FastQCFastQC Report
Wed 25 May 2016
SRR2088403_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088403_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2551514
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT251650.986277167203472No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT242990.9523365343086497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT220500.8641927890656292No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG100790.3950203682989786No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87520.34301203128809016No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA58250.22829582749692928No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA56010.21951672614769113No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT53690.21042408546455166No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT53510.20971862196327357No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT43710.17131005356035672No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA42590.16692050288573765No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG39180.1535558887781921No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT35360.13858438558440206No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33930.1329798699909152No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT32470.12725777714721534No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA31910.12506300180990582No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA28140.11028746069980412No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT25600.10033258684843586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67600.025.9437851
GTATTGG8600.018.51
TATACCG1452.9849616E-916.5862055
GTATCAA111400.015.7598752
AACGGTA957.065074E-515.57894818
ATTGGAC9250.015.23
TACGTTA1508.1130565E-814.819
TAGTACT4000.014.84
TTATGCG1252.9619878E-614.7999994
TAGTGCG908.278891E-414.3888897
TATTGGA12350.014.230772
TAACGAA2500.014.05999913
CGTCTGT5800.014.03448134
ATTAGAC2405.456968E-1213.8750013
TGCGTTA2009.858923E-1013.87500137
CTTAACG1753.571222E-813.74285830
TCTAACG1356.574017E-613.7037042
ACGAACG2304.0017767E-1113.67391315
CGAACGA2254.0745363E-1013.15555616
ACGTTAA1703.7362224E-713.05882420
Produced by FastQC (version 0.11.5)