FastQCFastQC Report
Wed 25 May 2016
SRR2088400_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088400_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412206
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT150551.0660625999323043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT140080.9919232746497324No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT128330.9087201159037704No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG64040.45347491796522604No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49490.35044462351809863No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT36030.2551327497546392No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT34680.24557323789872015No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA34460.24401539152220003No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32300.22872017255272958No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT27160.1923232163013045No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA27000.19119023711838073No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG25490.18049774607953797No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT24190.1712922902182826No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA20700.14657918179075857No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19500.13808183791883052No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT19070.135036956364723No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18940.13411641077859746No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT17770.12583150050346764No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA16120.11414765267956659No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33250.028.987971
TGCGACG809.786163E-1025.43750222
ACGTTTA903.8307917E-922.61111326
GTACTAG1400.022.4642851
ATTGGAC3750.022.23
CGTTTAG502.7025255E-422.226
TAAACGT909.48221E-820.5555574
GTATTGG4600.020.1086941
CAATGCG1052.2604581E-819.38095319
CGACGAG1409.458745E-1118.524
CGGTATA609.238244E-418.528
GCGACGA1401.869921E-917.17857223
GTATCAA56300.017.0870342
ACGTGAT1201.0416625E-716.9583327
GACCCTC6000.016.657
GTATAGA1352.2204404E-816.4444431
GGACCCT6250.016.286
AGCCCGT803.3832496E-416.18756
CGGACCA1151.2426117E-616.0869569
CGAACGA700.002592887715.85714216
Produced by FastQC (version 0.11.5)