##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088399_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	725578
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35141914446138	31.0	31.0	34.0	30.0	34.0
2	31.588767851285457	31.0	31.0	34.0	30.0	34.0
3	31.686069588658974	31.0	31.0	34.0	30.0	34.0
4	35.47429359765594	37.0	35.0	37.0	33.0	37.0
5	35.27847454029753	37.0	35.0	37.0	33.0	37.0
6	35.31464845957292	37.0	35.0	37.0	32.0	37.0
7	35.19418725485062	37.0	35.0	37.0	32.0	37.0
8	35.20260123653143	37.0	35.0	37.0	32.0	37.0
9	36.71211778747426	39.0	35.0	39.0	32.0	39.0
10	36.59029904434809	39.0	35.0	39.0	32.0	39.0
11	36.70212988817191	39.0	35.0	39.0	32.0	39.0
12	36.56193958471729	39.0	35.0	39.0	32.0	39.0
13	36.60173957865316	39.0	35.0	39.0	32.0	39.0
14	37.60932112054114	39.0	36.0	41.0	32.0	41.0
15	37.60962294887662	39.0	36.0	41.0	32.0	41.0
16	37.574533682112744	39.0	36.0	41.0	32.0	41.0
17	37.60622703554959	39.0	36.0	41.0	32.0	41.0
18	37.57815562213849	39.0	36.0	41.0	32.0	41.0
19	37.65068676282908	39.0	37.0	41.0	32.0	41.0
20	37.61400152705843	39.0	36.0	41.0	32.0	41.0
21	37.550718186053054	39.0	36.0	41.0	32.0	41.0
22	37.44771892201803	39.0	36.0	41.0	32.0	41.0
23	37.35769276356229	39.0	36.0	41.0	31.0	41.0
24	37.283691071118476	39.0	36.0	41.0	31.0	41.0
25	37.21812265531755	39.0	36.0	41.0	31.0	41.0
26	36.99656411853722	39.0	36.0	41.0	31.0	41.0
27	36.8425985903652	39.0	35.0	40.0	30.0	41.0
28	36.751861274735454	39.0	35.0	40.0	30.0	41.0
29	36.61947578344437	39.0	35.0	40.0	30.0	41.0
30	36.44305229761652	38.0	35.0	40.0	30.0	41.0
31	36.206919173403826	38.0	35.0	40.0	30.0	41.0
32	36.0543580428293	38.0	35.0	40.0	30.0	41.0
33	36.142799257970886	38.0	35.0	40.0	30.0	41.0
34	36.08963750279088	38.0	35.0	40.0	30.0	41.0
35	36.05482525655408	38.0	35.0	40.0	30.0	41.0
36	35.98812670725959	38.0	35.0	40.0	29.0	41.0
37	35.87898475422353	38.0	35.0	40.0	29.0	41.0
38	35.7618739818462	38.0	35.0	40.0	28.0	41.0
39	35.72089837343469	38.0	35.0	40.0	27.0	41.0
40	35.56649457398102	38.0	34.0	40.0	27.0	41.0
41	35.44356361411178	38.0	34.0	40.0	26.0	41.0
42	35.32295769717384	38.0	34.0	40.0	25.0	41.0
43	34.98279165024298	38.0	34.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	4.0
17	13.0
18	31.0
19	89.0
20	213.0
21	421.0
22	771.0
23	1366.0
24	2193.0
25	3649.0
26	5616.0
27	8182.0
28	11789.0
29	15845.0
30	20433.0
31	25377.0
32	31129.0
33	38223.0
34	47392.0
35	57423.0
36	72793.0
37	98365.0
38	130780.0
39	153480.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.231266659132444	21.92913787352979	13.333645727957574	23.505949739380192
2	17.9812508096993	22.730154442389377	35.93976112836938	23.348833619541935
3	19.595412209300722	25.76097952253238	31.58599075495674	23.057617513210158
4	13.284994859270816	16.26510175336077	35.84480235067767	34.60510103669075
5	12.315836477952748	39.3698541025224	34.561273908525344	13.753035510999506
6	31.756337705939263	36.60447257221139	15.992078039852368	15.647111681996972
7	25.50945590963342	33.87561364870489	22.255774017404057	18.359156424257623
8	27.16069120067036	33.82572238959836	20.497176044477644	18.516410365253634
9	25.91106676332524	13.809542185678175	22.581583234331802	37.697807816664785
10	16.349034838432257	27.50676012778778	33.95789287988335	22.18631215389662
11	32.483757776558825	22.381742555590165	24.184029835524232	20.950469832326778
12	20.405524974571996	27.089851125585398	31.599772870732025	20.904851029110585
13	30.474187475364467	21.186833117872926	27.46059003994057	20.878389366822038
14	21.089669201657163	22.250536813409447	28.077064078569087	28.582729906364307
15	23.364545231525764	28.9037153827707	25.76869750736654	21.963041878336995
16	22.263216359922712	28.05280755480458	26.79257088831249	22.891405196960214
17	20.495246548269105	27.240489651009263	27.692680869596376	24.571582931125256
