FastQCFastQC Report
Wed 25 May 2016
SRR2088409_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088409_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1294428
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT130941.0115664988705435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT127210.9827506821545886No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT112540.8694187702985412No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG52870.4084429570435744No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43970.3396867187668994No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT29460.22759087411582568No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT29430.22735911151489305No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA29180.22542775650712127No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25710.1986205489992491No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA24290.18765045255510543No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT22880.17675761031127263No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG20500.15837111063728534No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT19850.15334958761707873No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA17250.1332634955362523No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16420.12685139691045003No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA16290.1258470923064087No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA15610.12059380668526948No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT15030.11611306306723898No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA13610.1051429666230953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29350.028.6166951
CTAGCGG551.9029E-523.54545429
TCTACCG400.001931318323.1255
TGCTACG652.6832804E-622.7692318
GTATTGG2800.020.4821431
CGCAATA759.267256E-619.73333236
CGACGAA801.6169275E-518.510
GCTACGT609.237675E-418.519
ATTGGAC2550.018.1372553
CGTATAC650.001580079717.0769233
GGACCCT5650.017.0265486
GTATCAA50500.016.6316832
ATCACGT904.4466535E-516.44444523
TTGGACC6050.016.2066124
TAGACCA700.002592730315.8571434
GACCCTC5750.015.7652187
TCTATAC1659.731593E-1015.696973
TGGACCC5900.015.3644075
ACGTTTA1001.0935328E-414.826
ACGTTGC1001.0935328E-414.86
Produced by FastQC (version 0.11.5)