##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088410_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3308589
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.494386277654915	31.0	31.0	34.0	30.0	34.0
2	31.68197984095335	31.0	31.0	34.0	30.0	34.0
3	31.767511165635863	31.0	31.0	34.0	30.0	34.0
4	35.541945524209865	37.0	35.0	37.0	33.0	37.0
5	35.37537330868234	37.0	35.0	37.0	33.0	37.0
6	35.41452836843742	37.0	35.0	37.0	33.0	37.0
7	35.297606623246345	37.0	35.0	37.0	32.0	37.0
8	35.331550095826344	37.0	35.0	37.0	32.0	37.0
9	36.85315945860909	39.0	37.0	39.0	32.0	39.0
10	36.724441143943835	39.0	35.0	39.0	32.0	39.0
11	36.836664209425834	39.0	37.0	39.0	32.0	39.0
12	36.69840376063633	39.0	35.0	39.0	32.0	39.0
13	36.738060242598884	39.0	35.0	39.0	32.0	39.0
14	37.76399002716868	40.0	37.0	41.0	32.0	41.0
15	37.75517720696043	40.0	37.0	41.0	32.0	41.0
16	37.72324516583958	40.0	37.0	41.0	32.0	41.0
17	37.78239394497171	40.0	37.0	41.0	32.0	41.0
18	37.768786936062476	40.0	37.0	41.0	32.0	41.0
19	37.82897422435969	40.0	37.0	41.0	32.0	41.0
20	37.79344911078408	40.0	37.0	41.0	32.0	41.0
21	37.7487082862211	40.0	37.0	41.0	32.0	41.0
22	37.645249681964124	39.0	37.0	41.0	32.0	41.0
23	37.54225623067719	39.0	36.0	41.0	32.0	41.0
24	37.46957358559797	39.0	36.0	41.0	32.0	41.0
25	37.41166279643679	39.0	36.0	41.0	32.0	41.0
26	37.196206902700816	39.0	36.0	41.0	31.0	41.0
27	37.03876576993999	39.0	36.0	41.0	31.0	41.0
28	36.93386606798246	39.0	35.0	40.0	31.0	41.0
29	36.790318471106566	39.0	35.0	40.0	30.0	41.0
30	36.60022807305471	38.0	35.0	40.0	30.0	41.0
31	36.36699390586138	38.0	35.0	40.0	30.0	41.0
32	36.22058255044673	38.0	35.0	40.0	30.0	41.0
33	36.27962252186657	38.0	35.0	40.0	30.0	41.0
34	36.24669065876723	38.0	35.0	40.0	30.0	41.0
35	36.17214437937139	38.0	35.0	40.0	30.0	41.0
36	36.07851776089445	38.0	35.0	40.0	30.0	41.0
37	35.946176753897205	38.0	35.0	40.0	29.0	41.0
38	35.83191747297715	38.0	35.0	40.0	28.0	41.0
39	35.76291222632971	38.0	35.0	40.0	27.0	41.0
40	35.60509963612888	38.0	35.0	40.0	27.0	41.0
41	35.46698335755816	38.0	35.0	40.0	26.0	41.0
42	35.29988251789509	38.0	34.0	40.0	25.0	41.0
43	34.96679551313264	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	5.0
15	12.0
16	37.0
17	73.0
18	196.0
19	400.0
20	890.0
21	1763.0
22	3202.0
23	5656.0
24	9378.0
25	15337.0
26	23579.0
27	34993.0
28	49470.0
29	67874.0
30	87987.0
31	109589.0
32	135692.0
33	167163.0
34	207736.0
35	256593.0
36	328194.0
37	453854.0
38	603497.0
39	745417.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.5161568874224	22.078988958737394	13.463503626470377	22.941350527369824
2	17.748472233934162	23.040728237928615	36.14359474688455	23.067204781252673
3	19.663034604781675	26.172879133672993	31.984571066397187	22.179515195148145
4	12.985565750233711	16.025623007269864	35.93586268950299	35.052948552993435
5	11.8322644486819	39.986532023167584	34.5463579791869	13.634845548963623
6	31.22089204793947	36.625854707248315	16.021180025684664	16.132073219127548
7	24.907203644816565	34.5881280509607	22.1430948358953	18.361573468327435
8	27.478208988786456	33.44730941195779	20.51007242059984	18.564409178655914
9	25.780869125781415	13.656455969599124	23.161746593487432	37.40092831113203
10	16.128506744113576	27.619749687857876	34.02743586465409	22.22430770337446
11	32.05112511708163	22.17334942478501	24.796975387393235	20.978550070740127
12	20.372914254384572	27.26156074386997	31.90483919277976	20.460685808965696
13	30.601534370089485	21.229110052653866	28.181348605100244	19.98800697215641
14	21.045194794518146	22.31416473910782	28.53316020817333	28.107480258200702
15	23.04571525807527	29.067315402426836	26.479928452884295	21.407040886613597
16	22.029390776551576	28.288796220987255	26.932780106565062	22.749032895896107