18	21.259327046850924	26.618502766070634	30.74955414855467	21.37261603852377
19	20.961771167262512	25.603312118063116	31.79561673589883	21.639299978775544
20	23.75940284848769	24.96065205946156	30.777256201262993	20.502688890787756
21	23.303352637483496	25.603312118063116	29.91987077888249	21.173464465570895
22	22.398005452205	25.341727560648202	30.52352744983999	21.73673953730681
23	21.591200394719795	26.417421696909223	31.02698813911116	20.964389769259817
24	20.79252678554201	26.168103222534313	30.203506721537863	22.83586327038582
25	21.643848076981385	26.580601947688603	30.741147057931745	21.034402917398268
26	22.41537091808186	26.00189090628437	29.917803461516197	21.664934714117575
27	21.590097825457775	26.846458960993857	29.713276863410965	21.850166350137407
28	21.326445950676565	26.654336267086375	29.827117139714822	22.192100642522234
29	20.63651323496578	26.70216020882662	30.57080010694922	22.090526449258384
30	20.456932266413812	27.035825231746273	31.454647191618267	21.052595310221644
31	21.30356763848959	26.50025221271869	30.7897979266185	21.40638222217322
32	21.787595544517615	26.67087480601672	29.617766801088237	21.923762848377432
33	22.324271132807226	26.48839959315194	29.443009573057616	21.744319700983215
34	21.383503909986246	28.022624721256705	29.595991058163285	20.99788031059376
35	20.587586723963515	28.429197136627625	29.74745651053367	21.235759628875186
36	21.04708246391153	26.428447389529452	30.889993908304824	21.63447623825419
37	21.164506090316962	26.40474215039596	30.454616870963562	21.976134888323514
38	21.22101276499563	26.251071559501526	30.77229463958389	21.755621035918953
39	21.76816276127446	25.83581641119218	30.963314764229345	21.432706063304014
40	20.724443133612098	26.08348103167406	30.629787562467442	22.562288272246402
41	21.164230448001454	25.619575014677952	30.718544388060277	22.497650149260316
42	19.607953934656233	26.991033355476603	30.790073568934005	22.61093914093316
43	19.995507030257258	26.54973000835196	30.578655912941134	22.87610704844965
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	65.0
1	98.5
2	132.0
3	346.5
4	561.0
5	561.0
6	936.0
7	1311.0
8	1393.5
9	1476.0
10	2165.0
11	2854.0
12	2854.0
13	4844.0
14	6834.0
15	10859.5
16	14885.0
17	14842.0
18	14799.0
19	14799.0
20	15798.5
21	16798.0
22	14336.0
23	11874.0
24	13426.0
25	14978.0
26	14978.0
27	16683.0
28	18388.0
29	19611.0
30	20834.0
31	22736.5
32	24639.0
33	24639.0
34	26763.5
35	28888.0
36	31518.5
37	34149.0
38	35690.5
39	37232.0
40	37232.0
41	38764.0
42	40296.0
43	43667.5
44	47039.0
45	54859.0
46	62679.0
47	62679.0
48	63583.0
49	64487.0
50	59290.0
51	54093.0
52	51115.5
53	48138.0
54	48138.0
55	43492.0
56	38846.0
57	34705.5
58	30565.0
59	27562.0
60	24559.0
61	24559.0
62	21847.5
63	19136.0
64	16938.5
65	14741.0
66	12638.5
67	10536.0
68	10536.0
69	8985.0
70	7434.0
71	6260.0
72	5086.0
73	4136.0
74	3186.0
75	3186.0
76	2518.5
77	1851.0
78	1432.5
79	1014.0
80	781.0
81	548.0
82	548.0
83	414.0
84	280.0
85	232.5
86	185.0
87	145.5
88	106.0
89	106.0
90	82.5
91	59.0
92	34.5
93	10.0
94	8.0
95	6.0
96	6.0
97	3.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	725578.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.60804011809009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.1184470075517	51.823726579126806
2	9.623013844609602	11.857100459912894
3	2.5825046078200797	4.77309142451196
4	1.1031075294720831	2.7184117172113336
5	0.6229936116053366	1.9190707708547707
6	0.3771102666045253	1.3939814660349148
7	0.2607876872862439	1.1246632810444392
8	0.1998497184393183	0.9849879576958825
9	0.1478028009906832	0.8195256803700085
>10	0.8652449443448621	9.914355079891461
>50	0.06145672211424547	2.6354324187019498
>100	0.03207392892956458	3.9778639558879307
>500	0.0026915185112828493	1.1264649656647208
