FastQCFastQC Report
Wed 25 May 2016
SRR2088411_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088411_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3284409
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT312550.9516171706995079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT304640.9275336902316368No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT281010.8555877176076426No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG142610.4342029266148035No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106160.3232240564436403No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA79570.2422658079429206No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT72180.21976556512906886No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA70800.21556389597032527No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT69190.21066194861845772No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA57700.17567848584022272No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT56660.17251201053218404No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG52330.15932851237467685No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT49590.1509860678131134No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA44890.13667603517101554No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA43010.1309520221141764No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT39340.11977801790215531No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA38100.11600260503487841No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT35520.10814731052070554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75050.027.6822131
GTATTGG10150.019.137931
CAATGCG1900.017.52631419
ATTGGAC10600.017.4528313
GGACCCT15750.017.3841276
TGGACCC17100.016.0116965
GACCCTC16400.015.9054897
CATACGT700.00259386915.8571422
GTATCAA130800.015.8551222
TATACCG1659.767973E-1015.696975
CGATGCG2850.015.57894833
GTACTAG3650.015.205481
TTGGACC18550.015.159034
CTAGACA3350.014.9104484
CGTCGTA3250.014.80000110
CGCATTG1508.115421E-814.814
GTCGTAG3600.014.38888811
GCGCTAG1304.44973E-614.23076911
TACCGTC3950.014.0506327
CCGTCGT3500.013.7428589
Produced by FastQC (version 0.11.5)