17	19.918883850487322	27.17765790794807	27.95681180104268	24.946646440521928
18	20.921426021787536	26.57510497677409	31.586969551068446	20.91649945036993
19	20.3605827136583	25.29238294632546	32.82958384979216	21.51745049022408
20	23.862468260639204	24.485150618586957	31.558226180405	20.094154940368842
21	23.307216459947124	25.34905362981017	30.649500436590944	20.69422947365176
22	22.110633868395258	24.91294627407635	31.501404375097664	21.475015482430727
23	21.211640369958314	26.42401337851271	32.20339546555949	20.160950785969487
24	20.17959921888152	25.872025809189353	30.97967139466401	22.968703577265114
25	21.144663178170514	26.56089952544725	31.765928013422034	20.528509282960197
26	22.264385210734847	25.808131502583127	30.683653968504398	21.243829318177628
27	20.987708053191252	26.984766013548374	30.459177613175886	21.568348320084482
28	20.810532828344652	26.581724112605105	30.56387481189111	22.04386824715914
29	20.004721045738833	26.5352692643299	31.417743334091963	22.042266355839303
30	19.690750347051267	26.84960265539177	32.783884610630096	20.675762386926873
31	20.81748443218544	26.342105350649476	31.83731796243051	21.00309225473457
32	21.675795935971497	26.441543509937315	30.285417741520632	21.597242812570556
33	22.27064165419156	26.369216605628566	29.961019637071878	21.399122103108002
34	20.917919995502615	28.380708513508324	30.083246967211704	20.618124523777357
35	20.001275468182964	29.01448321323682	30.27740828492146	20.706833033658757
36	20.673616457045586	26.327627880041916	31.798993468212583	21.199762194699918
37	20.677031810236933	26.406755266368837	31.192813613295577	21.723399310098657
38	20.849492034217608	26.08504713036282	31.63496584193443	21.430494993485137
39	21.60141377487503	25.634371630927866	31.625294045286374	21.13892054891073
40	20.250686924244746	26.07652385956672	31.1929647351182	22.479824481070327
41	20.845925559203636	25.680947376661166	31.100508404035676	22.372618660099516
42	18.91558607007398	26.9810786410763	31.5451390305656	22.55819625828412
43	19.353113970940484	26.713018752102478	31.26837452460853	22.66549275234851
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	355.0
1	484.0
2	613.0
3	1668.0
4	2723.0
5	2723.0
6	4555.0
7	6387.0
8	6820.0
9	7253.0
10	10764.5
11	14276.0
12	14276.0
13	24753.5
14	35231.0
15	57035.5
16	78840.0
17	77707.5
18	76575.0
19	76575.0
20	81467.0
21	86359.0
22	71543.5
23	56728.0
24	63285.5
25	69843.0
26	69843.0
27	76315.5
28	82788.0
29	87538.0
30	92288.0
31	100716.0
32	109144.0
33	109144.0
34	118075.5
35	127007.0
36	137680.0
37	148353.0
38	155597.5
39	162842.0
40	162842.0
41	171604.5
42	180367.0
43	199042.0
44	217717.0
45	260962.0
46	304207.0
47	304207.0
48	307854.5
49	311502.0
50	285144.0
51	258786.0
52	244259.5
53	229733.0
54	229733.0
55	202687.5
56	175642.0
57	149927.0
58	124212.0
59	110709.0
60	97206.0
61	97206.0
62	86096.5
63	74987.0
64	65936.0
65	56885.0
66	48810.5
67	40736.0
68	40736.0
69	34780.5
70	28825.0
71	25011.5
72	21198.0
73	16899.0
74	12600.0
75	12600.0
76	9918.5
77	7237.0
78	5630.0
79	4023.0
80	3220.0
81	2417.0
82	2417.0
83	1803.5
84	1190.0
85	920.5
86	651.0
87	573.0
88	495.0
89	495.0
90	384.5
91	274.0
92	169.0
93	64.0
94	43.0
95	22.0
96	22.0
97	14.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3308589.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.201578376954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.81123207673808	28.869248746786187
2	14.057412426962548	11.30260334919404
3	5.519384978461404	6.656639634125961
4	2.746321822161409	4.41625887927844
5	1.505278531817924	3.0257286438012256
6	0.9247952776509704	2.2306937902273454
7	0.6165675967203476	1.7350893394970253
8	0.42824147392363215	1.3772786542562558
