##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088411_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3284409
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.503654995464938	31.0	31.0	34.0	30.0	34.0
2	31.698031822467907	31.0	31.0	34.0	30.0	34.0
3	31.78566311321154	31.0	31.0	34.0	30.0	34.0
4	35.554501890598885	37.0	35.0	37.0	33.0	37.0
5	35.38666469370897	37.0	35.0	37.0	33.0	37.0
6	35.421150958970095	37.0	35.0	37.0	33.0	37.0
7	35.30852887079532	37.0	35.0	37.0	32.0	37.0
8	35.33492600951952	37.0	35.0	37.0	32.0	37.0
9	36.87771011466599	39.0	37.0	39.0	33.0	39.0
10	36.739058990521585	39.0	35.0	39.0	32.0	39.0
11	36.84434064088851	39.0	37.0	39.0	32.0	39.0
12	36.710313179631406	39.0	35.0	39.0	32.0	39.0
13	36.745227832465446	39.0	35.0	39.0	32.0	39.0
14	37.794068278341705	40.0	37.0	41.0	32.0	41.0
15	37.78836496916188	40.0	37.0	41.0	32.0	41.0
16	37.74826643088604	40.0	37.0	41.0	32.0	41.0
17	37.79266589514278	40.0	37.0	41.0	32.0	41.0
18	37.76394565963009	40.0	37.0	41.0	32.0	41.0
19	37.82045719640885	40.0	37.0	41.0	32.0	41.0
20	37.77735537809085	40.0	37.0	41.0	32.0	41.0
21	37.73312489400681	40.0	37.0	41.0	32.0	41.0
22	37.624360120801036	39.0	37.0	41.0	32.0	41.0
23	37.51803992742682	39.0	36.0	41.0	32.0	41.0
24	37.447702767834336	39.0	36.0	41.0	32.0	41.0
25	37.39136508272873	39.0	36.0	41.0	32.0	41.0
26	37.185903156397394	39.0	36.0	41.0	31.0	41.0
27	37.02722346699208	39.0	36.0	41.0	31.0	41.0
28	36.92169489244488	39.0	35.0	40.0	31.0	41.0
29	36.782966433230456	39.0	35.0	40.0	30.0	41.0
30	36.6092916564289	38.0	35.0	40.0	30.0	41.0
31	36.39922068171169	38.0	35.0	40.0	30.0	41.0
32	36.26027026475692	38.0	35.0	40.0	30.0	41.0
33	36.35245397269341	38.0	35.0	40.0	30.0	41.0
34	36.34137922530355	38.0	35.0	40.0	30.0	41.0
35	36.273187657201035	38.0	35.0	40.0	30.0	41.0
36	36.20226409073901	38.0	35.0	40.0	30.0	41.0
37	36.091014852291536	38.0	35.0	40.0	29.0	41.0
38	35.98220593111272	38.0	35.0	40.0	29.0	41.0
39	35.94269745333179	38.0	35.0	40.0	28.0	41.0
40	35.80661391440591	38.0	35.0	40.0	27.0	41.0
41	35.6910540069766	38.0	35.0	40.0	27.0	41.0
42	35.53989560983422	38.0	35.0	40.0	26.0	41.0
43	35.21567563601245	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	3.0
14	4.0
15	12.0
16	37.0
17	69.0
18	197.0
19	410.0
20	867.0
21	1692.0
22	3230.0
23	5518.0
24	9234.0
25	14694.0
26	22499.0
27	33568.0
28	47705.0
29	64989.0
30	84873.0
31	106025.0
32	132147.0
33	164162.0
34	203865.0
35	253526.0
36	325902.0
37	449138.0
38	615931.0
39	744108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.54257280381341	21.86646060219662	13.250663970291154	23.340302623698815
2	18.149505740606607	22.723996919993823	35.853725891020275	23.2727714483793
3	19.650963080420254	25.83813404481598	31.54293512166116	22.967967753102613
4	13.164133943123405	16.007781004131946	36.02185355112594	34.806231501618704
5	12.368069871931297	39.29562365710239	34.56192575285234	13.774380718113974
6	31.771591175155105	36.61797297474219	15.899725034245126	15.710710815857587
7	25.71302782327049	33.858176615640744	22.076422272621954	18.352373288466815
8	27.213023712941965	33.551576554564306	20.47138465398189	18.764015078511843
9	25.898510203814446	13.759553088546525	22.515831615368246	37.82610509227079
10	16.306586664450133	27.139159587006368	33.967145991866424	22.587107756677078
11	32.918494621102305	22.1042811659571	24.15049404626525	20.826730166675343
12	20.58379452741726	26.899512210568172	31.365947420068572	21.150745841945994
13	30.333128425844652	20.791533575751377	27.764751588489737	21.110586409914234
14	21.27439061334931	22.095877827639615	27.762589860154446	28.86714169885663
15	23.427685163449496	28.91780530378525	25.597451474527077	22.05705805823818
16	22.622273900723084	27.88361620005304	26.378474788006002	23.115635111217877
17	20.624654237642144	26.990578822552248	27.50942406990116	24.87534286990445
18	21.514007542909546	26.14686538734975	30.671058324343893	21.668068745396813
19	21.14161177855742	25.16939881726058	31.554261360262988	22.134728043919015
