FastQCFastQC Report
Wed 25 May 2016
SRR2088406_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088406_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3351786
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT226770.6765646732816475No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT213410.6367053266527158No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT196010.5847927045461733No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG82690.24670429436724184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79090.2359637518624399No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT43690.13034841723188773No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT42830.1277826209668517No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA40300.12023440637319925No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39420.11760894042758101No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA35340.10543632558880549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55100.028.1361161
GTATTGG7200.016.701391
CGTATAC1452.9849616E-916.5862063
TATACTG5150.016.5242735
GTATCAA95750.016.133162
ACGTTTA2200.015.97727326
ATTGGAC7600.014.8486843
ACTACGC1001.09435736E-414.7999998
CCCGTAT1152.2122329E-514.4782611
ACGGACC5250.014.44761858
TCTATAC3150.014.0952393
CGAATTA2151.9826984E-1013.76744215
CAAACGT1356.575492E-613.7037034
TTGGACC13050.013.4674344
GAACCGT1805.1682946E-813.3611126
AAGACGG6050.013.148765
ACCCTCG10450.013.1004798
GACGGAC5950.013.0588247
GGACCCT12050.012.8962656
TTATACT7200.012.8472224
Produced by FastQC (version 0.11.5)