##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088401_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4233567
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.425682409183555	31.0	31.0	34.0	30.0	34.0
2	31.62645990957507	31.0	31.0	34.0	30.0	34.0
3	31.714088616053555	31.0	31.0	34.0	30.0	34.0
4	35.495386278284954	37.0	35.0	37.0	33.0	37.0
5	35.30774616298738	37.0	35.0	37.0	33.0	37.0
6	35.33850934684629	37.0	35.0	37.0	32.0	37.0
7	35.22376875103193	37.0	35.0	37.0	32.0	37.0
8	35.24939276973767	37.0	35.0	37.0	32.0	37.0
9	36.77692522641073	39.0	37.0	39.0	32.0	39.0
10	36.628650497322944	39.0	35.0	39.0	32.0	39.0
11	36.73457795754738	39.0	35.0	39.0	32.0	39.0
12	36.60488921044594	39.0	35.0	39.0	32.0	39.0
13	36.647894080807035	39.0	35.0	39.0	32.0	39.0
14	37.691228932954175	39.0	37.0	41.0	32.0	41.0
15	37.68578954815171	39.0	36.0	41.0	32.0	41.0
16	37.64490062398918	39.0	36.0	41.0	32.0	41.0
17	37.67840405029612	39.0	37.0	41.0	32.0	41.0
18	37.65522147163373	39.0	36.0	41.0	32.0	41.0
19	37.70370871655037	40.0	37.0	41.0	32.0	41.0
20	37.66369612196996	39.0	37.0	41.0	32.0	41.0
21	37.6174665477126	39.0	37.0	41.0	32.0	41.0
22	37.50831060427294	39.0	36.0	41.0	32.0	41.0
23	37.42174128813835	39.0	36.0	41.0	32.0	41.0
24	37.35501741203104	39.0	36.0	41.0	31.0	41.0
25	37.29332333703471	39.0	36.0	41.0	31.0	41.0
26	37.08707361900733	39.0	36.0	41.0	31.0	41.0
27	36.93318022367427	39.0	36.0	40.0	31.0	41.0
28	36.84246593947846	39.0	35.0	40.0	30.0	41.0
29	36.710363624810945	39.0	35.0	40.0	30.0	41.0
30	36.54672620038847	38.0	35.0	40.0	30.0	41.0
31	36.348192906832466	38.0	35.0	40.0	30.0	41.0
32	36.219144990500915	38.0	35.0	40.0	30.0	41.0
33	36.31656709342264	38.0	35.0	40.0	30.0	41.0
34	36.313599147007714	38.0	35.0	40.0	30.0	41.0
35	36.25968574490495	38.0	35.0	40.0	30.0	41.0
36	36.19045027514623	38.0	35.0	40.0	30.0	41.0
37	36.08711495530837	38.0	35.0	40.0	29.0	41.0
38	36.00207957025364	38.0	35.0	40.0	29.0	41.0
39	35.97287937098905	38.0	35.0	40.0	28.0	41.0
40	35.85665775456016	38.0	35.0	40.0	28.0	41.0
41	35.75619282746677	38.0	35.0	40.0	27.0	41.0
42	35.640935173578214	38.0	35.0	40.0	27.0	41.0
43	35.322647072787554	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	3.0
15	9.0
16	29.0
17	83.0
18	206.0
19	546.0
20	1145.0
21	2243.0
22	4272.0
23	7441.0
24	12470.0
25	20063.0
26	30928.0
27	45292.0
28	64321.0
29	87204.0
30	114664.0
31	141525.0
32	175975.0
33	216122.0
34	266715.0
35	328251.0
36	417520.0
37	560418.0
38	774005.0
39	962116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.1072506942727	21.974377634746304	13.186492619580603	23.73187905140039
2	17.946639323294043	22.90510578904267	35.85685546018287	23.29139942748042
3	19.47988540160106	25.887744306396947	31.647190182652125	22.98518010934987
4	13.241221882162252	16.351034482269917	36.39158185048211	34.01616178508572
5	12.309501656640842	39.282642745467356	34.97341603428031	13.43443956361149
6	31.592177471149036	37.112675906629086	16.033240999847173	15.261905622374702
7	25.455248493764245	33.82261341322813	22.44993406269465	18.27220403031297
8	26.7920408487689	34.08055193173983	20.683409522041345	18.44399769744993
9	25.75475952075401	13.867573136317437	22.47605860495417	37.901608737974385
10	15.866218722887815	27.50841075622519	34.72206770319213	21.90330281769487
11	32.61077479109224	22.367025253173033	24.32126384205092	20.700936113683806
12	20.078127971046637	26.94545757749907	31.928796686104178	21.047617765350118
13	30.137186915903303	21.33279100106364	27.596067335180948	20.933954747852106
14	21.0160132106094	22.264086053202888	28.023791757636058	28.696108978551653
15	23.157044638717185	29.229843297625855	25.701329399062306	21.911782664594657
16	22.3113747815967	28.303461360124928	26.629766341243684	22.75539751703469
