##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088409_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1294428
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.454046884029086	31.0	31.0	34.0	30.0	34.0
2	31.658857812099242	31.0	31.0	34.0	30.0	34.0
3	31.743768676202926	31.0	31.0	34.0	30.0	34.0
4	35.525320064151884	37.0	35.0	37.0	33.0	37.0
5	35.35189133733201	37.0	35.0	37.0	33.0	37.0
6	35.38910468562176	37.0	35.0	37.0	33.0	37.0
7	35.27288501175809	37.0	35.0	37.0	32.0	37.0
8	35.300120207535684	37.0	35.0	37.0	32.0	37.0
9	36.824924986171496	39.0	37.0	39.0	32.0	39.0
10	36.69136328942204	39.0	35.0	39.0	32.0	39.0
11	36.79730351939235	39.0	37.0	39.0	32.0	39.0
12	36.658821502625095	39.0	35.0	39.0	32.0	39.0
13	36.701656639071466	39.0	35.0	39.0	32.0	39.0
14	37.72973313309045	40.0	37.0	41.0	32.0	41.0
15	37.72084271971867	40.0	37.0	41.0	32.0	41.0
16	37.674480156486105	39.0	36.0	41.0	32.0	41.0
17	37.729521456581594	39.0	37.0	41.0	32.0	41.0
18	37.70752255050107	39.0	37.0	41.0	32.0	41.0
19	37.76225715142132	40.0	37.0	41.0	32.0	41.0
20	37.72605197044563	40.0	37.0	41.0	32.0	41.0
21	37.68076092297138	40.0	37.0	41.0	32.0	41.0
22	37.58028411004706	39.0	36.0	41.0	32.0	41.0
23	37.477525980587565	39.0	36.0	41.0	32.0	41.0
24	37.400906809803246	39.0	36.0	41.0	32.0	41.0
25	37.34492609863198	39.0	36.0	41.0	31.0	41.0
26	37.135798978390454	39.0	36.0	41.0	31.0	41.0
27	36.970777053648405	39.0	36.0	40.0	31.0	41.0
28	36.86701152941686	39.0	35.0	40.0	31.0	41.0
29	36.720574647643595	39.0	35.0	40.0	30.0	41.0
30	36.54536752913256	38.0	35.0	40.0	30.0	41.0
31	36.33637637628358	38.0	35.0	40.0	30.0	41.0
32	36.181897332257954	38.0	35.0	40.0	30.0	41.0
33	36.26648295617833	38.0	35.0	40.0	30.0	41.0
34	36.25064739019861	38.0	35.0	40.0	30.0	41.0
35	36.18492415182614	38.0	35.0	40.0	30.0	41.0
36	36.11670096753161	38.0	35.0	40.0	30.0	41.0
37	35.99545436285371	38.0	35.0	40.0	29.0	41.0
38	35.89241502810508	38.0	35.0	40.0	29.0	41.0
39	35.84027153306325	38.0	35.0	40.0	28.0	41.0
40	35.698806731622	38.0	35.0	40.0	27.0	41.0
41	35.58091218669559	38.0	35.0	40.0	27.0	41.0
42	35.435093338524815	38.0	34.0	40.0	26.0	41.0
43	35.09970890617323	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	3.0
15	6.0
16	12.0
17	24.0
18	80.0
19	153.0
20	355.0
21	714.0
22	1225.0
23	2157.0
24	3671.0
25	6028.0
26	9171.0
27	13717.0
28	19271.0
29	26514.0
30	34383.0
31	43040.0
32	53592.0
33	66128.0
34	82260.0
35	100652.0
36	129391.0
37	178376.0
38	240255.0
39	283248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.02391172008022	22.22332953242668	13.524506577422615	23.228252170070487
2	17.976743395538417	23.03511666929331	35.83250671339	23.155633221778267
3	19.596532213456445	25.842302545989426	31.99104160293195	22.570123637622178
4	13.350066593120669	16.216738204056156	36.078870358181376	34.3543248446418
5	12.267271721563501	39.39817432873826	34.63746148878115	13.697092460917101
6	31.343651404326856	37.02685665019607	15.87048487826283	15.759007067214245
7	25.134190545939983	34.397818959416824	22.146152586316116	18.321837908327076
8	27.09304804902243	33.71241969425878	20.633747106830196	18.560785149888602
9	25.719236604894206	13.86303448318485	22.97292703804306	37.44480187387788
10	16.36097179603655	27.538032242813042	34.003127250028584	22.097868711121823
11	32.48006069089976	22.342455509306042	24.357785832815733	20.819697966978463
12	20.186522541230566	27.296690121041884	31.559345131594807	20.957442206132747
13	30.126820495230326	21.108860438742056	28.002870766083554	20.761448299944067
14	20.991047783267977	22.498818010735246	28.27264243356911	28.237491772427664
15	23.337412355109745	29.142679237470144	25.838903361175745	21.681005046244366
16	22.362078076185004	28.176383699981766	26.741773200208897	22.719765023624333
17	20.290429440648687	27.399978986857516	27.816919905935283	24.49267166655851
18	21.16795990197987	26.474396412932972	31.008986208580158	21.348657476506997
