##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088402_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2868989
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.445994041803576	31.0	31.0	34.0	30.0	34.0
2	31.648157242847567	31.0	31.0	34.0	30.0	34.0
3	31.732542718009725	31.0	31.0	34.0	30.0	34.0
4	35.51174159259586	37.0	35.0	37.0	33.0	37.0
5	35.326981386125915	37.0	35.0	37.0	33.0	37.0
6	35.35830008410628	37.0	35.0	37.0	32.0	37.0
7	35.24642374020953	37.0	35.0	37.0	32.0	37.0
8	35.27184523886289	37.0	35.0	37.0	32.0	37.0
9	36.801203838704154	39.0	37.0	39.0	32.0	39.0
10	36.65626497696575	39.0	35.0	39.0	32.0	39.0
11	36.76223401344515	39.0	35.0	39.0	32.0	39.0
12	36.62803970318464	39.0	35.0	39.0	32.0	39.0
13	36.67160104134244	39.0	35.0	39.0	32.0	39.0
14	37.712448880075875	40.0	37.0	41.0	32.0	41.0
15	37.70943666915419	40.0	37.0	41.0	32.0	41.0
16	37.66586766278992	39.0	36.0	41.0	32.0	41.0
17	37.707154680620945	39.0	37.0	41.0	32.0	41.0
18	37.6823710373236	39.0	37.0	41.0	32.0	41.0
19	37.74020604470773	40.0	37.0	41.0	32.0	41.0
20	37.695624835089994	40.0	37.0	41.0	32.0	41.0
21	37.65432108662668	39.0	37.0	41.0	32.0	41.0
22	37.55370027560231	39.0	36.0	41.0	32.0	41.0
23	37.45529243925299	39.0	36.0	41.0	32.0	41.0
24	37.39051352235927	39.0	36.0	41.0	31.0	41.0
25	37.326560331879975	39.0	36.0	41.0	31.0	41.0
26	37.11273204602736	39.0	36.0	41.0	31.0	41.0
27	36.95805142508389	39.0	36.0	40.0	31.0	41.0
28	36.86744041193605	39.0	35.0	40.0	31.0	41.0
29	36.727322760735575	39.0	35.0	40.0	30.0	41.0
30	36.56296521178715	38.0	35.0	40.0	30.0	41.0
31	36.3509894949057	38.0	35.0	40.0	30.0	41.0
32	36.21607053913417	38.0	35.0	40.0	30.0	41.0
33	36.3072775810573	38.0	35.0	40.0	30.0	41.0
34	36.29397184861985	38.0	35.0	40.0	30.0	41.0
35	36.23932507235127	38.0	35.0	40.0	30.0	41.0
36	36.164601537335976	38.0	35.0	40.0	30.0	41.0
37	36.054112790254685	38.0	35.0	40.0	29.0	41.0
38	35.9634658759584	38.0	35.0	40.0	29.0	41.0
39	35.92734688073046	38.0	35.0	40.0	28.0	41.0
40	35.81052942343104	38.0	35.0	40.0	27.0	41.0
41	35.69713895731214	38.0	35.0	40.0	27.0	41.0
42	35.56141309708751	38.0	35.0	40.0	26.0	41.0
43	35.24233832893748	38.0	34.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	3.0
14	1.0
15	8.0
16	22.0
17	57.0
18	137.0
19	364.0
20	745.0
21	1518.0
22	2930.0
23	5047.0
24	8440.0
25	13381.0
26	20512.0
27	30817.0
28	42834.0
29	59158.0
30	76842.0
31	96829.0
32	118160.0
33	145720.0
34	180415.0
35	221856.0
36	282632.0
37	384865.0
38	526872.0
39	648823.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.14867641528078	22.023332958055956	13.204268123718844	23.623722502944418
2	17.95161989118815	22.937348313290848	35.60905949796253	23.501972297558478
3	19.588050006465693	26.02672927641061	31.610717224778483	22.774503492345215
4	13.261152273501223	16.382809414745054	36.26483057272091	34.09120773903281
5	12.276763696200996	39.322946166750725	34.8131693777843	13.587120759263977
6	31.442818358662233	36.97738123080988	16.01156365535037	15.56823675517752
7	25.36823947390527	33.833939412106496	22.434592813008344	18.36322830097989
8	27.00651692983138	33.86520478119644	20.64159186389352	18.48668642507866
9	25.800796029542113	13.780429273169052	22.56760133970538	37.851173357583455
10	15.928468181648658	27.617533563216867	34.41212217962495	22.041876075509524
11	32.557671012332214	22.371818086440904	24.295561955796973	20.774948945429905
12	20.153615088799572	27.04116328086305	31.776559617342553	21.028662012994822
13	30.17184799244612	21.413571122092137	27.702232389179603	20.71234849628214
14	21.137759677712253	22.159269345403555	28.02192688783401	28.681044089050182
15	23.218910912520055	29.07215747428798	25.817317528927436	21.89161408426453
16	22.272375390773544	28.144478769350457	26.7242920764074	22.858853763468595
17	20.343159210439634	27.13903747975332	27.848729988159594	24.66907332164745