20	24.053216271176943	24.44040921821856	30.511760258847175	20.99461425175732
21	23.875680525781046	25.284305334688828	29.597988557454325	21.2420255820758
22	22.720312847760436	24.890566308885404	30.46280167908443	21.926319164269735
23	21.73164182658128	25.969116513808117	31.118596983506013	21.180644676104592
24	20.913260193843094	25.776387776309225	29.988165298536206	23.32218673131148
25	21.96991909351119	26.13200731090434	30.625174879255297	21.272898716329177
26	22.69839109562786	25.743078891818893	29.799211973904587	21.759318038648658
27	21.87589913436481	26.51219747601471	29.486583430991693	22.125319958628783
28	21.507613698537543	26.176033496437256	29.795923711084704	22.520429093940493
29	20.817839678310467	26.20200468333877	30.487220075209876	22.492935563140886
30	20.553499883845163	26.532657778005113	31.461855085648587	21.451987252501134
31	21.64161040844791	26.038322267415538	30.622312872726877	21.697754451409676
32	22.117769132894228	26.066059373238836	29.35785403096874	22.458317462898194
33	22.57252370213332	25.995544403879055	29.295925081194213	22.13600681279341
34	21.849105881758334	27.52805755921385	29.328016090566067	21.294820468461754
35	21.01650555701193	28.20927600673363	29.549852043396545	21.224366392857892
36	21.335284369273133	26.152741634796396	30.689204663609193	21.82276933232128
37	21.583822234076205	25.99791926036008	30.189145139962775	22.229113365600934
38	21.51306368969273	25.701275328377193	30.760663486185795	22.024997495744287
39	22.248386239350822	25.258699510322863	30.665212523775203	21.82770172655111
40	21.06187140517518	25.856493512227008	30.353071130909704	22.728563951688113
41	21.44038699199765	25.475542175167586	30.41268611795912	22.671384714875646
42	20.0294177734868	26.668085491179696	30.661832920321437	22.640663815012076
43	20.264559011986634	26.23044206735519	30.471052783012105	23.033946137646073
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	294.0
1	426.5
2	559.0
3	1478.0
4	2397.0
5	2397.0
6	3969.5
7	5542.0
8	5736.0
9	5930.0
10	8707.0
11	11484.0
12	11484.0
13	20262.0
14	29040.0
15	46637.5
16	64235.0
17	63389.0
18	62543.0
19	62543.0
20	67743.5
21	72944.0
22	61313.0
23	49682.0
24	55851.0
25	62020.0
26	62020.0
27	68566.5
28	75113.0
29	81874.0
30	88635.0
31	96256.0
32	103877.0
33	103877.0
34	114569.0
35	125261.0
36	137430.5
37	149600.0
38	158217.0
39	166834.0
40	166834.0
41	175941.5
42	185049.0
43	201282.0
44	217515.0
45	252810.0
46	288105.0
47	288105.0
48	292531.0
49	296957.0
50	276366.0
51	255775.0
52	244113.5
53	232452.0
54	232452.0
55	209617.5
56	186783.0
57	164445.0
58	142107.0
59	128458.0
60	114809.0
61	114809.0
62	101510.5
63	88212.0
64	77388.0
65	66564.0
66	56814.0
67	47064.0
68	47064.0
69	40063.5
70	33063.0
71	28358.5
72	23654.0
73	18306.0
74	12958.0
75	12958.0
76	9831.5
77	6705.0
78	5404.0
79	4103.0
80	3345.0
81	2587.0
82	2587.0
83	2006.0
84	1425.0
85	1215.0
86	1005.0
87	913.5
88	822.0
89	822.0
90	682.5
91	543.0
92	323.0
93	103.0
94	76.0
95	49.0
96	49.0
97	27.0
98	5.0
99	5.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3284409.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.648431300448465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.15003068812932	31.1973405842838
2	13.934668107531802	11.885834709572407
3	5.159042855176395	6.600752543551799
4	2.51115882881603	4.283879391811004
5	1.3440698289163677	2.8661234880772612
6	0.8174015164690691	2.0916535452008094
7	0.5369682865815177	1.6030598556553965
8	0.3885522442182538	1.3256914955341819
9	0.2961677158444408	1.1367979634342138
>10	1.5871549510302587	12.705230535878286
>50	0.15663362597797922	4.652099171538218
>100	0.10424281395820557	8.672889575968412
>500	0.008345122410339224	2.4127106422548104
>1k	0.004707504949176398	3.682414256905476
>5k	4.992808279429512E-4	1.3945142024643156
>10k+	3.5662916281639374E-4	3.489008037869459
