##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088403_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2551514
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.4030740180144	31.0	31.0	34.0	30.0	34.0
2	31.60352637688839	31.0	31.0	34.0	30.0	34.0
3	31.68801856466396	31.0	31.0	34.0	30.0	34.0
4	35.47949021639701	37.0	35.0	37.0	33.0	37.0
5	35.29567033533816	37.0	35.0	37.0	33.0	37.0
6	35.325802641098576	37.0	35.0	37.0	32.0	37.0
7	35.20925419182493	37.0	35.0	37.0	32.0	37.0
8	35.23865359939236	37.0	35.0	37.0	32.0	37.0
9	36.75721238449015	39.0	37.0	39.0	32.0	39.0
10	36.6215658624644	39.0	35.0	39.0	32.0	39.0
11	36.722079126354	39.0	35.0	39.0	32.0	39.0
12	36.59335280935162	39.0	35.0	39.0	32.0	39.0
13	36.63398162816273	39.0	35.0	39.0	32.0	39.0
14	37.66567261633681	39.0	37.0	41.0	32.0	41.0
15	37.64694373615038	39.0	36.0	41.0	32.0	41.0
16	37.6020582289574	39.0	36.0	41.0	32.0	41.0
17	37.64830802417702	39.0	36.0	41.0	32.0	41.0
18	37.62442298964458	39.0	36.0	41.0	32.0	41.0
19	37.67715677828928	40.0	37.0	41.0	32.0	41.0
20	37.643757784593774	39.0	37.0	41.0	32.0	41.0
21	37.594203676719	39.0	36.0	41.0	32.0	41.0
22	37.481880953818006	39.0	36.0	41.0	32.0	41.0
23	37.38640587510004	39.0	36.0	41.0	32.0	41.0
24	37.30956012782999	39.0	36.0	41.0	31.0	41.0
25	37.255517704390414	39.0	36.0	41.0	31.0	41.0
26	37.03984967356636	39.0	36.0	41.0	31.0	41.0
27	36.87613393459726	39.0	35.0	40.0	31.0	41.0
28	36.76593504875929	39.0	35.0	40.0	30.0	41.0
29	36.62331580387174	39.0	35.0	40.0	30.0	41.0
30	36.45498829322512	38.0	35.0	40.0	30.0	41.0
31	36.24749188129087	38.0	35.0	40.0	30.0	41.0
32	36.107109739550715	38.0	35.0	40.0	30.0	41.0
33	36.17817342957946	38.0	35.0	40.0	30.0	41.0
34	36.152477705393736	38.0	35.0	40.0	30.0	41.0
35	36.06598983975788	38.0	35.0	40.0	30.0	41.0
36	35.99320246724102	38.0	35.0	40.0	29.0	41.0
37	35.87150060709053	38.0	35.0	40.0	29.0	41.0
38	35.76271931096596	38.0	35.0	40.0	28.0	41.0
39	35.70800826489684	38.0	35.0	40.0	27.0	41.0
40	35.56952303612679	38.0	34.0	40.0	27.0	41.0
41	35.445382623806886	38.0	34.0	40.0	26.0	41.0
42	35.30122546848656	38.0	34.0	40.0	25.0	41.0
43	34.96423848742354	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	4.0
15	9.0
16	23.0
17	67.0
18	135.0
19	314.0
20	760.0
21	1536.0
22	2858.0
23	4847.0
24	8052.0
25	13023.0
26	19757.0
27	29344.0
28	40812.0
29	55307.0
30	71721.0
31	88223.0
32	108280.0
33	132322.0
34	162879.0
35	199251.0
36	253145.0
37	343035.0
38	462889.0
39	552920.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61497840105914	21.962254567288284	13.212821877520561	23.209945154132015
2	18.1530260073039	22.919646923356094	35.71197336169819	23.215353707641817
3	19.790250024103337	26.067542643309032	31.474841995771925	22.66736533681571
4	13.204513085172175	16.091073770318328	36.0286872813553	34.67572586315419
5	12.29046754201623	39.52273042593535	34.45115331524734	13.735648716801082
6	31.63333612905906	36.72066859127561	15.898639004136367	15.74735627552896
7	25.46950555630892	34.03681108549669	22.221865135758613	18.271818222435776
8	27.264047933893366	33.597581671117624	20.463889282990415	18.6744811119986
9	25.90015183142244	13.691714017638153	22.67238196615813	37.735752184781276
10	16.511804363997218	27.1242485833901	33.784843038290205	22.579104014322475
11	32.61977006592948	22.292920987304008	24.098398049158266	20.988910897608243
12	20.348781155031876	27.125150008975062	31.42236334976018	21.10370548623288
13	30.219430502830868	20.927182841246413	27.918247754078557	20.935138901844162
14	21.236489394140108	22.30420840332446	27.82861469699951	28.630687505535928
15	23.488485659886642	28.917967920223052	25.632467625104155	21.961078794786154
16	22.52035458163271	27.969746589671857	26.63132555808042	22.878573270615014
17	20.504100702563264	27.063892261614086	27.695752404258805	24.73625463156385