19	20.778675986613393	25.552290278022415	31.96446615802501	21.70456757733918
20	23.69857574156307	24.81034093823681	30.944633459721203	20.546449860478912
21	23.471911917850974	25.692429397386334	29.948672309313455	20.886986375449233
22	22.401477718343546	25.242423680575516	30.825971008043705	21.530127593037232
23	21.623682429613698	26.330085566752263	31.418201707626842	20.628030296007193
24	20.53818366104565	25.923805727317394	30.475932226435155	23.062078385201804
25	21.471800671802526	26.527392794346227	31.132592929077553	20.868213604773693
26	22.243261116106883	26.269827290509784	30.009780381759356	21.477131211623977
27	21.556085004341686	26.98195650897539	29.747270609103015	21.714687877579905
28	21.179239015225257	26.69225325780963	30.139412929880997	21.989094797084118
29	20.379580787807434	26.631840473166523	30.91944859041986	22.06913014860618
30	20.19378443605979	26.9656558727098	31.823863513459226	21.016696177771184
31	21.225977806413336	26.41676477950106	31.044291378122228	21.312966035963377
32	21.86695590639263	26.460567911077327	29.85820764074943	21.814268541780617
33	22.207260658762017	26.44673940922168	29.62783561542241	21.718164316593892
34	21.453645934729472	27.98062155639402	29.655956144335565	20.909776364540942
35	20.53378017162793	28.465777934346292	29.985677071262362	21.014764822763414
36	21.00935702874165	26.413983628289873	31.020265321825548	21.55639402114293
37	21.131186902631896	26.163371002481405	30.697265510325796	22.0081765845609
38	21.05524602372631	26.110992654670635	31.15167471655434	21.682086605048717
39	21.873445259218744	25.63634284796064	31.096669725933	21.393542166887613
40	20.60724891612357	26.016279005089505	30.83779090069127	22.538681178095654
41	20.99931398270124	25.717923283488926	30.827438837849613	22.455323895960223
42	19.5642399577265	26.928805619161512	31.031621689271244	22.475332733840737
43	19.742851668845233	26.523452830130374	30.899517006739657	22.834178494284735
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	127.0
1	184.5
2	242.0
3	627.5
4	1013.0
5	1013.0
6	1604.0
7	2195.0
8	2314.5
9	2434.0
10	3651.0
11	4868.0
12	4868.0
13	8539.0
14	12210.0
15	19499.0
16	26788.0
17	26369.5
18	25951.0
19	25951.0
20	28897.5
21	31844.0
22	27119.5
23	22395.0
24	25212.0
25	28029.0
26	28029.0
27	30371.5
28	32714.0
29	34518.5
30	36323.0
31	39816.5
32	43310.0
33	43310.0
34	47337.0
35	51364.0
36	56122.5
37	60881.0
38	63495.5
39	66110.0
40	66110.0
41	69804.5
42	73499.0
43	79966.0
44	86433.0
45	100381.0
46	114329.0
47	114329.0
48	115441.0
49	116553.0
50	106926.5
51	97300.0
52	92047.5
53	86795.0
54	86795.0
55	77771.5
56	68748.0
57	60575.5
58	52403.0
59	46982.0
60	41561.0
61	41561.0
62	37369.0
63	33177.0
64	29556.0
65	25935.0
66	21978.5
67	18022.0
68	18022.0
69	15168.0
70	12314.0
71	10340.5
72	8367.0
73	6485.0
74	4603.0
75	4603.0
76	3458.0
77	2313.0
78	1855.0
79	1397.0
80	1117.0
81	837.0
82	837.0
83	637.0
84	437.0
85	344.5
86	252.0
87	216.0
88	180.0
89	180.0
90	151.5
91	123.0
92	74.0
93	25.0
94	22.0
95	19.0
96	19.0
97	11.5
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1294428.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.662188577049115
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.59353756842059	41.119763473741884
2	11.702458606929454	12.091492467326018
3	3.68192050068041	5.706482136955634
4	1.5490044912433865	3.2009984853324744
5	0.8215490478760641	2.1221510918334188
6	0.48839402449442426	1.5138902516000936
7	0.34214676019350865	1.2373235312300412
8	0.2566204732270774	1.0606060224471086
9	0.19556264403600437	0.909287477533295
>10	1.1694567819496782	11.487960352747567
>50	0.1161853004962887	4.188668468401724
>100	0.07278713316260012	6.746005218299567
>500	0.00676739171130483	2.260165555208229
>1k	0.003007729649468814	3.0644429546622045
>5k	1.503864824734407E-4	0.4107626169738376
>10k+	4.5115944742032206E-4	2.879999895707052