18	21.17780862875389	26.70250739894785	30.71060920763377	21.40907476466449
19	20.881327882400385	25.581311047201645	31.72769222886529	21.809668841532677
20	23.66011162817285	25.040040237170658	30.71043493021409	20.5894132044424
21	23.149792487876393	25.6854940886842	29.938595093951214	21.22611832948819
22	22.186526333840945	25.426796686916543	30.751668967709534	21.63500801153298
23	21.644453847679443	26.448166932672102	31.26028018929316	20.647099030355292
24	20.799034084829184	25.971587900824993	30.2312068815879	22.998171132757918
25	21.65989482706277	26.486995941776005	30.782655492927997	21.070453738233226
26	22.390849180669566	25.987307724079805	29.932042262971382	21.689800832279246
27	21.500884109350018	26.88626551025466	29.782163682049667	21.830686698345655
28	21.409771874343193	26.566431589664514	29.870940599632835	22.152855936359465
29	20.6514559658472	26.46318964624821	30.691368980501494	22.193985407403098
30	20.570521532149478	26.75329183904156	31.452996159971335	21.22319046883763
31	21.290810107672076	26.417807806164472	30.82768877817238	21.463693307991072
32	21.789626938269894	26.58323193292132	29.78941362270821	21.83772750610058
33	22.47746505824874	26.359634003476483	29.48230195375444	21.68059898452033
34	21.432044528577837	27.877590328858005	29.68990121607298	21.00046392649118
35	20.8398498565174	28.42760986535675	29.68648537864732	21.046054899478527
36	21.396526790447783	26.24715535681733	30.82793276655993	21.528385086174957
37	21.340688305183463	26.421816186817026	30.251701906141847	21.985793601857658
38	21.427339038246572	26.220769755478322	30.662682917222757	21.689208289052345
39	21.94410644307106	25.744574133954508	30.861777441461086	21.44954198151335
40	20.945078562518017	26.089190303622633	30.402277596742266	22.56345353711708
41	21.541107337811333	25.630736123421876	30.389346212202277	22.438810326564514
42	19.74214610094357	26.843950952757222	30.788406647777318	22.62549629852188
43	20.28861037808092	26.438023986846936	30.541560110547657	22.731805524524493
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	306.0
1	423.0
2	540.0
3	1313.5
4	2087.0
5	2087.0
6	3383.0
7	4679.0
8	4853.5
9	5028.0
10	7310.5
11	9593.0
12	9593.0
13	17160.0
14	24727.0
15	40067.5
16	55408.0
17	55243.0
18	55078.0
19	55078.0
20	60244.5
21	65411.0
22	56242.0
23	47073.0
24	53596.5
25	60120.0
26	60120.0
27	68098.0
28	76076.0
29	81101.0
30	86126.0
31	94193.5
32	102261.0
33	102261.0
34	111074.5
35	119888.0
36	128625.5
37	137363.0
38	143893.5
39	150424.0
40	150424.0
41	155831.0
42	161238.0
43	174228.5
44	187219.0
45	216396.5
46	245574.0
47	245574.0
48	247773.0
49	249972.0
50	232157.0
51	214342.0
52	204098.0
53	193854.0
54	193854.0
55	174600.0
56	155346.0
57	136484.5
58	117623.0
59	106115.5
60	94608.0
61	94608.0
62	84069.5
63	73531.0
64	64283.0
65	55035.0
66	47586.5
67	40138.0
68	40138.0
69	34421.0
70	28704.0
71	24835.0
72	20966.0
73	16811.0
74	12656.0
75	12656.0
76	9992.0
77	7328.0
78	5585.5
79	3843.0
80	3102.5
81	2362.0
82	2362.0
83	1746.5
84	1131.0
85	897.5
86	664.0
87	519.5
88	375.0
89	375.0
90	296.5
91	218.0
92	134.5
93	51.0
94	34.0
95	17.0
96	17.0
97	10.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2868989.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.81992408123501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.68751153034741	30.696538013021353
2	14.35264979719491	12.291587493608782
3	5.5789545045938835	7.1667122501812255
4	2.7274925279927813	4.671640919231466
5	1.471873731153969	3.1512760712588532
6	0.90666926267697	2.3294105396770304
7	0.5984054301973556	1.7936572563594675
8	0.4239512093039878	1.4522846877235625
9	0.30777709112774115	1.1861092508429902
>10	1.671820939940648	13.568656013635657
>50	0.16720813776218982	4.98189290601388
>100	0.09550944421626319	7.310079315399665
>500	0.0048032577281478675	1.411907485823939
