30.0	34.0
2	31.722947827041448	31.0	31.0	34.0	30.0	34.0
3	31.810952015063368	31.0	31.0	34.0	30.0	34.0
4	35.5793810846639	37.0	35.0	37.0	33.0	37.0
5	35.41296800722772	37.0	35.0	37.0	33.0	37.0
6	35.44995904857599	37.0	35.0	37.0	33.0	37.0
7	35.34318272778702	37.0	35.0	37.0	32.0	37.0
8	35.368779647564445	37.0	35.0	37.0	32.0	37.0
9	36.919473119823024	39.0	37.0	39.0	33.0	39.0
10	36.78337355297932	39.0	37.0	39.0	32.0	39.0
11	36.88075553266404	39.0	37.0	39.0	33.0	39.0
12	36.751315596159856	39.0	37.0	39.0	32.0	39.0
13	36.79287833627452	39.0	37.0	39.0	32.0	39.0
14	37.85574696241757	40.0	37.0	41.0	32.0	41.0
15	37.84211191137605	40.0	37.0	41.0	32.0	41.0
16	37.79860140332509	40.0	37.0	41.0	32.0	41.0
17	37.84033740595949	40.0	37.0	41.0	32.0	41.0
18	37.81030658684657	40.0	37.0	41.0	32.0	41.0
19	37.857982150245284	40.0	37.0	41.0	32.0	41.0
20	37.822913546055574	40.0	37.0	41.0	32.0	41.0
21	37.776608884348114	40.0	37.0	41.0	32.0	41.0
22	37.66781049200814	39.0	37.0	41.0	32.0	41.0
23	37.57177644744286	39.0	36.0	41.0	32.0	41.0
24	37.49679430564117	39.0	36.0	41.0	32.0	41.0
25	37.44035868381279	39.0	36.0	41.0	32.0	41.0
26	37.241964655121464	39.0	36.0	41.0	31.0	41.0
27	37.07994224582676	39.0	36.0	41.0	31.0	41.0
28	36.965388025297	39.0	35.0	40.0	31.0	41.0
29	36.82434828130673	39.0	35.0	40.0	30.0	41.0
30	36.65591887396249	39.0	35.0	40.0	30.0	41.0
31	36.46214585461822	38.0	35.0	40.0	30.0	41.0
32	36.324970574079856	38.0	35.0	40.0	30.0	41.0
33	36.407903540398365	38.0	35.0	40.0	30.0	41.0
34	36.388165460640195	38.0	35.0	40.0	30.0	41.0
35	36.310814722248026	38.0	35.0	40.0	30.0	41.0
36	36.235168238582155	38.0	35.0	40.0	30.0	41.0
37	36.1169937432979	38.0	35.0	40.0	30.0	41.0
38	36.00737058083473	38.0	35.0	40.0	29.0	41.0
39	35.95799211368453	38.0	35.0	40.0	28.0	41.0
40	35.819966394501535	38.0	35.0	40.0	27.0	41.0
41	35.702295137335035	38.0	35.0	40.0	27.0	41.0
42	35.558360929808416	38.0	35.0	40.0	26.0	41.0
43	35.23099710384775	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	3.0
15	10.0
16	35.0
17	80.0
18	177.0
19	374.0
20	775.0
21	1544.0
22	2888.0
23	4954.0
24	8173.0
25	12975.0
26	19995.0
27	29674.0
28	41708.0
29	57860.0
30	74607.0
31	94208.0
32	116530.0
33	145397.0
34	182412.0
35	227249.0
36	293339.0
37	403758.0
38	555217.0
39	686880.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.827767598557834	21.652309744750195	13.150760345511808	23.369162311180162
2	18.17354960188461	22.641392179544553	35.934950562765444	23.25010765580539
3	19.776650089079904	25.940776641645485	31.335286617750118	22.94728665152449
4	13.171058742073576	15.991961699864058	35.881924801364484	34.955054756697876
5	12.453589793385289	39.216907449781736	34.49852659309483	13.830976163738148
6	31.71977404946257	36.493544873472764	15.977641366848767	15.809039710215902
7	25.743365447130444	33.78443508143845	22.091511656379556	18.380687815051548
8	27.26199623415771	33.526162471608416	20.41417510322292	18.79766619101095
9	25.988736247498583	13.714859878581139	22.48120034112114	37.81520353279914
10	16.660086293516166	26.897368132192884	33.672067751386855	22.77047782290409
11	32.87208801600905	22.092693759340722	24.089873599419082	20.945344625231144
12	20.597738805907138	26.776861178894396	31.26094922867782	21.36445078652065
13	30.365455574037643	20.718313307946264	27.736424814029874	21.179806303986222
14	21.423961227022872	22.14656387999966	27.489095100183224	28.94037979279424
15	23.570930399466363	28.789273259986658	25.445847018989642	22.193949321557334
16	22.768181165722392	27.826636157152144	26.307904047013924	23.09727863011154
17	20.768907314684252	26.828975032296743	27.40790151393615	24.994216139082855
18	21.73286161796121	25.90021362289227	30.493460437546965	21.873464321599553
19	21.234554558273455	25.053490158992847	31.311577009786124	22.400378272947574
20	24.209705065311187	24.28893973807976	30.33313350164228	21.168221694966775