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13094	1.0115664988705435	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12721	0.9827506821545886	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11254	0.8694187702985412	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5287	0.4084429570435744	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4397	0.3396867187668994	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	2946	0.22759087411582568	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	2943	0.22735911151489305	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2918	0.22542775650712127	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2571	0.1986205489992491	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2429	0.18765045255510543	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2288	0.17675761031127263	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2050	0.15837111063728534	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1985	0.15334958761707873	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1725	0.1332634955362523	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1642	0.12685139691045003	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1629	0.1258470923064087	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1561	0.12059380668526948	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1503	0.11611306306723898	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1361	0.1051429666230953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	7.725420031087091E-5	0.0	0.0	7.725420031087091E-5	0.0
7	7.725420031087091E-5	0.0	0.0	7.725420031087091E-5	0.0
8	7.725420031087091E-5	0.0	0.0	1.5450840062174181E-4	0.0
9	7.725420031087091E-5	0.0	0.0	1.5450840062174181E-4	0.0
10	7.725420031087091E-5	0.0	0.0	1.5450840062174181E-4	0.0
11	7.725420031087091E-5	0.0	0.0	6.180336024869673E-4	0.0
12	7.725420031087091E-5	0.0	0.0	7.72542003108709E-4	0.0
13	7.725420031087091E-5	0.0	0.0	7.72542003108709E-4	0.0
14	7.725420031087091E-5	0.0	0.0	9.270504037304508E-4	0.0
15	7.725420031087091E-5	0.0	0.0	0.0013133214052848054	0.0
16	7.725420031087091E-5	0.0	0.0	0.0020086092080826433	0.0
17	7.725420031087091E-5	0.0	0.0	0.003012913812123965	0.0
18	7.725420031087091E-5	0.0	0.0	0.003244676413056578	0.0
19	7.725420031087091E-5	0.0	0.0	0.003630947414610932	0.0
20	7.725420031087091E-5	0.0	0.0	0.004403489417719641	0.0
21	7.725420031087091E-5	0.0	0.0	0.006643861226734897	0.0
22	7.725420031087091E-5	7.725420031087091E-5	0.0	0.010429317041967572	0.0
23	7.725420031087091E-5	1.5450840062174181E-4	0.0	0.016841415667769855	0.0
24	7.725420031087091E-5	2.317626009326127E-4	0.0	0.027888766312224395	0.0
25	7.725420031087091E-5	2.317626009326127E-4	0.0	0.03383733973616145	0.0
26	7.725420031087091E-5	2.317626009326127E-4	0.0	0.043262352174087707	0.0
27	7.725420031087091E-5	2.317626009326127E-4	0.0	0.11140055684827584	0.0
28	7.725420031087091E-5	2.317626009326127E-4	0.0	0.17884347371966614	0.0
29	7.725420031087091E-5	2.317626009326127E-4	0.0	0.25949685884421536	0.0
30	7.725420031087091E-5	2.317626009326127E-4	0.0	0.40929275324699405	0.0
31	7.725420031087091E-5	2.317626009326127E-4	0.0	0.6497850788147351	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2935	0.0	28.616695	1
CTAGCGG	55	1.9029E-5	23.545454	29
TCTACCG	40	0.0019313183	23.125	5
TGCTACG	65	2.6832804E-6	22.76923	18
GTATTGG	280	0.0	20.482143	1
CGCAATA	75	9.267256E-6	19.733332	36
CGACGAA	80	1.6169275E-5	18.5	10
GCTACGT	60	9.237675E-4	18.5	19
ATTGGAC	255	0.0	18.137255	3
CGTATAC	65	0.0015800797	17.076923	3
GGACCCT	565	0.0	17.026548	6
GTATCAA	5050	0.0	16.631683	2
ATCACGT	90	4.4466535E-5	16.444445	23
TTGGACC	605	0.0	16.206612	4
TAGACCA	70	0.0025927303	15.857143	4
GACCCTC	575	0.0	15.765218	7
TCTATAC	165	9.731593E-10	15.69697	3
TGGACCC	590	0.0	15.364407	5
ACGTTTA	100	1.0935328E-4	14.8	26
ACGTTGC	100	1.0935328E-4	14.8	6
>>END_MODULE