17	20.411393040431392	27.320980156922047	27.827219930616426	24.440406872030135
18	21.168650454805604	26.836825778356644	30.617821803694145	21.37670196314361
19	20.89252396383475	25.85663578726875	31.50903245419288	21.741807794703615
20	23.586233547266406	25.255936660504013	30.55307734588823	20.604752446341347
21	23.203908193728832	25.878154284554846	29.671622062435766	21.246315459280556
22	22.328476199857	25.593500705197297	30.348639811298604	21.7293832836471
23	21.66527658591443	26.62374305166305	30.844817148281816	20.866163214140702
24	20.895240349331896	26.267046204772477	29.967141183781905	22.87057226211372
25	21.67696885392389	26.682936634757404	30.544101463375917	21.095993047942788
26	22.251330851738025	26.31218544551202	29.703840756506274	21.732642946243676
27	21.528559722805852	27.03635019830795	29.565753890277396	21.869336188608802
28	21.360852444286344	26.75679869953635	29.721438210379098	22.16091064579821
29	20.761712286589535	26.690944066788123	30.386976277923555	22.160367368698783
30	20.519694149165467	26.942221535645945	31.26595138331341	21.27213293187518
31	21.38383542766655	26.56910827205522	30.61732576808162	21.429730532196608
32	21.850746663510936	26.709911523781248	29.54267642392337	21.896665388784445
33	22.33969605299739	26.600405757131046	29.29248550926441	21.76741268060716
34	21.54211802954813	27.866005191366995	29.44098912335626	21.15088765572861
35	20.734666535335332	28.467200353744254	29.66118169382934	21.136951417091073
36	21.183011866825304	26.46867287089114	30.734272068919662	21.614043193363894
37	21.301635240448537	26.476562199204594	30.28070655312648	21.94109600722039
38	21.318453209787396	26.258967910511394	30.71634392463849	21.706234955062715
39	21.853274083060455	25.791773225745573	30.866146679620282	21.48880601157369
40	20.83290520735824	26.130801756532968	30.561769779479103	22.474523256629695
41	21.216364356581575	25.685881432843743	30.567391516421022	22.530362694153652
42	19.758846381786327	26.85454133594673	30.7342012066893	22.65241107557764
43	20.138856902465463	26.510906760185915	30.613924381024322	22.7363119563243
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	431.0
1	604.0
2	777.0
3	1962.0
4	3147.0
5	3147.0
6	5017.0
7	6887.0
8	7218.5
9	7550.0
10	10919.0
11	14288.0
12	14288.0
13	25088.0
14	35888.0
15	56923.5
16	77959.0
17	77824.5
18	77690.0
19	77690.0
20	85634.0
21	93578.0
22	82436.0
23	71294.0
24	80760.0
25	90226.0
26	90226.0
27	101489.5
28	112753.0
29	120997.0
30	129241.0
31	141422.0
32	153603.0
33	153603.0
34	167169.0
35	180735.0
36	194181.0
37	207627.0
38	216749.0
39	225871.0
40	225871.0
41	235665.5
42	245460.0
43	262213.5
44	278967.0
45	318619.5
46	358272.0
47	358272.0
48	361316.5
49	364361.0
50	337179.0
51	309997.0
52	295011.0
53	280025.0
54	280025.0
55	254581.0
56	229137.0
57	202449.5
58	175762.0
59	158707.0
60	141652.0
61	141652.0
62	124905.5
63	108159.0
64	94358.0
65	80557.0
66	68986.5
67	57416.0
68	57416.0
69	49330.0
70	41244.0
71	35754.5
72	30265.0
73	24298.5
74	18332.0
75	18332.0
76	14517.0
77	10702.0
78	8341.0
79	5980.0
80	4934.0
81	3888.0
82	3888.0
83	2828.0
84	1768.0
85	1380.5
86	993.0
87	821.5
88	650.0
89	650.0
90	494.5
91	339.0
92	198.0
93	57.0
94	41.5
95	26.0
96	26.0
97	19.0
98	12.0
99	6.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	4233567.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.79371977344156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.98032612790247	30.375121857449507
2	14.606849158911164	12.5016281935875
3	5.76800606607349	7.405033057291799
4	2.760221678456504	4.724806120817846
5	1.5723431052832062	3.364320511759622
6	0.9786644078428177	2.512841425288
7	0.6476174564209366	1.9399771965326595
