##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088405_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2758010
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.376262957712264	31.0	31.0	34.0	30.0	34.0
2	31.57292286829997	31.0	31.0	34.0	30.0	34.0
3	31.647456318142428	31.0	31.0	34.0	30.0	34.0
4	35.43942516524596	37.0	35.0	37.0	33.0	37.0
5	35.26716690657394	37.0	35.0	37.0	33.0	37.0
6	35.30306416582971	37.0	35.0	37.0	32.0	37.0
7	35.18586988444567	37.0	35.0	37.0	32.0	37.0
8	35.220808118897324	37.0	35.0	37.0	32.0	37.0
9	36.714741425883155	39.0	35.0	39.0	32.0	39.0
10	36.58690468852542	39.0	35.0	39.0	32.0	39.0
11	36.69202649736585	39.0	35.0	39.0	32.0	39.0
12	36.565234716335326	39.0	35.0	39.0	32.0	39.0
13	36.60999488761825	39.0	35.0	39.0	32.0	39.0
14	37.61309313599298	39.0	36.0	41.0	32.0	41.0
15	37.603521742125665	39.0	36.0	41.0	32.0	41.0
16	37.55340988611354	39.0	36.0	41.0	32.0	41.0
17	37.62069753191613	39.0	36.0	41.0	32.0	41.0
18	37.600471716926336	39.0	36.0	41.0	32.0	41.0
19	37.66680541404853	39.0	37.0	41.0	32.0	41.0
20	37.62857495077973	39.0	37.0	41.0	32.0	41.0
21	37.58179919579697	39.0	36.0	41.0	32.0	41.0
22	37.4703496361507	39.0	36.0	41.0	32.0	41.0
23	37.36890112798721	39.0	36.0	41.0	32.0	41.0
24	37.30485277428291	39.0	36.0	41.0	31.0	41.0
25	37.241863517536196	39.0	36.0	41.0	31.0	41.0
26	37.012435777970346	39.0	36.0	41.0	31.0	41.0
27	36.84287076551571	39.0	35.0	40.0	31.0	41.0
28	36.72422942628924	39.0	35.0	40.0	30.0	41.0
29	36.57521401300213	38.0	35.0	40.0	30.0	41.0
30	36.39530748619476	38.0	35.0	40.0	30.0	41.0
31	36.158823572068265	38.0	35.0	40.0	30.0	41.0
32	36.02388751309821	38.0	35.0	40.0	30.0	41.0
33	36.07282932259129	38.0	35.0	40.0	30.0	41.0
34	36.044145960311965	38.0	35.0	40.0	30.0	41.0
35	35.95983118262805	38.0	35.0	40.0	29.0	41.0
36	35.877578036337795	38.0	35.0	40.0	29.0	41.0
37	35.74410716422348	38.0	35.0	40.0	28.0	41.0
38	35.626413247232605	38.0	35.0	40.0	27.0	41.0
39	35.55086384748424	38.0	34.0	40.0	27.0	41.0
40	35.3890196192182	38.0	34.0	40.0	26.0	41.0
41	35.25207812879576	38.0	34.0	40.0	25.0	41.0
42	35.08583870254277	38.0	34.0	40.0	24.0	41.0
43	34.750286982280706	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	5.0
15	4.0
16	28.0
17	66.0
18	155.0
19	384.0
20	897.0
21	1727.0
22	3188.0
23	5510.0
24	9324.0
25	14702.0
26	22298.0
27	32272.0
28	46014.0
29	61891.0
30	79120.0
31	97265.0
32	118841.0
33	145166.0
34	178650.0
35	216848.0
36	275682.0
37	375939.0
38	494935.0
39	577098.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.05634134756582	22.563152417866505	13.766810127591997	22.61369610697568
2	18.020493036645988	23.2270731433171	35.93271960580273	22.81971421423418
3	19.822299411532228	26.113755932719606	32.25187725932828	21.81206739641988
4	13.063223121018416	16.264516807408242	35.98717916178694	34.6850809097864
5	11.731647093375296	40.109898078687166	34.63421089843764	13.524243929499894
6	31.1130489012005	36.85225941892887	15.836925899470996	16.197765780399635
7	24.69516064118694	34.9476615385731	22.01587376405452	18.341304056185436
8	27.325245376195156	33.56318505009047	20.618163095855348	18.493406477859036
9	25.56089354280804	13.720073531277988	23.47660088252037	37.242432043393606
10	16.309875598710665	27.674809010844776	34.04490919177233	21.970406198672233
11	31.82765834786676	22.260796733876962	24.7469008451746	21.16464407308168
12	20.103226601788972	27.568645508899532	31.816744681853947	20.511383207457552
13	30.456452297127278	21.154092987335073	28.492935123512968	19.896519592024685
14	20.883100496372382	22.69085318762441	28.614725834931708	27.8113204810715
15	23.04705204114561	29.220669975815895	26.583442409563418	21.14883557347508
16	21.820805580835454	28.31091982987734	27.356826117381733	22.511448471905467
17	19.670922150391043	27.283911225847625	28.279266572637518	24.76590005112382