18	21.31126852527558	26.22830993676695	30.772592272666344	21.687829265291118
19	20.940939379521335	25.33762307398666	31.665669872867642	22.055767673624366
20	23.884564223437536	24.640742711974145	30.59054349691987	20.88414956766845
21	23.75205466244747	25.370348741962616	29.682533585941524	21.19506300964839
22	22.644986466858498	24.984264244679824	30.52677743488768	21.843971853573997
23	21.625905246845598	26.075929820490895	31.30043574128929	20.99772919137422
24	20.799297985431394	25.77387386469367	30.286096803701646	23.14073134617329
25	21.74748012356585	26.23795127128442	30.672847572068974	21.341721033080752
26	22.588666964006467	25.922256354462487	29.838989713558302	21.65008696797274
27	21.81379369268599	26.711552435142426	29.585336392432097	21.889317479739482
28	21.43390159724775	26.405890777005336	29.872812769202913	22.287394856543997
29	20.63519933655077	26.388175804639914	30.70067418795272	22.2759506708566
30	20.408784745057247	26.635911070838723	31.591165088649326	21.3641390954547
31	21.40517355577904	26.209340807065924	30.827775195432988	21.55771044172205
32	21.94434363283917	26.174851480336773	29.599014545873548	22.28179034095051
33	22.292764217637057	26.17826122059295	29.503816165617746	22.025158396152243
34	21.673602418015342	27.690970929416807	29.502718777949095	21.132707874618756
35	20.795927437591956	28.300530586937793	29.80528423516391	21.098257740306344
36	21.210112897675653	26.310927551249964	30.77333692858436	21.70562262249002
37	21.46102274963022	26.161016557228372	30.37890444653645	21.999056246604955
38	21.283677063892263	25.798133970654284	30.938454580300167	21.979734385153286
39	21.964606112292547	25.4548084000323	30.930576904535894	21.650008583139265
40	20.898885916361813	25.884710019227803	30.576669381394733	22.639734683015654
41	21.27666162129622	25.65077048372065	30.560051796697962	22.512516098285175
42	19.891092112369364	26.823525169761954	30.773493698251315	22.511889019617374
43	20.08219433638224	26.398875334409293	30.487859365067173	23.03107096414129
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	362.5
2	467.0
3	1275.5
4	2084.0
5	2084.0
6	3416.5
7	4749.0
8	5004.0
9	5259.0
10	7646.0
11	10033.0
12	10033.0
13	17291.5
14	24550.0
15	38884.0
16	53218.0
17	51993.5
18	50769.0
19	50769.0
20	55173.5
21	59578.0
22	50004.0
23	40430.0
24	45782.0
25	51134.0
26	51134.0
27	55974.0
28	60814.0
29	65298.5
30	69783.0
31	76026.0
32	82269.0
33	82269.0
34	89658.0
35	97047.0
36	107147.5
37	117248.0
38	123329.5
39	129411.0
40	129411.0
41	136131.0
42	142851.0
43	154009.0
44	165167.0
45	191819.5
46	218472.0
47	218472.0
48	223107.5
49	227743.0
50	212148.5
51	196554.0
52	186525.0
53	176496.0
54	176496.0
55	158439.5
56	140383.0
57	123946.5
58	107510.0
59	96949.0
60	86388.0
61	86388.0
62	77527.0
63	68666.0
64	60829.5
65	52993.0
66	45732.0
67	38471.0
68	38471.0
69	32663.0
70	26855.0
71	22848.0
72	18841.0
73	14705.5
74	10570.0
75	10570.0
76	8189.5
77	5809.0
78	4632.0
79	3455.0
80	2832.0
81	2209.0
82	2209.0
83	1685.5
84	1162.0
85	984.0
86	806.0
87	679.0
88	552.0
89	552.0
90	452.5
91	353.0
92	208.5
93	64.0
94	50.0
95	36.0
96	36.0
97	21.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2551514.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.83767966856866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.50680605387745	34.61056788694375
2	13.495110176353972	12.371690747115089
3	4.579847217556213	6.297887090679816
4	2.0431107545133784	3.746058251711674
5	1.1096364595991666	2.5431580291835614
6	0.6703398810152876	1.8436094841027109
7	0.4398625770861705	1.411359593466688
8	0.3116812428201117	1.1429395973671719
9	0.22423980265336854	0.9250769018671343
>10	1.3674763869692819	11.854073789363417
>50	0.14267050917556082	4.552814925622763
>100	0.09706170367517879	8.602673419179052
>500	0.007105989299361417	2.212123719230031
>1k	0.004280716445398252	3.4714123003005795
