##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088400_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1412206
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39421302557842	31.0	31.0	34.0	30.0	34.0
2	31.591022839444104	31.0	31.0	34.0	30.0	34.0
3	31.675878731573157	31.0	31.0	34.0	30.0	34.0
4	35.46369226585923	37.0	35.0	37.0	33.0	37.0
5	35.282687511595334	37.0	35.0	37.0	33.0	37.0
6	35.31497387774871	37.0	35.0	37.0	32.0	37.0
7	35.2000933291602	37.0	35.0	37.0	32.0	37.0
8	35.234550766672854	37.0	35.0	37.0	32.0	37.0
9	36.74093227192067	39.0	35.0	39.0	32.0	39.0
10	36.60672451469545	39.0	35.0	39.0	32.0	39.0
11	36.70968541416762	39.0	35.0	39.0	32.0	39.0
12	36.57341634294147	39.0	35.0	39.0	32.0	39.0
13	36.61675562913626	39.0	35.0	39.0	32.0	39.0
14	37.63127121680548	39.0	36.0	41.0	32.0	41.0
15	37.62093561420926	39.0	36.0	41.0	32.0	41.0
16	37.57492957826266	39.0	36.0	41.0	32.0	41.0
17	37.6231024368966	39.0	36.0	41.0	32.0	41.0
18	37.60173374139467	39.0	36.0	41.0	32.0	41.0
19	37.66263349681279	39.0	37.0	41.0	32.0	41.0
20	37.628540028862645	39.0	37.0	41.0	32.0	41.0
21	37.57970225307073	39.0	36.0	41.0	32.0	41.0
22	37.4746283474224	39.0	36.0	41.0	32.0	41.0
23	37.367651744858755	39.0	36.0	41.0	32.0	41.0
24	37.30141778182503	39.0	36.0	41.0	31.0	41.0
25	37.24237327981895	39.0	36.0	41.0	31.0	41.0
26	37.01670365371624	39.0	36.0	41.0	31.0	41.0
27	36.85298745367177	39.0	35.0	40.0	31.0	41.0
28	36.746022180899956	39.0	35.0	40.0	30.0	41.0
29	36.60302250521524	39.0	35.0	40.0	30.0	41.0
30	36.43368885275944	38.0	35.0	40.0	30.0	41.0
31	36.2034830612531	38.0	35.0	40.0	30.0	41.0
32	36.06738818557633	38.0	35.0	40.0	30.0	41.0
33	36.14824890986159	38.0	35.0	40.0	30.0	41.0
34	36.12207071772815	38.0	35.0	40.0	30.0	41.0
35	36.058662121531846	38.0	35.0	40.0	30.0	41.0
36	35.98446614729013	38.0	35.0	40.0	29.0	41.0
37	35.86285216179509	38.0	35.0	40.0	29.0	41.0
38	35.762800894487064	38.0	35.0	40.0	28.0	41.0
39	35.710177552000204	38.0	35.0	40.0	27.0	41.0
40	35.56744200208752	38.0	34.0	40.0	27.0	41.0
41	35.43894516805622	38.0	34.0	40.0	26.0	41.0
42	35.29018783378629	38.0	34.0	40.0	25.0	41.0
43	34.95611121890149	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	2.0
15	5.0
16	13.0
17	29.0
18	94.0
19	171.0
20	403.0
21	873.0
22	1491.0
23	2674.0
24	4444.0
25	7022.0
26	10790.0
27	15753.0
28	22789.0
29	30851.0
30	39755.0
31	49188.0
32	60540.0
33	73839.0
34	91151.0
35	111559.0
36	141107.0
37	192177.0
38	256878.0
39	298606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.01519183461903	22.244417599132138	13.52649684252864	23.213893723720194
2	18.11874471571428	23.05301067974502	35.78897129738862	23.039273307152072
3	19.733664918574203	25.9720607333491	31.793236964012333	22.501037384064364
4	13.202818852207116	16.330266264270225	36.060744678892455	34.40617020463021
5	12.040028154532695	39.60279166070673	34.754419680981385	13.602760503779193
6	31.501069957215872	36.744355993389064	15.908231518631135	15.846342530763927
7	25.200501909778033	34.30575992454359	22.07871939362954	18.41501877204884
8	27.06779322563422	33.76978995982173	20.568316520394333	18.594100294149722
9	25.72138908912722	13.821637919680274	22.858350693878936	37.59862229731357
10	16.316741325274077	27.364350526764508	34.144097957380154	22.17481019058126
11	32.31752308091029	22.23797378002926	24.487928814917936	20.95657432414251
12	20.182041430216273	27.21132752587087	31.67689416416585	20.929736879747004
13	30.467014019201166	21.01223192650364	28.001084827567652	20.519669226727547
14	21.034820698963184	22.4693139669425	28.142990470228845	28.35287486386547
15	23.34354902896603	29.09157729113175	25.964908802256893	21.59996487764533
16	22.21056984604229	28.220599544259123	26.766845630170106	22.801984979528484
17	20.14139580202888	27.286033340744904	27.827101711789926	24.74546914543629
18	21.02412820792434	26.484875436019955	31.106722390359483	21.384273965696224
