##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088408_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	450178
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.417121671872014	31.0	31.0	34.0	30.0	34.0
2	31.626230068994932	31.0	31.0	34.0	30.0	34.0
3	31.708937353668993	31.0	31.0	34.0	30.0	34.0
4	35.49899373136848	37.0	35.0	37.0	33.0	37.0
5	35.31168782126181	37.0	35.0	37.0	33.0	37.0
6	35.34112728742853	37.0	35.0	37.0	32.0	37.0
7	35.215601384341305	37.0	35.0	37.0	32.0	37.0
8	35.249898928868134	37.0	35.0	37.0	32.0	37.0
9	36.75837779722688	39.0	37.0	39.0	32.0	39.0
10	36.62806045608626	39.0	35.0	39.0	32.0	39.0
11	36.72970691593103	39.0	35.0	39.0	32.0	39.0
12	36.59529785995762	39.0	35.0	39.0	32.0	39.0
13	36.62976644793837	39.0	35.0	39.0	32.0	39.0
14	37.639738059167705	39.0	36.0	41.0	32.0	41.0
15	37.63032400517129	39.0	36.0	41.0	32.0	41.0
16	37.60060464971633	39.0	36.0	41.0	32.0	41.0
17	37.65578948771375	39.0	36.0	41.0	32.0	41.0
18	37.638822865622046	39.0	36.0	41.0	32.0	41.0
19	37.689927095504444	40.0	37.0	41.0	32.0	41.0
20	37.660943004767006	39.0	37.0	41.0	32.0	41.0
21	37.60836380276246	39.0	37.0	41.0	32.0	41.0
22	37.51292155547361	39.0	36.0	41.0	32.0	41.0
23	37.38692694889577	39.0	36.0	41.0	32.0	41.0
24	37.312751844825826	39.0	36.0	41.0	31.0	41.0
25	37.25156493653621	39.0	36.0	41.0	31.0	41.0
26	37.036636619292814	39.0	36.0	41.0	31.0	41.0
27	36.86113048616325	39.0	35.0	40.0	31.0	41.0
28	36.75690726779185	39.0	35.0	40.0	30.0	41.0
29	36.60395665714451	39.0	35.0	40.0	30.0	41.0
30	36.430358658130785	38.0	35.0	40.0	30.0	41.0
31	36.201198192714884	38.0	35.0	40.0	30.0	41.0
32	36.035388224213534	38.0	35.0	40.0	30.0	41.0
33	36.1030436849424	38.0	35.0	40.0	30.0	41.0
34	36.060253943995484	38.0	35.0	40.0	30.0	41.0
35	35.99013945594854	38.0	35.0	40.0	29.0	41.0
36	35.90817854270977	38.0	35.0	40.0	29.0	41.0
37	35.77510895690149	38.0	35.0	40.0	28.0	41.0
38	35.66079417474865	38.0	35.0	40.0	27.0	41.0
39	35.597732452496565	38.0	35.0	40.0	27.0	41.0
40	35.457983286611075	38.0	34.0	40.0	26.0	41.0
41	35.323469827490456	38.0	34.0	40.0	25.0	41.0
42	35.14612220055178	38.0	34.0	40.0	24.0	41.0
43	34.80618555327004	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	4.0
17	13.0
18	24.0
19	60.0
20	131.0
21	296.0
22	542.0
23	885.0
24	1457.0
25	2335.0
26	3531.0
27	5293.0
28	7486.0
29	10005.0
30	12569.0
31	15719.0
32	19095.0
33	23275.0
34	29096.0
35	35241.0
36	44689.0
37	60760.0
38	81222.0
39	96447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.023772818751695	22.296958092132446	13.816756927259885	22.862512161855978
2	17.869598247804202	23.20770895068173	35.95622176117003	22.966471040344043
3	19.593582982731274	26.02925953733856	31.8682832124182	22.50887426751196
4	13.265197321948207	16.26467752755577	36.05196166849557	34.41816348200045
5	12.151193527893412	39.72828525605428	34.39839352433926	13.722127691713057
6	31.111249328043574	37.016691175490585	16.122733674235526	15.749325822230317
7	24.76398224702229	34.54366939299566	22.46111538102706	18.231232978954992
8	27.05974081363372	33.8037398540133	20.72535752524557	18.41116180710741
9	25.652075401285714	13.975360857260904	22.996459178369445	37.376104563083935
10	16.38862849806077	27.73591779251763	33.78352562764062	22.091928081780985
11	32.10796618226568	22.716792024488093	24.319935669890576	20.85530612335565
12	20.074948131627934	27.662391320766456	31.686355175064087	20.57630537254153
13	30.002132489815143	21.322010404773224	28.219504284971723	20.456352820439918
14	20.909729040512865	22.68569321468397	28.447191999609046	27.957385745194124
