FastQCFastQC Report
Wed 25 May 2016
SRR2088407_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088407_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2304333
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT147200.6387965628231683No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT139910.6071605102213959No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122390.5311298323636384No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53150.23065242740524047No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG34740.15075946054671785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42200.023.4976311
TATACCG1800.019.5277795
CGCATAC852.7250382E-517.41176437
TATACTG3650.016.7260275
ACGTTTA1554.0199666E-1016.70967926
CGGGATA1352.2224413E-816.44444524
TACGTTA1059.3511735E-615.85714219
CTAGTAC2350.015.7446793
CGTACGA1201.9374584E-615.41666612
GTATCAA65100.015.2603682
ATCACGT1701.4879333E-915.23529423
CCCGTAT855.367055E-415.2352941
ATACCGA3050.015.1639356
GTACTAG2350.014.9574461
TAGTGCG750.004106069414.87
TACCGAC2251.8189894E-1214.87
ACCGACC2900.014.6724158
CAATGCG1651.6474587E-814.57575919
CGAATTA908.278461E-414.38888915
TAGTACT2850.014.2807024
Produced by FastQC (version 0.11.5)