FastQCFastQC Report
Wed 25 May 2016
SRR2088408_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088408_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450178
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT48911.086459133942574No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT46441.0315919480738729No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41810.928743741364527No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16520.3669659556886387No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG15370.34142050477811No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT9440.20969483182207926No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT9300.20658495084166706No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA8570.19036914287237494No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA8410.1868149931804753No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA7940.17637467846052005No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT7470.16593436374056483No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT6960.15460551159763472No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG6790.15082922754999134No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.1410553158972673No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA6250.13883397233983003No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA5650.12550591099520636No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT5240.1163984024097135No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA4970.11040077480463284No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.10395887848806472No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATAGC250.005492835329.61
GCTGCGA358.860982E-426.4285730
GGTATCA12700.024.0354331
TACTCCG551.8993263E-523.5454545
ACGACAT400.001929372423.12527
GCGCGCT502.6985645E-422.223
TGACGGA450.00382224720.55555537
GACGACA450.00382224720.55555526
ATTAGAT801.6131129E-518.53
CTATAGT500.007028396218.51
TACGACG500.007028396218.55
TGAACAC953.596535E-617.5263165
ACTCCGT650.001577916617.0769216
TCTATAC650.001577916617.0769213
ATTGGAC1851.8189894E-1116.03
GGGTAGA700.002589210415.8571421
TTACAGC700.002589210415.8571424
GTATTGG1302.5785448E-715.6538451
AATACCG1201.9290437E-615.4166675
TAGCCTC855.355153E-415.2352945
Produced by FastQC (version 0.11.5)