FastQCFastQC Report
Wed 25 May 2016
SRR2088410_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088410_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3308589
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT376801.1388540553087736No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT371971.1242556872431118No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT342881.0363330108393638No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG169770.5131190365439768No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123980.37472167138317874No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA94190.28468328946266824No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT90310.2729562360268985No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT85300.2578138293997834No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA82370.24895809059390572No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA71740.21682959110363964No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT69930.21135898112458212No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT66810.20192897939272603No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG61160.18485221343599945No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA54650.16517615213010742No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA53310.16112608728373334No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT47870.14468403298203555No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT47730.14426089187868302No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA47340.143082141662201No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA40800.12331540726273345No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC37170.11234396294009319No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33420.10100982624315077No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG33140.10016354403644574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91250.028.3227391
ATTGGAC8500.019.152943
CGCGATA801.6181666E-518.514
GTATTGG9000.017.8833331
GGACCCT14450.016.8996546
GTATCAA154100.016.7952632
TTGGACC15600.015.6538474
ACGTTTA2400.015.41666726
ATCACGT2550.015.23529523
TAAACGT3200.015.0312514
ATACGAC1001.0943499E-414.819
AATGCGT2151.2732926E-1114.62790735
TGGACCC17600.014.5056825
GACCCTC16450.014.3951367
TCACGTT2700.014.38888824
CGTCTGT9150.013.74863434
CAATGCG1753.5724952E-813.74285819
TGTTACG950.001245938513.63157916
TAGACAG5450.013.5779825
ATAGTAC3000.013.5666663
Produced by FastQC (version 0.11.5)