18	20.68766973288712	26.47321800863666	31.88447467558131	20.954637582894915
19	20.11667832966523	25.35059698840831	33.150351158987824	21.382373522938643
20	23.708108382493172	24.49657542938568	31.919644961403332	19.875671226717813
21	23.227979593982617	25.264157852944695	30.995101540603553	20.512761012469134
22	21.949992929684807	24.919561567942104	31.85709986548272	21.27334563689037
23	21.012469135354838	26.327932095967743	32.64273878629882	20.016859982378598
24	19.96798416249397	25.84454008506133	31.414389360444666	22.77308639200003
25	21.014608358925457	26.500339012548903	32.158984195126195	20.326068433399445
26	22.01261054165866	25.81237921544882	31.069212947016144	21.105797295876375
27	20.987958709359283	26.919264252123813	30.73625548855878	21.35652154995812
28	20.70021501009786	26.524378084198393	30.995971733242445	21.779435172461305
29	19.875163614345126	26.54812709163491	31.816454617640982	21.760254676378985
30	19.49427304469527	26.798706313610175	33.153324317170714	20.55369632452384
31	20.613594584501143	26.262015003571413	32.246764877574776	20.877625534352667
32	21.467144789177702	26.38761280778532	30.68404393022505	21.46119847281192
33	22.007425643851906	26.370644051326863	30.348910990170445	21.27301931465078
34	20.894594290811128	28.379737564403317	30.393000750541148	20.3326673942444
35	19.88049354425836	29.08724043785193	30.648075967817373	20.384190050072334
36	20.52871454418222	26.42452347888514	32.03958651346442	21.007175463468226
37	20.69173063186863	26.399868020783103	31.467652401550396	21.44074894579788
38	20.756850047679304	26.1316673978702	31.88179158161138	21.229690972839112
39	21.564135010387925	25.740443290633465	31.851008517010452	20.844413181968157
40	20.151957389567116	26.239933865359443	31.412902781353225	22.19520596372022
41	20.845863503032984	25.803604773006622	31.29713090235351	22.053400821606882
42	19.00660258664762	27.22698612405321	31.586397438732998	22.18001385056617
43	19.472554486749505	26.849286260745973	31.22523123556477	22.45292801693975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	238.0
1	337.5
2	437.0
3	1268.5
4	2100.0
5	2100.0
6	3562.5
7	5025.0
8	5530.5
9	6036.0
10	8829.0
11	11622.0
12	11622.0
13	20886.0
14	30150.0
15	48773.0
16	67396.0
17	65836.5
18	64277.0
19	64277.0
20	69934.5
21	75592.0
22	62628.5
23	49665.0
24	55414.0
25	61163.0
26	61163.0
27	66195.5
28	71228.0
29	75317.0
30	79406.0
31	85829.5
32	92253.0
33	92253.0
34	100098.5
35	107944.0
36	117356.0
37	126768.0
38	131327.5
39	135887.0
40	135887.0
41	142994.5
42	150102.0
43	165843.0
44	181584.0
45	217584.0
46	253584.0
47	253584.0
48	257200.0
49	260816.0
50	237437.0
51	214058.0
52	201765.5
53	189473.0
54	189473.0
55	166897.5
56	144322.0
57	122974.0
58	101626.0
59	90171.0
60	78716.0
61	78716.0
62	69842.5
63	60969.0
64	53456.5
65	45944.0
66	39311.5
67	32679.0
68	32679.0
69	27491.0
70	22303.0
71	18824.5
72	15346.0
73	11892.0
74	8438.0
75	8438.0
76	6460.0
77	4482.0
78	3530.0
79	2578.0
80	2148.5
81	1719.0
82	1719.0
83	1277.0
84	835.0
85	687.5
86	540.0
87	465.5
88	391.0
89	391.0
90	319.0
91	247.0
92	144.0
93	41.0
94	30.0
95	19.0
96	19.0
97	13.5
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2758010.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.52019783657368
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.74308493010034	31.355705603071215
2	13.733590420880597	11.679099634046324
3	5.135182834908203	6.550469702018223
4	2.4091432495663354	4.097489903528263
5	1.307909116885799	2.7806277201121277
6	0.8070147871824347	2.0588657044822516
7	0.49275545726618136	1.466644166960655
8	0.35272405103504567	1.1998317145382285
9	0.2571546856010379	0.9840841295722232
>10	1.4859967815942061	11.967884090832587
>50	0.1561292465349808	4.628087064371158
>100	0.10567041465628804	8.510952676406157
