FastQCFastQC Report
Wed 25 May 2016
SRR2088404_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088404_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3419727
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT308880.9032299946750135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT298290.872262610436447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT271960.7952681602946667No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG134910.39450517541312513No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104280.30493662213387207No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA71420.20884708048332515No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT70830.2071217965644626No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA68990.2017412501056371No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT67470.1972964508570421No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA58860.17211900248177706No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT56110.1640774248938585No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT50610.14799426971802135No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG49650.14518702808732978No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA43100.12603345237792374No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA39400.1152138752596333No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT38450.11243587572926142No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA37280.10901454999185609No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT36910.10793259228002704No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA72900.029.0061741
ACGTTTA1600.020.812526
CTAATCG953.6106048E-617.52631626
GTATCAA126500.016.7011852
GTATTGG10450.016.4641151
ATTGGAC9550.016.0785353
TATACCG2100.015.8571425
CTGTGCG3200.015.6093759
TATACTG5950.015.5462185
CTAACGG1201.938126E-615.41666627
TACCGTC3050.015.1639347
ATATCGA750.0041066114.86
TGCGTCG1406.0043567E-714.53571410
TAGGTCG1956.7484507E-1014.2307721
TACGTTA3900.014.2307719
ACGTTAG800.006301976713.8751
TTACTCG800.006301976713.8754
TGCGACG2151.9826984E-1013.76744222
ATCACGT2304.0017767E-1113.67391323
CGAATTA5500.013.45454515
Produced by FastQC (version 0.11.5)