9	0.3195010847232611	1.1560003109121602
>10	1.7845931030893984	13.5038743590678
>50	0.1617407030064895	4.531507079913823
>100	0.11059910463261471	8.287476264266068
>500	0.007543063216863141	2.006134702479568
>1k	0.00565729741245382	4.487360286750172
>5k	7.543063216605093E-4	2.212976784855738
>10k+	3.7715316083025463E-4	4.201129174588075
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37680	1.1388540553087736	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37197	1.1242556872431118	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34288	1.0363330108393638	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	16977	0.5131190365439768	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12398	0.37472167138317874	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9419	0.28468328946266824	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	9031	0.2729562360268985	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8530	0.2578138293997834	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8237	0.24895809059390572	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	7174	0.21682959110363964	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6993	0.21135898112458212	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6681	0.20192897939272603	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6116	0.18485221343599945	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5465	0.16517615213010742	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5331	0.16112608728373334	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4787	0.14468403298203555	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4773	0.14426089187868302	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4734	0.143082141662201	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4080	0.12331540726273345	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3717	0.11234396294009319	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3342	0.10100982624315077	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3314	0.10016354403644574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.0224364525179766E-5	0.0
10	0.0	0.0	0.0	1.2089745810071907E-4	0.0
11	0.0	0.0	0.0	2.4179491620143813E-4	0.0
12	0.0	0.0	0.0	6.044872905035954E-4	0.0
13	0.0	0.0	0.0	7.253847486043144E-4	0.0
14	0.0	0.0	0.0	9.974040293309323E-4	0.0
15	0.0	0.0	0.0	0.0013600964036330895	0.0
16	0.0	0.0	0.0	0.0021157055167625835	0.0
17	0.0	0.0	0.0	0.0027504171717913587	0.0
18	0.0	0.0	0.0	0.0030526608170431567	0.0
19	0.0	0.0	0.0	0.003626923743021572	0.0
20	0.0	0.0	0.0	0.0043825328561510665	0.0
21	0.0	0.0	0.0	0.005954199811460414	0.0
22	0.0	0.0	0.0	0.00906730935755393	0.0
23	0.0	0.0	0.0	0.015323752814266142	0.0
24	0.0	0.0	0.0	0.02466308145254669	0.0
25	0.0	0.0	0.0	0.03004301833802869	0.0
26	0.0	0.0	0.0	0.04025885354753945	0.0
27	0.0	0.0	0.0	0.11282755277249606	0.0
28	0.0	0.0	0.0	0.18702836768181239	0.0
29	0.0	0.0	0.0	0.2672740554961647	0.0
30	0.0	0.0	0.0	0.4170962304474808	0.0
31	3.0224364525179766E-5	0.0	0.0	0.6487659845329837	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9125	0.0	28.322739	1
ATTGGAC	850	0.0	19.15294	3
CGCGATA	80	1.6181666E-5	18.5	14
GTATTGG	900	0.0	17.883333	1
GGACCCT	1445	0.0	16.899654	6
GTATCAA	15410	0.0	16.795263	2
TTGGACC	1560	0.0	15.653847	4
ACGTTTA	240	0.0	15.416667	26
ATCACGT	255	0.0	15.235295	23
TAAACGT	320	0.0	15.031251	4
ATACGAC	100	1.0943499E-4	14.8	19
AATGCGT	215	1.2732926E-11	14.627907	35
TGGACCC	1760	0.0	14.505682	5
GACCCTC	1645	0.0	14.395136	7
TCACGTT	270	0.0	14.388888	24
CGTCTGT	915	0.0	13.748634	34
CAATGCG	175	3.5724952E-8	13.742858	19
TGTTACG	95	0.0012459385	13.631579	16
TAGACAG	545	0.0	13.577982	5
ATAGTAC	300	0.0	13.566666	3
>>END_MODULE