FastQCFastQC Report
Wed 25 May 2016
SRR2088405_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088405_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2758010
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT334161.2115982175554114No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT320311.1613808506858205No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT286811.039916461506666No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG141430.512797270495756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110650.4011950645574164No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT76830.2785704185264013No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA76170.2761773887694388No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT75990.27552474429026724No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA71960.26091275956214804No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA61850.22425589464867787No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT59370.21526390404675833No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG55710.20199346630360293No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT54440.19738869692278127No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA45480.16490150507068502No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42500.15409661313773337No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA42230.153117646418976No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA40780.14786023255898276No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT39900.14466952621636614No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA33990.12324103248356605No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC30190.10946298236772165No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29580.10725124274386243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75300.028.6467441
GTATTGG8000.018.7312491
ATTGGAC8050.017.9254673
TTGGACC13750.017.2218194
GTATCAA128000.016.9101542
CGTCTGT6250.016.87200234
TATACCG2300.016.0869565
CCGTGCA1504.6784407E-916.0333339
GGACCCT13050.016.0191576
TGCGACG1059.352507E-615.85714222
CGCACTA1302.5926056E-715.65384629
ACGAACG2201.8189894E-1215.13636415
TGGACCC14750.014.6745765
GCGCACT1406.003138E-714.53571528
TGCGACA1152.2119524E-514.4782619
ACGTTTA2059.276846E-1114.43902526
GTCCCGT908.279193E-414.3888886
CGAACGA2201.8189894E-1114.29545416
TATTGGA10300.014.1893212
ATCACGT2101.364242E-1014.09523823
Produced by FastQC (version 0.11.5)