##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088406_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3351786
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.417968211574365	31.0	31.0	34.0	30.0	34.0
2	31.628446147814927	31.0	31.0	34.0	30.0	34.0
3	31.724022058687517	31.0	31.0	34.0	30.0	34.0
4	35.501907042991405	37.0	35.0	37.0	33.0	37.0
5	35.30548668679922	37.0	35.0	37.0	33.0	37.0
6	35.32693316339408	37.0	35.0	37.0	32.0	37.0
7	35.21554747230282	37.0	35.0	37.0	32.0	37.0
8	35.228975537221054	37.0	35.0	37.0	32.0	37.0
9	36.770189683947606	39.0	37.0	39.0	32.0	39.0
10	36.61567325598949	39.0	35.0	39.0	32.0	39.0
11	36.71509577282082	39.0	35.0	39.0	32.0	39.0
12	36.587320013867235	39.0	35.0	39.0	32.0	39.0
13	36.623732839745735	39.0	35.0	39.0	32.0	39.0
14	37.680113527534274	39.0	37.0	41.0	32.0	41.0
15	37.67341411414691	39.0	36.0	41.0	32.0	41.0
16	37.63614443165524	39.0	36.0	41.0	32.0	41.0
17	37.648871079478226	39.0	36.0	41.0	32.0	41.0
18	37.61212141825283	39.0	36.0	41.0	32.0	41.0
19	37.654121713021055	40.0	37.0	41.0	32.0	41.0
20	37.60669237236506	39.0	37.0	41.0	32.0	41.0
21	37.56032515202343	39.0	36.0	41.0	32.0	41.0
22	37.44378847575591	39.0	36.0	41.0	32.0	41.0
23	37.35004024719955	39.0	36.0	41.0	31.0	41.0
24	37.28296108403102	39.0	36.0	41.0	31.0	41.0
25	37.22177101998755	39.0	36.0	41.0	31.0	41.0
26	37.02024920445398	39.0	36.0	41.0	31.0	41.0
27	36.85855093374099	39.0	35.0	40.0	30.0	41.0
28	36.762039998973684	39.0	35.0	40.0	30.0	41.0
29	36.629482908515044	39.0	35.0	40.0	30.0	41.0
30	36.477261078123725	38.0	35.0	40.0	30.0	41.0
31	36.30405521116205	38.0	35.0	40.0	30.0	41.0
32	36.15951525544889	38.0	35.0	40.0	30.0	41.0
33	36.28156869203463	38.0	35.0	40.0	30.0	41.0
34	36.287313092184284	38.0	35.0	40.0	30.0	41.0
35	36.2523568032088	38.0	35.0	40.0	30.0	41.0
36	36.197103574034855	38.0	35.0	40.0	30.0	41.0
37	36.09698769551517	38.0	35.0	40.0	29.0	41.0
38	36.01205088869039	38.0	35.0	40.0	29.0	41.0
39	36.00204786343758	38.0	35.0	40.0	29.0	41.0
40	35.90694125460277	38.0	35.0	40.0	28.0	41.0
41	35.81534650481863	38.0	35.0	40.0	27.0	41.0
42	35.714337072832215	38.0	35.0	40.0	27.0	41.0
43	35.40204923584024	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	4.0
15	11.0
16	31.0
17	95.0
18	196.0
19	464.0
20	999.0
21	1931.0
22	3620.0
23	6251.0
24	10503.0
25	16456.0
26	25035.0
27	36713.0
28	51503.0
29	69899.0
30	90498.0
31	113698.0
32	139867.0
33	170857.0
34	210306.0
35	259159.0
36	328746.0
37	440809.0
38	620278.0
39	753855.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.409475425937096	21.429948093344862	12.790882234128311	24.36969424658973
2	18.245735258754586	22.729374727384148	35.219014579093056	23.805875434768208
3	19.434564139834702	25.651697333899005	31.12066223798297	23.79307628828332
4	13.516435715167974	16.321507399338742	36.35709439683799	33.804962488655306
5	12.874091603700236	38.693520409715894	34.77059692951758	13.661791057066292
6	32.08405906582342	37.062777874243764	16.053680038045385	14.799483021887436
7	26.220230050486514	33.165333347654055	22.503017794095445	18.11141880776398
8	26.781811249286203	33.93214841281633	20.68142178528104	18.604618552616426
9	26.13830357904711	13.854852308590107	21.78265557526644	38.224188537096346
10	16.092674174305877	27.01183786793071	34.41556233005329	22.479925627710124
11	33.66351551083512	22.255985316485003	23.649302192920434	20.43119697975945
12	20.35920551013698	26.62088212075592	31.454185917597364	21.565726451509732