19	20.511101071656686	25.52049771775506	32.22206958474897	21.74633162583929
20	23.81012401873381	24.665452490642302	31.158626999177173	20.365796491446716
21	23.32804137639976	25.411519282597585	30.212801815032652	21.047637525970007
22	22.33073645063114	25.05781734392858	30.98577686258237	21.62566934285791
23	21.426619062658	26.390696541439425	31.64552480303865	20.53715959286393
24	20.490211767971527	25.947914114513036	30.512687242512776	23.049186875002654
25	21.497855128784327	26.354016340392267	31.378566583062245	20.76956194776116
26	22.30177467026765	25.922351271698325	30.250048505671266	21.525825552362758
27	21.451615415881253	26.878656513284888	30.01594668199965	21.653781388834208
28	21.07334199118259	26.531823260912358	30.23291219552955	22.161922552375504
29	20.393058803035817	26.474041322583247	31.03463658984596	22.098263284534976
30	20.12086055433839	26.675145127552213	32.21477603125889	20.9892182868505
31	21.095010218056007	26.225564825528284	31.375380079110272	21.304044877305437
32	21.817709314363483	26.354936885978393	29.88501677517303	21.942337024485095
33	22.36769989647403	26.23958544291697	29.730860795096465	21.66185386551254
34	21.293139952669797	28.121888732946893	29.717902345691776	20.867068968691537
35	20.48086468971241	28.65169812336161	29.957173386885483	20.910263800040504
36	21.02752714547311	26.293118709310114	31.277235757389505	21.402118387827272
37	21.09040749012538	26.242913569266808	30.800676388572207	21.86600255203561
38	21.073271179983657	26.011290137557836	31.22533114857181	21.6901075338867
39	21.939433765328854	25.577713166492703	31.23276632445975	21.250086743718693
40	20.647412629602197	26.00059764651899	30.801738556556195	22.550251167322614
41	21.03581205574824	25.74688112074301	30.88260494573738	22.334701877771373
42	19.473079706501743	26.913283189562996	31.054534536746054	22.559102567189207
43	19.80412206151227	26.54810983666689	30.825743552994396	22.822024548826448
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	152.0
1	196.0
2	240.0
3	636.0
4	1032.0
5	1032.0
6	1692.0
7	2352.0
8	2497.0
9	2642.0
10	3978.5
11	5315.0
12	5315.0
13	9351.0
14	13387.0
15	21556.5
16	29726.0
17	29419.5
18	29113.0
19	29113.0
20	31854.5
21	34596.0
22	28851.0
23	23106.0
24	26216.0
25	29326.0
26	29326.0
27	32451.5
28	35577.0
29	37904.0
30	40231.0
31	43377.5
32	46524.0
33	46524.0
34	51132.5
35	55741.0
36	60421.0
37	65101.0
38	68360.0
39	71619.0
40	71619.0
41	75296.5
42	78974.0
43	86643.0
44	94312.0
45	110589.0
46	126866.0
47	126866.0
48	128359.0
49	129852.0
50	119580.0
51	109308.0
52	103621.0
53	97934.0
54	97934.0
55	87144.0
56	76354.0
57	66664.5
58	56975.0
59	50760.0
60	44545.0
61	44545.0
62	39796.5
63	35048.0
64	30579.5
65	26111.0
66	22216.0
67	18321.0
68	18321.0
69	15285.5
70	12250.0
71	10386.5
72	8523.0
73	6718.0
74	4913.0
75	4913.0
76	3770.5
77	2628.0
78	2072.0
79	1516.0
80	1196.5
81	877.0
82	877.0
83	671.5
84	466.0
85	367.0
86	268.0
87	244.0
88	220.0
89	220.0
90	175.0
91	130.0
92	76.0
93	22.0
94	16.0
95	10.0
96	10.0
97	6.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1412206.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.46300575201248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.48720654358715	40.90650567564029
2	11.785984941886115	12.130844217148349
3	3.7332076629540834	5.763662622961892
4	1.5402757832430507	3.170688859708906
5	0.8260720429037123	2.125607514776522
6	0.49538484850350595	1.5296395984797453
7	0.33001645887514414	1.1888547244945231
8	0.24723113036113917	1.0178605667081482
9	0.1899942963594844	0.8799909809757938
>10	1.165134583374371	11.627518221241802
>50	0.12330428567171793	4.371159467074911
>100	0.06597392126342344	5.983741274864615
>500	0.0064869533487358355	2.3078868327188182
>1k	0.003174466532360089	3.5653200655029265
>5k	1.3802028401565606E-4	0.4548721924391023
>10k+	4.140608520469682E-4	2.975847185263684