>5k	4.280716445398252E-4	1.212549427464868
>10k+	3.4245731563186017E-4	3.202004836401741
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25165	0.986277167203472	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	24299	0.9523365343086497	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22050	0.8641927890656292	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10079	0.3950203682989786	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8752	0.34301203128809016	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5825	0.22829582749692928	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5601	0.21951672614769113	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5369	0.21042408546455166	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	5351	0.20971862196327357	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4371	0.17131005356035672	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	4259	0.16692050288573765	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	3918	0.1535558887781921	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	3536	0.13858438558440206	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3393	0.1329798699909152	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3247	0.12725777714721534	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3191	0.12506300180990582	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2814	0.11028746069980412	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2560	0.10033258684843586	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.9192416737670265E-5	0.0
10	0.0	0.0	0.0	3.9192416737670265E-5	0.0
11	0.0	0.0	0.0	1.959620836883513E-4	0.0
12	0.0	7.838483347534053E-5	0.0	3.5273175063903233E-4	0.0
13	0.0	7.838483347534053E-5	0.0	5.486938343273837E-4	0.0
14	3.9192416737670265E-5	7.838483347534053E-5	0.0	7.446559180157349E-4	0.0
15	1.1757725021301078E-4	7.838483347534053E-5	0.0	0.0014893118360314699	0.0
16	1.1757725021301078E-4	7.838483347534053E-5	0.0	0.002547507087948567	0.0
17	1.1757725021301078E-4	1.1757725021301078E-4	0.0	0.0033705478394396426	0.0
18	1.1757725021301078E-4	1.1757725021301078E-4	0.0	0.0038408568402916858	0.0
19	1.1757725021301078E-4	1.1757725021301078E-4	0.0	0.004311165841143729	0.0
20	1.1757725021301078E-4	1.1757725021301078E-4	0.0	0.005330168676323155	0.0
21	1.1757725021301078E-4	1.1757725021301078E-4	0.0	0.007368174346682009	0.0
22	1.1757725021301078E-4	1.5676966695068106E-4	0.0	0.011757725021301079	0.0
23	1.1757725021301078E-4	1.959620836883513E-4	0.0	0.01904751453450775	0.0
24	1.1757725021301078E-4	1.959620836883513E-4	0.0	0.029276735303039685	0.0
25	1.1757725021301078E-4	1.959620836883513E-4	0.0	0.036723294483197035	0.0
26	1.1757725021301078E-4	1.959620836883513E-4	0.0	0.04585512758307421	0.0
27	1.1757725021301078E-4	1.959620836883513E-4	0.0	0.11354043128903075	0.0
28	1.1757725021301078E-4	1.959620836883513E-4	0.0	0.18647751883783512	0.0
29	1.1757725021301078E-4	1.959620836883513E-4	0.0	0.27344549157872544	0.0
30	1.1757725021301078E-4	1.959620836883513E-4	0.0	0.4235524476840025	0.0
31	1.1757725021301078E-4	1.959620836883513E-4	0.0	0.6406392439939581	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6760	0.0	25.943785	1
GTATTGG	860	0.0	18.5	1
TATACCG	145	2.9849616E-9	16.586205	5
GTATCAA	11140	0.0	15.759875	2
AACGGTA	95	7.065074E-5	15.578948	18
ATTGGAC	925	0.0	15.2	3
TACGTTA	150	8.1130565E-8	14.8	19
TAGTACT	400	0.0	14.8	4
TTATGCG	125	2.9619878E-6	14.799999	4
TAGTGCG	90	8.278891E-4	14.388889	7
TATTGGA	1235	0.0	14.23077	2
TAACGAA	250	0.0	14.059999	13
CGTCTGT	580	0.0	14.034481	34
ATTAGAC	240	5.456968E-12	13.875001	3
TGCGTTA	200	9.858923E-10	13.875001	37
CTTAACG	175	3.571222E-8	13.742858	30
TCTAACG	135	6.574017E-6	13.703704	2
ACGAACG	230	4.0017767E-11	13.673913	15
CGAACGA	225	4.0745363E-10	13.155556	16
ACGTTAA	170	3.7362224E-7	13.058824	20
>>END_MODULE