FastQCFastQC Report
Wed 25 May 2016
SRR2088401_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088401_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4233567
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT371330.8771090666570294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT353260.8344263832366419No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT320640.7573755180914817No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG159080.37575878685751285No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125930.2974560223093198No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA85950.20302028998241908No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT81950.19357199260103833No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA81030.19139888420332074No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT80470.19007612256992742No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA65550.1548339733373772No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT65160.15391276434269258No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG60590.14311808458446507No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT60110.14198428889869938No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA51380.12136337986383586No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA47670.11260008404260521No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45920.10846645393825112No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA45710.10797041832572864No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT42930.10140385164566901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82050.029.26631
GTATCAA141600.016.9322032
TTGGACC14050.016.722424
GTATTGG9700.016.5927831
ACGAACG1951.8189894E-1216.12820415
ATTGGAC8700.015.9482753
GGACCCT16250.015.369236
ATTAACG1101.4536203E-515.1363643
GCGCTAT1551.2125201E-714.32258119
TCCGATA2405.456968E-1213.8750018
CCGATAA2304.0017767E-1113.6739139
GACCCTC16550.013.41389757
TATTGGA12850.013.2451362
TGGACCC18000.013.1555565
CTGTGCG4300.012.9069779
CGAACGA2451.1459633E-1012.83673416
ACGGTGT3050.012.73770429
CTAATAC5750.012.5478263
TATCAAC212500.012.3362361
TGTGCGC4200.012.33333310
Produced by FastQC (version 0.11.5)