FastQCFastQC Report
Wed 25 May 2016
SRR2088398_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088398_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1818297
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT117560.646539041751705No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT115190.633504867466646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT98290.5405607554761406No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44030.24214965981905046No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG27760.1526703283347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34600.023.04481
GGACCGT904.4482465E-516.4444456
TACGTTA1504.6748028E-916.03333319
CGCATAC855.366283E-415.23529437
ATAGTCG855.366283E-415.23529420
GTATCAA53100.014.9463282
TAACGTG750.00410562714.85
GGCTAGA1752.240995E-914.81
ATTACGT1651.6467311E-814.57575717
CGACGAG1152.2111315E-514.47826124
CGAACGT908.2772796E-414.3888884
TAGAACA1803.3342076E-914.3888884
GACGGTA2101.364242E-1014.0952398
ACGTTTA1854.9021764E-914.026
ATTCGTC1356.5715267E-613.70370417
TACGACG2202.8376235E-1013.4545455
GTACTAG1102.4575918E-413.4545451
GTATTAG2900.013.3965511
TAACGCT1805.1632014E-813.36111132
CAAGACG3600.013.3611114
Produced by FastQC (version 0.11.5)