FastQCFastQC Report
Wed 25 May 2016
SRR2088399_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088399_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725578
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70950.9778411142564962No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT68250.9406294016632257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT62280.8583501704847722No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG27900.38452103013046146No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23180.31946944367111463No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT15250.21017726557310173No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT15060.2075586635757975No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA14480.19956503642613196No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13130.18095918012949677No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT12130.1671770643542114No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA11670.16083729109758013No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT11630.16028600646656874No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG10960.15105198889712754No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA8720.12018004956048833No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT8640.1190774802984655No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT8230.11342681283059851No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA7980.10998128388677716No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA7890.10874089346700147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACA250.00549502529.5999982
GGTCTAT451.3225556E-424.6666641
GGTATCA17200.024.6308141
TATACCG400.001930504723.1255
CTAACCC450.003824469920.5555534
GTCTATA609.2323404E-418.52
GTCGTAG500.007032444218.49999811
CTATACT953.6026795E-617.5263164
GTCTAGG852.720875E-517.4117641
CGTCTGT1401.8644641E-917.17857234
GTATTGG1352.2151653E-816.4444451
TTAGACT957.053939E-515.5789484
TCTATAC957.053939E-515.5789483
CTAGAGT1101.4503223E-515.1363644
CAAGACT1601.0939402E-815.031254
GTATCAA28200.015.023052
GGTCGGC750.00410246714.822
CGCAAGA750.00410246714.82
GTAACAC750.00410246714.83
TCTAGAC1001.09248154E-414.7999993
Produced by FastQC (version 0.11.5)