>500	0.007333662786269499	2.16217008463796
>1k	0.005201784069330083	4.3009167506732
>5k	6.822011894203388E-4	1.9301477074917022
>10k+	4.263757433877118E-4	4.327023347257848
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33416	1.2115982175554114	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32031	1.1613808506858205	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28681	1.039916461506666	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14143	0.512797270495756	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11065	0.4011950645574164	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	7683	0.2785704185264013	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7617	0.2761773887694388	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	7599	0.27552474429026724	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7196	0.26091275956214804	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6185	0.22425589464867787	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	5937	0.21526390404675833	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	5571	0.20199346630360293	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	5444	0.19738869692278127	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4548	0.16490150507068502	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4250	0.15409661313773337	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4223	0.153117646418976	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4078	0.14786023255898276	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3990	0.14466952621636614	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3399	0.12324103248356605	No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC	3019	0.10946298236772165	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2958	0.10725124274386243	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.6258026620643147E-5	0.0
2	0.0	0.0	0.0	3.6258026620643147E-5	0.0
3	0.0	0.0	0.0	3.6258026620643147E-5	0.0
4	0.0	0.0	0.0	1.4503210648257259E-4	0.0
5	0.0	0.0	0.0	1.4503210648257259E-4	0.0
6	0.0	0.0	0.0	1.4503210648257259E-4	0.0
7	0.0	0.0	0.0	1.4503210648257259E-4	0.0
8	0.0	0.0	0.0	1.4503210648257259E-4	0.0
9	0.0	0.0	0.0	1.8129013310321573E-4	0.0
10	0.0	0.0	0.0	2.1754815972385887E-4	0.0
11	0.0	0.0	0.0	5.076123726890041E-4	0.0
12	0.0	0.0	0.0	6.889025057922198E-4	0.0
13	0.0	0.0	0.0	7.251605324128629E-4	0.0
14	0.0	0.0	0.0	0.0010152247453780081	0.0
15	0.0	0.0	0.0	0.0013415469849637964	0.0
16	0.0	0.0	0.0	0.00195793343751473	0.0
17	0.0	0.0	0.0	0.002501803836824377	0.0
18	0.0	0.0	0.0	0.002973158182892738	0.0
19	0.0	0.0	0.0	0.0034445125289610987	0.0
20	0.0	0.0	0.0	0.00449599530095975	0.0
21	0.0	0.0	0.0	0.006743992951439625	0.0
22	0.0	0.0	0.0	0.009934699294056222	0.0
23	0.0	0.0	0.0	0.01533714526053205	0.0
24	0.0	0.0	0.0	0.024727974155278623	0.0
25	0.0	0.0	0.0	0.030819322627546674	0.0
26	0.0	0.0	0.0	0.04035518362877582	0.0
27	0.0	0.0	0.0	0.10725124274386243	0.0
28	0.0	0.0	0.0	0.17668536372239405	0.0
29	0.0	0.0	0.0	0.2553652814891897	0.0
30	0.0	0.0	0.0	0.39521249016501026	0.0
31	0.0	0.0	0.0	0.6024996283552272	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7530	0.0	28.646744	1
GTATTGG	800	0.0	18.731249	1
ATTGGAC	805	0.0	17.925467	3
TTGGACC	1375	0.0	17.221819	4
GTATCAA	12800	0.0	16.910154	2
CGTCTGT	625	0.0	16.872002	34
TATACCG	230	0.0	16.086956	5
CCGTGCA	150	4.6784407E-9	16.033333	9
GGACCCT	1305	0.0	16.019157	6
TGCGACG	105	9.352507E-6	15.857142	22
CGCACTA	130	2.5926056E-7	15.653846	29
ACGAACG	220	1.8189894E-12	15.136364	15
TGGACCC	1475	0.0	14.674576	5
GCGCACT	140	6.003138E-7	14.535715	28
TGCGACA	115	2.2119524E-5	14.47826	19
ACGTTTA	205	9.276846E-11	14.439025	26
GTCCCGT	90	8.279193E-4	14.388888	6
CGAACGA	220	1.8189894E-11	14.295454	16
TATTGGA	1030	0.0	14.189321	2
ATCACGT	210	1.364242E-10	14.095238	23
>>END_MODULE