8	0.4476037466989793	1.532370344462291
9	0.321667001279824	1.238879476181885
>10	1.6709532255663146	13.270465310657015
>50	0.14314788307910098	4.2426229109566265
>100	0.09272882426307677	7.454059170697651
>500	0.004852938616122988	1.38743616259165
>1k	0.0042463212049960305	3.419208471747158
>5k	4.963232577268082E-4	1.4919345713868475
>10k+	2.757351431815601E-4	3.139295218591943
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	37133	0.8771090666570294	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	35326	0.8344263832366419	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32064	0.7573755180914817	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	15908	0.37575878685751285	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12593	0.2974560223093198	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8595	0.20302028998241908	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	8195	0.19357199260103833	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8103	0.19139888420332074	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	8047	0.19007612256992742	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	6555	0.1548339733373772	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	6516	0.15391276434269258	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	6059	0.14311808458446507	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	6011	0.14198428889869938	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5138	0.12136337986383586	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4767	0.11260008404260521	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4592	0.10846645393825112	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4571	0.10797041832572864	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	4293	0.10140385164566901	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.36207434534519E-5	0.0
3	0.0	0.0	0.0	2.36207434534519E-5	0.0
4	0.0	0.0	0.0	4.72414869069038E-5	0.0
5	0.0	0.0	0.0	4.72414869069038E-5	0.0
6	0.0	0.0	0.0	4.72414869069038E-5	0.0
7	0.0	0.0	0.0	4.72414869069038E-5	0.0
8	0.0	0.0	0.0	4.72414869069038E-5	0.0
9	0.0	0.0	0.0	7.08622303603557E-5	0.0
10	0.0	0.0	0.0	1.417244607207114E-4	0.0
11	0.0	0.0	0.0	1.889659476276152E-4	0.0
12	0.0	0.0	0.0	3.306904083483266E-4	0.0
13	0.0	0.0	0.0	3.543111518017785E-4	0.0
14	0.0	0.0	0.0	4.487941256155861E-4	0.0
15	0.0	0.0	0.0	5.668978428828456E-4	0.0
16	0.0	0.0	0.0	9.212089946846241E-4	0.0
17	0.0	0.0	0.0	0.0013700031203002102	0.0
18	0.0	0.0	0.0	0.0016062105548347292	0.0
19	0.0	0.0	0.0	0.0020313839369968632	0.0
20	0.0	0.0	0.0	0.0027163854971469685	0.0
21	0.0	0.0	0.0	0.0036612152352850447	0.0
22	0.0	0.0	0.0	0.005928806606816427	0.0
23	0.0	0.0	0.0	0.009849850020089443	0.0
24	0.0	0.0	0.0	0.01613296777870765	0.0
25	0.0	0.0	0.0	0.020691771265223865	0.0
26	0.0	0.0	0.0	0.02893541073047858	0.0
27	0.0	0.0	0.0	0.09228624467263657	0.0
28	0.0	0.0	0.0	0.15636932166185158	0.0
29	0.0	0.0	0.0	0.22789293283890394	0.0
30	0.0	0.0	0.0	0.34932717493310017	0.0
31	0.0	0.0	0.0	0.5427810638168712	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8205	0.0	29.2663	1
GTATCAA	14160	0.0	16.932203	2
TTGGACC	1405	0.0	16.72242	4
GTATTGG	970	0.0	16.592783	1
ACGAACG	195	1.8189894E-12	16.128204	15
ATTGGAC	870	0.0	15.948275	3
GGACCCT	1625	0.0	15.36923	6
ATTAACG	110	1.4536203E-5	15.136364	3
GCGCTAT	155	1.2125201E-7	14.322581	19
TCCGATA	240	5.456968E-12	13.875001	8
CCGATAA	230	4.0017767E-11	13.673913	9
GACCCTC	1655	0.0	13.4138975	7
TATTGGA	1285	0.0	13.245136	2
TGGACCC	1800	0.0	13.155556	5
CTGTGCG	430	0.0	12.906977	9
CGAACGA	245	1.1459633E-10	12.836734	16
ACGGTGT	305	0.0	12.737704	29
CTAATAC	575	0.0	12.547826	3
TATCAAC	21250	0.0	12.336236	1
TGTGCGC	420	0.0	12.333333	10
>>END_MODULE