>1k	0.002242932092735708	2.1472202038106105
>5k	6.728796278207123E-4	2.784104039280274
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7095	0.9778411142564962	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6825	0.9406294016632257	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6228	0.8583501704847722	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2790	0.38452103013046146	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2318	0.31946944367111463	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	1525	0.21017726557310173	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	1506	0.2075586635757975	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1448	0.19956503642613196	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1313	0.18095918012949677	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	1213	0.1671770643542114	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	1167	0.16083729109758013	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	1163	0.16028600646656874	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	1096	0.15105198889712754	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	872	0.12018004956048833	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	864	0.1190774802984655	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	823	0.11342681283059851	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	798	0.10998128388677716	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	789	0.10874089346700147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.3782115775285358E-4	0.0
3	0.0	0.0	0.0	1.3782115775285358E-4	0.0
4	0.0	0.0	0.0	1.3782115775285358E-4	0.0
5	0.0	0.0	0.0	1.3782115775285358E-4	0.0
6	0.0	0.0	0.0	1.3782115775285358E-4	0.0
7	0.0	0.0	0.0	1.3782115775285358E-4	0.0
8	0.0	0.0	0.0	1.3782115775285358E-4	0.0
9	0.0	0.0	0.0	1.3782115775285358E-4	0.0
10	0.0	0.0	0.0	1.3782115775285358E-4	0.0
11	0.0	0.0	0.0	1.3782115775285358E-4	0.0
12	0.0	0.0	0.0	4.1346347325856074E-4	0.0
13	0.0	0.0	0.0	5.512846310114143E-4	0.0
14	0.0	0.0	0.0	8.269269465171215E-4	0.0
15	0.0	0.0	0.0	0.0012403904197756824	0.0
16	1.3782115775285358E-4	0.0	0.0	0.0028942443128099253	0.0
17	1.3782115775285358E-4	0.0	0.0	0.0042724558903384615	0.0
18	1.3782115775285358E-4	0.0	0.0	0.0045480982058441685	0.0
19	1.3782115775285358E-4	0.0	0.0	0.005237203994608437	0.0
20	1.3782115775285358E-4	0.0	0.0	0.006339773256631265	0.0
21	1.3782115775285358E-4	0.0	0.0	0.008269269465171215	0.0
22	1.3782115775285358E-4	0.0	0.0	0.011990440724498262	0.0
23	1.3782115775285358E-4	0.0	0.0	0.018743677454388086	0.0
24	1.3782115775285358E-4	0.0	0.0	0.026461662288547887	0.0
25	1.3782115775285358E-4	0.0	0.0	0.03156104512540347	0.0
26	1.3782115775285358E-4	0.0	0.0	0.0403815992215861	0.0
27	1.3782115775285358E-4	0.0	0.0	0.09730173737351464	0.0
28	1.3782115775285358E-4	0.0	0.0	0.16166421804409725	0.0
29	1.3782115775285358E-4	0.0	0.0	0.24187613185625806	0.0
30	1.3782115775285358E-4	0.0	0.0	0.37790561455832455	0.0
31	1.3782115775285358E-4	0.0	0.0	0.6015893535912059	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTACA	25	0.005495025	29.599998	2
GGTCTAT	45	1.3225556E-4	24.666664	1
GGTATCA	1720	0.0	24.630814	1
TATACCG	40	0.0019305047	23.125	5
CTAACCC	45	0.0038244699	20.555553	4
GTCTATA	60	9.2323404E-4	18.5	2
GTCGTAG	50	0.0070324442	18.499998	11
CTATACT	95	3.6026795E-6	17.526316	4
GTCTAGG	85	2.720875E-5	17.411764	1
CGTCTGT	140	1.8644641E-9	17.178572	34
GTATTGG	135	2.2151653E-8	16.444445	1
TTAGACT	95	7.053939E-5	15.578948	4
TCTATAC	95	7.053939E-5	15.578948	3
CTAGAGT	110	1.4503223E-5	15.136364	4
CAAGACT	160	1.0939402E-8	15.03125	4
GTATCAA	2820	0.0	15.02305	2
GGTCGGC	75	0.004102467	14.8	22
CGCAAGA	75	0.004102467	14.8	2
GTAACAC	75	0.004102467	14.8	3
TCTAGAC	100	1.09248154E-4	14.799999	3
>>END_MODULE