FastQCFastQC Report
Wed 25 May 2016
SRR2088402_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088402_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2868989
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT275280.9595017617704356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT258430.9007702713394858No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237300.8271206337842354No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG122060.4254460369140488No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87740.30582201604816195No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT63760.22223856557135632No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA61610.21474463652527073No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT59390.20700671909163823No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA57870.2017086855334754No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA50700.17671730355187837No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT47620.16598181449981161No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG43620.15203962092569892No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT42460.14799638478920624No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA36500.12722251636377832No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36130.1259328634581729No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA35640.1242249447453441No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA32470.11317575633785978No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA30220.1053332724524214No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT28800.10038379373361139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61000.029.327051
ATTGGAC7350.019.1292513
GTATTGG7750.018.3806461
GTATCAA104550.017.1286472
TTGGACC12300.016.0934964
CGTATAC1802.0190782E-1015.4166673
CAATGCG1701.4897523E-915.23529419
GGACCCT12350.014.8299596
ACCGTGT2251.8189894E-1214.88
TATACTG5200.014.5865385
ACGTTTA1551.2120472E-714.32258126
CCCGTAT1304.449257E-614.2307691
GCACCGT2750.014.1272746
TGCGACG1753.5717676E-813.74285722
CCGTGCA1356.574701E-613.7037049
TGGACCC13650.013.6886445
GACCCTC13750.013.5890917
AATGCGT2202.8558134E-1013.45454535
ATCACGT1805.167385E-813.36111223
CGTAGAC1409.569934E-613.2142853
Produced by FastQC (version 0.11.5)