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15055	1.0660625999323043	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14008	0.9919232746497324	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12833	0.9087201159037704	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6404	0.45347491796522604	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4949	0.35044462351809863	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	3603	0.2551327497546392	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	3468	0.24557323789872015	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3446	0.24401539152220003	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3230	0.22872017255272958	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	2716	0.1923232163013045	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	2700	0.19119023711838073	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	2549	0.18049774607953797	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	2419	0.1712922902182826	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2070	0.14657918179075857	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1950	0.13808183791883052	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1907	0.135036956364723	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1894	0.13411641077859746	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	1777	0.12583150050346764	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1612	0.11414765267956659	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	7.08111989327336E-5	0.0	0.0
5	0.0	0.0	7.08111989327336E-5	0.0	0.0
6	0.0	0.0	7.08111989327336E-5	0.0	0.0
7	0.0	0.0	7.08111989327336E-5	0.0	0.0
8	0.0	0.0	7.08111989327336E-5	0.0	0.0
9	0.0	0.0	7.08111989327336E-5	0.0	0.0
10	0.0	0.0	1.416223978654672E-4	1.416223978654672E-4	0.0
11	0.0	0.0	1.416223978654672E-4	2.1243359679820083E-4	0.0
12	0.0	0.0	1.416223978654672E-4	4.2486719359640166E-4	0.0
13	0.0	0.0	1.416223978654672E-4	4.956783925291352E-4	0.0
14	7.08111989327336E-5	0.0	1.416223978654672E-4	6.373007903946025E-4	0.0
15	7.08111989327336E-5	0.0	1.416223978654672E-4	0.0011329791829237377	0.0
16	7.08111989327336E-5	0.0	1.416223978654672E-4	0.0018410911722510739	0.0
17	7.08111989327336E-5	0.0	1.416223978654672E-4	0.0024075807637129426	0.0
18	7.08111989327336E-5	0.0	1.416223978654672E-4	0.0028324479573093443	0.0
19	7.08111989327336E-5	0.0	1.416223978654672E-4	0.003257315150905746	0.0
20	7.08111989327336E-5	0.0	1.416223978654672E-4	0.004107049538098549	0.0
21	7.08111989327336E-5	0.0	1.416223978654672E-4	0.0058773295114168895	0.0
22	7.08111989327336E-5	0.0	1.416223978654672E-4	0.010126001447380906	0.0
23	7.08111989327336E-5	0.0	1.416223978654672E-4	0.016923876544923333	0.0
24	7.08111989327336E-5	0.0	1.416223978654672E-4	0.02627095480404417	0.0
25	7.08111989327336E-5	0.0	2.832447957309344E-4	0.031652605922931926	0.0
26	7.08111989327336E-5	0.0	2.832447957309344E-4	0.04036238339165816	0.0
27	7.08111989327336E-5	0.0	3.5405599466366804E-4	0.10904924635640977	0.0
28	7.08111989327336E-5	0.0	3.5405599466366804E-4	0.17122147901934986	0.0
29	7.08111989327336E-5	0.0	4.2486719359640166E-4	0.24387376912433456	0.0
30	7.08111989327336E-5	0.0	4.2486719359640166E-4	0.37912315908585575	0.0
31	7.08111989327336E-5	0.0	4.2486719359640166E-4	0.5836259016035904	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3325	0.0	28.98797	1
TGCGACG	80	9.786163E-10	25.437502	22
ACGTTTA	90	3.8307917E-9	22.611113	26
GTACTAG	140	0.0	22.464285	1
ATTGGAC	375	0.0	22.2	3
CGTTTAG	50	2.7025255E-4	22.2	26
TAAACGT	90	9.48221E-8	20.555557	4
GTATTGG	460	0.0	20.108694	1
CAATGCG	105	2.2604581E-8	19.380953	19
CGACGAG	140	9.458745E-11	18.5	24
CGGTATA	60	9.238244E-4	18.5	28
GCGACGA	140	1.869921E-9	17.178572	23
GTATCAA	5630	0.0	17.087034	2
ACGTGAT	120	1.0416625E-7	16.958332	7
GACCCTC	600	0.0	16.65	7
GTATAGA	135	2.2204404E-8	16.444443	1
GGACCCT	625	0.0	16.28	6
AGCCCGT	80	3.3832496E-4	16.1875	6
CGGACCA	115	1.2426117E-6	16.086956	9
CGAACGA	70	0.0025928877	15.857142	16
>>END_MODULE