15	23.108414893664282	29.182456717120786	26.272496656877948	21.436631732336984
16	21.969754186121936	28.2752600082634	27.08684120503445	22.668144600580213
17	19.952329967257395	27.569094891354084	28.358560391667297	24.12001474972122
18	20.860637347893498	26.58259621749619	31.445117264726395	21.11164916988391
19	20.681152788452568	25.911528328794386	32.26101675337311	21.146302129379933
20	23.384527897853737	25.041650191701947	31.397136243885754	20.176685666558562
21	23.10175086299197	25.847109365628707	30.356881055937873	20.694258715441446
22	22.23809248786036	25.41061535659228	31.048385305368097	21.302906850179262
23	20.96726183865049	26.613917161656058	31.824522744336686	20.59429825535677
24	20.162024799079475	26.4653092776635	30.90088809315426	22.47177783010276
25	21.16118513121476	26.936011977484466	31.277627960495625	20.625174930805148
26	22.084375513685696	26.426213631052608	30.478388548529693	21.011022306732006
27	21.220939272909828	27.214346325231354	30.216047874396352	21.34866652746247
28	20.829982806800864	27.011093389725843	30.437960095784334	21.72096370768896
29	20.07294892242624	26.912465735775626	31.22942480529924	21.785160536498896
30	19.834820893068965	27.203017473088426	32.227252331300065	20.73490930254255
31	20.743128273705068	26.71432189045222	31.536192350581327	21.006357485261383
32	21.25448156062713	26.805841245018634	30.384647850405837	21.55502934394839
33	21.81115025612091	26.726983548729617	30.127638400810348	21.334227794339128
34	20.93993931289401	28.184407056764215	30.042338808204754	20.833314822137023
35	20.138034288659153	28.729746900115067	30.34355299459325	20.78866581663253
36	20.55453620567864	26.56104918498905	31.45955599784974	21.42485861148257
37	20.702477686603967	26.427324302831323	31.1032524912368	21.76694551932791
38	20.622509318536224	26.069243721372438	31.645260319251495	21.662986640839847
39	21.2076112115652	25.836224782197263	31.551741755483388	21.404422250754145
40	20.12426195860304	26.234289547690025	31.29517657459938	22.34627191910755
41	20.38327061740023	26.040810523837237	31.22942480529924	22.3464940534633
42	19.22483995219669	27.049522633269508	31.405133080692526	22.32050433384128
43	19.422095260097116	26.692330589233592	31.1296864795703	22.755887671098986
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	48.0
1	73.0
2	98.0
3	254.5
4	411.0
5	411.0
6	685.5
7	960.0
8	1008.0
9	1056.0
10	1565.5
11	2075.0
12	2075.0
13	3499.5
14	4924.0
15	7836.5
16	10749.0
17	10481.5
18	10214.0
19	10214.0
20	10852.5
21	11491.0
22	9700.5
23	7910.0
24	8885.0
25	9860.0
26	9860.0
27	10729.5
28	11599.0
29	12296.5
30	12994.0
31	13935.5
32	14877.0
33	14877.0
34	16165.0
35	17453.0
36	19222.0
37	20991.0
38	21926.0
39	22861.0
40	22861.0
41	24105.0
42	25349.0
43	27802.0
44	30255.0
45	35052.0
46	39849.0
47	39849.0
48	40327.0
49	40805.0
50	37441.5
51	34078.0
52	31803.5
53	29529.0
54	29529.0
55	26404.0
56	23279.0
57	20498.5
58	17718.0
59	15862.0
60	14006.0
61	14006.0
62	12550.5
63	11095.0
64	9695.0
65	8295.0
66	6973.0
67	5651.0
68	5651.0
69	4732.5
70	3814.0
71	3146.0
72	2478.0
73	2005.5
74	1533.0
75	1533.0
76	1158.5
77	784.0
78	612.0
79	440.0
80	346.0
81	252.0
82	252.0
83	204.5
84	157.0
85	118.5
86	80.0
87	77.0
88	74.0
89	74.0
90	72.5
91	71.0
92	41.5
93	12.0
94	7.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	450178.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.32414969846467
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.32701728036744	53.802579480035675
2	8.058573742101578	10.044875125177107
3	2.197561961966385	4.108835420677341