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31255	0.9516171706995079	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	30464	0.9275336902316368	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28101	0.8555877176076426	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14261	0.4342029266148035	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10616	0.3232240564436403	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7957	0.2422658079429206	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7218	0.21976556512906886	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7080	0.21556389597032527	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6919	0.21066194861845772	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5770	0.17567848584022272	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5666	0.17251201053218404	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5233	0.15932851237467685	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4959	0.1509860678131134	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4489	0.13667603517101554	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4301	0.1309520221141764	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3934	0.11977801790215531	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3810	0.11600260503487841	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3552	0.10814731052070554	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.0446877961910346E-5	0.0
3	0.0	0.0	0.0	3.0446877961910346E-5	0.0
4	0.0	0.0	0.0	9.134063388573104E-5	0.0
5	0.0	0.0	0.0	9.134063388573104E-5	0.0
6	0.0	0.0	0.0	9.134063388573104E-5	0.0
7	0.0	0.0	0.0	9.134063388573104E-5	0.0
8	0.0	0.0	0.0	9.134063388573104E-5	0.0
9	0.0	0.0	0.0	9.134063388573104E-5	0.0
10	0.0	3.0446877961910346E-5	0.0	1.2178751184764138E-4	0.0
11	0.0	3.0446877961910346E-5	0.0	2.7402190165719313E-4	0.0
12	0.0	3.0446877961910346E-5	0.0	6.089375592382069E-4	0.0
13	0.0	3.0446877961910346E-5	0.0	6.698313151620276E-4	0.0
14	3.0446877961910346E-5	3.0446877961910346E-5	0.0	8.220657049715793E-4	0.0
15	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.0011874282405145034	0.0
16	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.0017354720438288898	0.0
17	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.002283515847143276	0.0
18	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.002466197114914738	0.0
19	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.0028620065284195727	0.0
20	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.0035622847215435105	0.0
21	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.0054804380331438625	0.0
22	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.009194957144496925	0.0
23	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.015040757713183712	0.0
24	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.024083480467871083	0.0
25	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.029503024745091127	0.0
26	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.03775412867276883	0.0
27	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.1012358692233519	0.0
28	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.16901061956656432	0.0
29	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.24701552090497864	0.0
30	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.3845745155368896	0.0
31	3.0446877961910346E-5	3.0446877961910346E-5	0.0	0.6095160499194833	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7505	0.0	27.682213	1
GTATTGG	1015	0.0	19.13793	1
CAATGCG	190	0.0	17.526314	19
ATTGGAC	1060	0.0	17.452831	3
GGACCCT	1575	0.0	17.384127	6
TGGACCC	1710	0.0	16.011696	5
GACCCTC	1640	0.0	15.905489	7
CATACGT	70	0.002593869	15.857142	2
GTATCAA	13080	0.0	15.855122	2
TATACCG	165	9.767973E-10	15.69697	5
CGATGCG	285	0.0	15.578948	33
GTACTAG	365	0.0	15.20548	1
TTGGACC	1855	0.0	15.15903	4
CTAGACA	335	0.0	14.910448	4
CGTCGTA	325	0.0	14.800001	10
CGCATTG	150	8.115421E-8	14.8	14
GTCGTAG	360	0.0	14.388888	11
GCGCTAG	130	4.44973E-6	14.230769	11
TACCGTC	395	0.0	14.050632	7
CCGTCGT	350	0.0	13.742858	9
>>END_MODULE