21	23.999898676889043	25.181495022502176	29.443854331140813	21.37475196946797
22	22.952724325145862	24.6728867798671	30.249406837621272	22.12498205736577
23	21.855901649033633	25.766332018947423	31.07140746244712	21.306358869571827
24	21.01988466052536	25.62022409294707	29.904671839774387	23.455219406753187
25	22.055406854508455	25.98961438112688	30.458909256710548	21.496069507654116
26	22.818133459424317	25.685138432700345	29.607491155336774	21.889236952538564
27	22.128731012471185	26.400412047317896	29.298996056842263	22.171860883368655
28	21.71364400124965	26.005251914584615	29.682976872999923	22.598127211165806
29	20.920959529860767	26.051421478810806	30.417366781218075	22.610252210110357
30	20.608039988854458	26.440130706813136	31.321810643992805	21.6300186603396
31	21.689258905879274	25.897275252674508	30.552768231827276	21.86069760961894
32	22.232688524313325	25.848809031266622	29.27673874680199	22.64176369761806
33	22.657941621000905	25.760590375993175	29.186932696123545	22.39453530688237
34	21.948004356893772	27.360448523637835	29.24576764921939	21.445779470249
35	21.19861862825395	28.052012530291385	29.48971992603413	21.259648915420534
36	21.38211478219753	26.004002262882814	30.567932924100543	22.045950030819114
37	21.741305210540983	25.865459795833935	30.1105266268692	22.282708366755887
38	21.52872932375267	25.412613038595662	30.74764634856839	22.31101128908328
39	22.273656835510373	25.090675740714158	30.677496981415654	21.958170442359815
40	21.2321565779808	25.63927283780703	30.31320662315402	22.815363961058154
41	21.45175753379548	25.430277034272542	30.386226811783878	22.7317386201481
42	20.193560916298665	26.571614330465327	30.56894615521012	22.665878598025888
43	20.337405959487644	26.077022451512672	30.331208362534092	23.254363226465596
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	272.0
1	390.0
2	508.0
3	1472.5
4	2437.0
5	2437.0
6	3804.0
7	5171.0
8	5375.5
9	5580.0
10	8170.0
11	10760.0
12	10760.0
13	18629.5
14	26499.0
15	42109.5
16	57720.0
17	57297.0
18	56874.0
19	56874.0
20	61361.5
21	65849.0
22	55018.0
23	44187.0
24	49821.5
25	55456.0
26	55456.0
27	61611.0
28	67766.0
29	72571.5
30	77377.0
31	83744.5
32	90112.0
33	90112.0
34	99206.0
35	108300.0
36	119414.5
37	130529.0
38	138276.0
39	146023.0
40	146023.0
41	154709.5
42	163396.0
43	177848.0
44	192300.0
45	224920.0
46	257540.0
47	257540.0
48	263363.0
49	269186.0
50	251035.0
51	232884.0
52	223043.5
53	213203.0
54	213203.0
55	190663.0
56	168123.0
57	148367.5
58	128612.0
59	116960.0
60	105308.0
61	105308.0
62	94207.0
63	83106.0
64	73595.5
65	64085.0
66	55024.0
67	45963.0
68	45963.0
69	39182.5
70	32402.0
71	27703.5
72	23005.0
73	17935.0
74	12865.0
75	12865.0
76	9782.5
77	6700.0
78	5351.5
79	4003.0
80	3402.5
81	2802.0
82	2802.0
83	2100.0
84	1398.0
85	1198.5
86	999.0
87	904.5
88	810.0
89	810.0
90	675.5
91	541.0
92	328.0
93	115.0
94	81.5
95	48.0
96	48.0
97	28.5
98	9.0
99	5.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2960825.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.98075701272783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.30265680561388	31.935844375621976
2	13.724466263664715	11.797758992159073
3	4.883502995417193	6.296899668508646
4	2.2677188730307005	3.8987309541963806
5	1.192093840143404	2.5618547839786627
6	0.7172425466396933	1.8496576569786454
7	0.4868130565187984	1.4646515585000468
8	0.33352697964836003	1.1468193655564145
9	0.25776529620942473	0.9971052806421987
>10	1.5487895243298169	12.627329046946167
>50	0.156372320581405	4.685046955609273
>100	0.11424957535296056	9.655768666277154
>500	0.008818166804177657	2.6290537720496436
>1k	0.005275153356061171	4.095422134130133
>5k	3.936681609000875E-4	1.2057641260365122
>10k+	3.1493452872006995E-4	3.15229266280916