13	29.819863201290296	20.887461192331493	27.14949582103392	22.14317978534429
14	21.283846880439263	21.996601214994037	27.38357997795802	29.33597192660868
15	23.662340018127647	29.048543075244066	24.71709112693949	22.572025779688797
16	23.0089570157522	27.89805196393803	25.9384996536175	23.154491366692266
17	21.236528823737554	27.232138328640314	27.30007225998318	24.231260587638946
18	21.946866536228747	26.395688746238573	29.51799428722478	22.139450430307903
19	21.84140037579965	25.878919477556146	30.124387416141722	22.155292730502484
20	23.798565898896886	25.354781003321815	29.273050248434718	21.57360284934659
21	23.717325628784177	25.961054792877587	28.600543113432657	21.721076464905575
22	23.115706074313813	25.56983053214018	29.20174498013895	22.112718413407062
23	22.292891013925114	26.226555036628234	29.53377691773878	21.94677703170787
24	21.81690597192064	26.17867011796099	28.910109416293285	23.09431449382508
25	22.49248609547268	26.400163972282243	29.174654945154614	21.932694987090464
26	22.828008709386577	26.32718795293017	28.638701874164994	22.206101463518255
27	22.49290378323676	26.735835760397592	28.477086544308023	22.294173912057634
28	22.158485058413635	26.439098438862146	28.822126472274785	22.580290030449437
29	21.540963534068105	26.508016919934622	29.400266007436038	22.55075353856123
30	21.47189587879417	26.789001445796362	29.830007046989277	21.909095628420193
31	22.173521817920356	26.475049421412944	29.296977790348187	22.054450970318513
32	22.428460528207946	26.397628010857492	28.512291655851534	22.66161980508302
33	22.548247411976778	26.36886722481686	28.58291072282061	22.49997464038575
34	22.322069487729827	27.098299235094363	28.65499169696395	21.924639580211863
35	21.901875597069743	27.425796276969947	28.918045483810722	21.75428264214959
36	21.939348156475383	26.425732430411724	29.309329414228714	22.325589998884176
37	22.09052129223047	25.994559318524512	29.264875502194947	22.650043887050067
38	21.94442007932487	25.862838498639235	29.668212708090554	22.52452871394534
39	22.3695068837927	25.550229042068917	29.698465236145744	22.38179883799264
40	21.679277853657723	25.645879540042234	29.671763054085194	23.00307955221485
41	21.608569282167775	25.550199207228623	29.892123184475384	22.94910832612822
42	20.926067475668194	26.332707398384024	29.87920469862933	22.86202042731845
43	20.89008665827711	25.80298980901525	29.815029957163137	23.491893575544502
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	282.0
1	412.0
2	542.0
3	1432.0
4	2322.0
5	2322.0
6	3551.0
7	4780.0
8	4902.0
9	5024.0
10	7385.5
11	9747.0
12	9747.0
13	16469.5
14	23192.0
15	35736.5
16	48281.0
17	48877.0
18	49473.0
19	49473.0
20	55569.0
21	61665.0
22	55942.5
23	50220.0
24	57400.0
25	64580.0
26	64580.0
27	72337.0
28	80094.0
29	87129.0
30	94164.0
31	103636.0
32	113108.0
33	113108.0
34	125738.0
35	138368.0
36	151524.5
37	164681.0
38	174372.5
39	184064.0
40	184064.0
41	193301.0
42	202538.0
43	213922.5
44	225307.0
45	247937.0
46	270567.0
47	270567.0
48	273985.5
49	277404.0
50	260336.0
51	243268.0
52	232368.5
53	221469.0
54	221469.0
55	204914.0
56	188359.0
57	171479.0
58	154599.0
59	142467.5
60	130336.0
61	130336.0
62	117077.0
63	103818.0
64	91618.0
65	79418.0
66	67919.5
67	56421.0
68	56421.0
69	47765.5
70	39110.0
71	33496.5
72	27883.0
73	21754.5
74	15626.0
75	15626.0
76	12043.5
77	8461.0
78	6736.5
79	5012.0
80	4094.5
81	3177.0
82	3177.0
83	2424.5
84	1672.0
85	1429.5
86	1187.0
87	1003.0
88	819.0
89	819.0
90	709.0
91	599.0
92	342.5
93	86.0
94	65.5
95	45.0
96	45.0