4	0.9555873567029213	2.382246778764521
5	0.5091692084695475	1.5866768985252422
6	0.34469485028885727	1.2889688069815568
7	0.23486384408501187	1.0246382564257803
8	0.17429643598744687	0.8690301734712405
9	0.14555666790403418	0.8164526004054663
>10	0.936792217835901	11.118846010506282
>50	0.07439622943975491	3.131766430963423
>100	0.03540974379538887	4.086744533254957
>500	0.004292090157016833	1.9699209862201523
>1k	0.0017883708987570138	3.7684184985913407
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4891	1.086459133942574	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4644	1.0315919480738729	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4181	0.928743741364527	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1652	0.3669659556886387	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1537	0.34142050477811	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	944	0.20969483182207926	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	930	0.20658495084166706	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	857	0.19036914287237494	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	841	0.1868149931804753	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	794	0.17637467846052005	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	747	0.16593436374056483	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	696	0.15460551159763472	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	679	0.15082922754999134	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	635	0.1410553158972673	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	625	0.13883397233983003	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	565	0.12550591099520636	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	524	0.1163984024097135	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	497	0.11040077480463284	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	468	0.10395887848806472	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	4.442687114874561E-4	0.0
12	0.0	0.0	0.0	8.885374229749122E-4	0.0
13	0.0	0.0	0.0	8.885374229749122E-4	0.0
14	0.0	0.0	0.0	0.0011106717787186403	0.0
15	0.0	0.0	0.0	0.0017770748459498243	0.0
16	0.0	0.0	0.0	0.0037762840476433766	0.0
17	0.0	0.0	0.0	0.006664030672311841	0.0
18	0.0	0.0	0.0	0.007108299383799297	0.0
19	0.0	0.0	0.0	0.008663239874005394	0.0
20	0.0	0.0	0.0	0.009996046008467761	0.0
21	0.0	0.0	0.0	0.012661658277392499	0.0
22	0.0	0.0	0.0	0.019992092016935522	0.0
23	0.0	0.0	0.0	0.029099600602428374	0.0
24	0.0	0.0	0.0	0.04620394599469543	0.0
25	0.0	0.0	0.0	0.05442291715721337	0.0
26	0.0	0.0	0.0	0.0617533508967564	0.0
27	0.0	0.0	0.0	0.13017073246582464	0.0
28	0.0	0.0	0.0	0.21124977231228537	0.0
29	0.0	0.0	0.0	0.31343157595440024	0.0
30	0.0	0.0	0.0	0.5109090182105744	0.0
31	0.0	0.0	0.0	0.8056813082825016	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAGC	25	0.0054928353	29.6	1
GCTGCGA	35	8.860982E-4	26.42857	30
GGTATCA	1270	0.0	24.035433	1
TACTCCG	55	1.8993263E-5	23.545454	5
ACGACAT	40	0.0019293724	23.125	27
GCGCGCT	50	2.6985645E-4	22.2	23
TGACGGA	45	0.003822247	20.555555	37
GACGACA	45	0.003822247	20.555555	26
ATTAGAT	80	1.6131129E-5	18.5	3
CTATAGT	50	0.0070283962	18.5	1
TACGACG	50	0.0070283962	18.5	5
TGAACAC	95	3.596535E-6	17.526316	5
ACTCCGT	65	0.0015779166	17.076921	6
TCTATAC	65	0.0015779166	17.076921	3
ATTGGAC	185	1.8189894E-11	16.0	3
GGGTAGA	70	0.0025892104	15.857142	1
TTACAGC	70	0.0025892104	15.857142	4
GTATTGG	130	2.5785448E-7	15.653845	1
AATACCG	120	1.9290437E-6	15.416667	5
TAGCCTC	85	5.355153E-4	15.235294	5
>>END_MODULE