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28607	0.9661834117180178	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26921	0.9092398233600432	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25208	0.8513843270034535	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12416	0.41934258188173906	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9684	0.3270710021700033	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6732	0.2273690609880692	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6721	0.22699754291455929	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	6406	0.21635861626404804	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6088	0.20561836650257953	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4990	0.168534107892226	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4988	0.16846655915158784	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4665	0.1575574375385239	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4007	0.13533390186856706	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3898	0.13165249550378694	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3851	0.13006510009879005	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3471	0.11723083937753835	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3315	0.11196203760776136	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3016	0.10186350088235542	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.377437031908337E-5
2	0.0	0.0	0.0	3.377437031908337E-5	3.377437031908337E-5
3	0.0	0.0	0.0	3.377437031908337E-5	3.377437031908337E-5
4	0.0	0.0	0.0	6.754874063816673E-5	3.377437031908337E-5
5	0.0	0.0	0.0	1.013231109572501E-4	3.377437031908337E-5
6	0.0	0.0	0.0	1.013231109572501E-4	3.377437031908337E-5
7	0.0	0.0	0.0	1.6887185159541681E-4	3.377437031908337E-5
8	0.0	0.0	0.0	2.026462219145002E-4	3.377437031908337E-5
9	0.0	0.0	0.0	3.3774370319083363E-4	3.377437031908337E-5
10	0.0	0.0	0.0	4.3906681414808375E-4	3.377437031908337E-5
11	0.0	0.0	0.0	6.079386657435005E-4	1.013231109572501E-4
12	0.0	0.0	0.0	8.781336282961675E-4	1.013231109572501E-4
13	0.0	0.0	0.0	9.119079986152508E-4	1.013231109572501E-4
14	0.0	0.0	0.0	0.0010807798502106677	1.013231109572501E-4
15	0.0	0.0	0.0	0.001486072294039668	1.013231109572501E-4
16	0.0	0.0	0.0	0.002330431552016752	1.013231109572501E-4
17	0.0	0.0	0.0	0.0033774370319083363	1.013231109572501E-4
18	0.0	0.0	0.0	0.00371518073509917	1.013231109572501E-4
19	0.0	0.0	0.0	0.00422179628988542	1.013231109572501E-4
20	0.0	0.0	0.0	0.005471447991691505	1.013231109572501E-4
21	0.0	0.0	0.0	0.0072952639889220065	1.3509748127633347E-4
22	0.0	0.0	0.0	0.011989901463274595	1.3509748127633347E-4
23	0.0	0.0	0.0	0.019622909155387433	1.3509748127633347E-4
24	0.0	0.0	0.0	0.03053203076845136	1.3509748127633347E-4
25	0.0	0.0	0.0	0.0359359300195047	1.3509748127633347E-4
26	0.0	0.0	0.0	0.045460302449486206	1.3509748127633347E-4
27	0.0	0.0	0.0	0.11040841657308352	1.3509748127633347E-4
28	0.0	0.0	0.0	0.1831584102403891	1.3509748127633347E-4
29	0.0	0.0	0.0	0.2717485835873447	1.3509748127633347E-4
30	0.0	0.0	0.0	0.4202207155100352	1.3509748127633347E-4
31	0.0	0.0	0.0	0.6570803745577668	1.3509748127633347E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7555	0.0	25.735935	1
ACCGTTA	125	8.594725E-9	17.76	8
ACGTTTA	170	5.456968E-12	17.411766	26
GTATTGG	1115	0.0	16.923767	1
TCTAACG	100	5.8840888E-6	16.65	2
CCCGTAT	90	4.4497647E-5	16.444445	1
ATTGGAC	1070	0.0	16.425234	3
CGAACGA	230	0.0	16.086956	16
TTACGGC	140	3.478999E-8	15.857143	18
CGCGATA	130	2.5927875E-7	15.653846	14
GTATCAA	12475	0.0	15.600801	2
CGAGCCG	450	0.0	14.8	15
GACCGTG	245	0.0	14.34694	7
TTGGACC	1695	0.0	14.2979355	4
TGGACCC	1640	0.0	13.875001	5
AAGACCG	255	1.8189894E-12	13.784313	5
GGACCCT	1600	0.0	13.759374	6
CGCGTAT	110	2.45841E-4	13.454545	24
TAGTACG	110	2.45841E-4	13.454545	4
ACCGTCG	400	0.0	13.412499	8
>>END_MODULE