97	27.5
98	10.0
99	9.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3351786.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.50499099534945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.1761454948933	35.23737123804637
2	13.769196204721961	13.082110834370333
3	5.054085352735516	7.202828375142848
4	2.326488016639961	4.420791691250789
5	1.2586645526059481	2.9896424118855554
6	0.7301179262106706	2.0810547306108673
7	0.49368248208634147	1.641666730625139
8	0.34513157985655424	1.311637807463707
9	0.24589999137104376	1.0513329188254146
>10	1.3896231115893969	12.129525863251157
>50	0.11533779878523272	3.8064409920441125
>100	0.08556535869877649	7.869570728076983
>500	0.007043490864285693	2.3751590109508633
>1k	0.002704197384640683	2.4169243815346424
>5k	1.2577662254142712E-4	0.4833191511163947
>10k+	1.8866493381214066E-4	1.900623134804915
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22677	0.6765646732816475	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21341	0.6367053266527158	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19601	0.5847927045461733	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8269	0.24670429436724184	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7909	0.2359637518624399	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	4369	0.13034841723188773	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	4283	0.1277826209668517	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4030	0.12023440637319925	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3942	0.11760894042758101	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	3534	0.10543632558880549	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.966968058223288E-5	0.0
5	0.0	0.0	0.0	5.966968058223288E-5	0.0
6	0.0	0.0	0.0	8.950452087334931E-5	0.0
7	0.0	0.0	0.0	1.1933936116446576E-4	0.0
8	0.0	0.0	0.0	2.0884388203781505E-4	0.0
9	0.0	0.0	0.0	3.281832432022808E-4	0.0
10	0.0	0.0	0.0	3.878529237845137E-4	0.0
11	0.0	0.0	0.0	4.4752260436674654E-4	0.0
12	0.0	0.0	0.0	5.370271252400959E-4	0.0
13	0.0	0.0	0.0	5.966968058223288E-4	0.0
14	0.0	0.0	0.0	7.45871007277911E-4	0.0
15	0.0	0.0	0.0	9.248800490246096E-4	0.0
16	0.0	0.0	0.0	0.001223228451935774	0.0
17	0.0	0.0	0.0	0.0016409162160114042	0.0
18	0.0	0.0	0.0	0.0018199252577581026	0.0
19	0.0	0.0	0.0	0.001998934299504801	0.0
20	0.0	0.0	0.0	0.002446456903871548	0.0
21	0.0	0.0	0.0	0.0034906763140606233	0.0
22	0.0	0.0	0.0	0.0052210970509453765	0.0
23	0.0	0.0	0.0	0.009069791448499396	0.0
24	0.0	0.0	0.0	0.015961639555747295	0.0
25	0.0	0.0	0.0	0.02070537916203481	0.0
26	0.0	0.0	0.0	0.028372933116851733	0.0
27	0.0	0.0	0.0	0.0885796408243247	0.0
28	0.0	0.0	0.0	0.15269471260993392	0.0
29	0.0	0.0	0.0	0.2231049356969687	0.0
30	0.0	0.0	0.0	0.3390729599085383	0.0
31	0.0	0.0	0.0	0.5407863151167766	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5510	0.0	28.136116	1
GTATTGG	720	0.0	16.70139	1
CGTATAC	145	2.9849616E-9	16.586206	3
TATACTG	515	0.0	16.524273	5
GTATCAA	9575	0.0	16.13316	2
ACGTTTA	220	0.0	15.977273	26
ATTGGAC	760	0.0	14.848684	3
ACTACGC	100	1.09435736E-4	14.799999	8
CCCGTAT	115	2.2122329E-5	14.478261	1
ACGGACC	525	0.0	14.4476185	8
TCTATAC	315	0.0	14.095239	3
CGAATTA	215	1.9826984E-10	13.767442	15
CAAACGT	135	6.575492E-6	13.703703	4
TTGGACC	1305	0.0	13.467434	4
GAACCGT	180	5.1682946E-8	13.361112	6
AAGACGG	605	0.0	13.14876	5
ACCCTCG	1045	0.0	13.100479	8
GACGGAC	595	0.0	13.058824	7
GGACCCT	1205	0.0	12.896265	6
TTATACT	720	0.0	12.847222	4
>>END_MODULE