>1k	0.004559024284325912	3.5465713269677623
>5k	4.884668876063477E-4	1.3284173266044852
>10k+	3.256445917375651E-4	3.1132591436499006
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	27528	0.9595017617704356	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25843	0.9007702713394858	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23730	0.8271206337842354	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	12206	0.4254460369140488	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8774	0.30582201604816195	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6376	0.22223856557135632	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6161	0.21474463652527073	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5939	0.20700671909163823	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5787	0.2017086855334754	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5070	0.17671730355187837	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4762	0.16598181449981161	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4362	0.15203962092569892	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4246	0.14799638478920624	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3650	0.12722251636377832	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3613	0.1259328634581729	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3564	0.1242249447453441	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3247	0.11317575633785978	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3022	0.1053332724524214	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	2880	0.10038379373361139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.485548393528173E-5	0.0	0.0	0.0	0.0
7	3.485548393528173E-5	0.0	0.0	0.0	0.0
8	3.485548393528173E-5	0.0	0.0	0.0	0.0
9	3.485548393528173E-5	0.0	0.0	0.0	0.0
10	3.485548393528173E-5	0.0	0.0	3.485548393528173E-5	0.0
11	3.485548393528173E-5	0.0	0.0	3.485548393528173E-5	0.0
12	3.485548393528173E-5	0.0	0.0	1.0456645180584519E-4	0.0
13	3.485548393528173E-5	0.0	0.0	1.3942193574112692E-4	0.0
14	3.485548393528173E-5	0.0	0.0	2.0913290361169039E-4	0.0
15	3.485548393528173E-5	0.0	0.0	4.8797677509394426E-4	0.0
16	3.485548393528173E-5	0.0	0.0	7.319651626409164E-4	0.0
17	3.485548393528173E-5	0.0	0.0	0.001150230969864297	0.0
18	3.485548393528173E-5	0.0	0.0	0.001429074841346551	0.0
19	3.485548393528173E-5	0.0	0.0	0.0017427741967640867	0.0
20	3.485548393528173E-5	0.0	0.0	0.0020216180682463406	0.0
21	3.485548393528173E-5	0.0	0.0	0.002858149682693102	0.0
22	3.485548393528173E-5	0.0	0.0	0.0055420219457097955	0.0
23	3.485548393528173E-5	0.0	0.0	0.009201847758914377	0.0
24	3.485548393528173E-5	0.0	0.0	0.015127280027912271	0.0
25	3.485548393528173E-5	0.0	0.0	0.019553926487693052	0.0
26	3.485548393528173E-5	0.0	0.0	0.02819808650364292	0.0
27	3.485548393528173E-5	0.0	0.0	0.08665073306311039	0.0
28	3.485548393528173E-5	0.0	0.0	0.1432560389740079	0.0
29	3.485548393528173E-5	0.0	0.0	0.20916775909562568	0.0
30	3.485548393528173E-5	0.0	0.0	0.3152678521946233	0.0
31	3.485548393528173E-5	0.0	0.0	0.47713671959007165	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6100	0.0	29.32705	1
ATTGGAC	735	0.0	19.129251	3
GTATTGG	775	0.0	18.380646	1
GTATCAA	10455	0.0	17.128647	2
TTGGACC	1230	0.0	16.093496	4
CGTATAC	180	2.0190782E-10	15.416667	3
CAATGCG	170	1.4897523E-9	15.235294	19
GGACCCT	1235	0.0	14.829959	6
ACCGTGT	225	1.8189894E-12	14.8	8
TATACTG	520	0.0	14.586538	5
ACGTTTA	155	1.2120472E-7	14.322581	26
CCCGTAT	130	4.449257E-6	14.230769	1
GCACCGT	275	0.0	14.127274	6
TGCGACG	175	3.5717676E-8	13.742857	22
CCGTGCA	135	6.574701E-6	13.703704	9
TGGACCC	1365	0.0	13.688644	5
GACCCTC	1375	0.0	13.589091	7
AATGCGT	220	2.8558134E-10	13.454545	35
ATCACGT	180	5.167385E-8	13.361112	23
CGTAGAC	140	9.569934E-6	13.214285	3
>>END_MODULE