##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088398_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1818297
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35728211617794	31.0	31.0	34.0	30.0	34.0
2	31.594649278968177	31.0	31.0	34.0	30.0	34.0
3	31.694229270575708	31.0	31.0	34.0	30.0	34.0
4	35.48068054888723	37.0	35.0	37.0	33.0	37.0
5	35.267175274446366	37.0	35.0	37.0	33.0	37.0
6	35.299599020402056	37.0	35.0	37.0	32.0	37.0
7	35.181902076503455	37.0	35.0	37.0	32.0	37.0
8	35.18743527597527	37.0	35.0	37.0	32.0	37.0
9	36.73614211539699	39.0	35.0	39.0	32.0	39.0
10	36.57227669627129	39.0	35.0	39.0	32.0	39.0
11	36.687818876674164	39.0	35.0	39.0	32.0	39.0
12	36.55348053700798	39.0	35.0	39.0	32.0	39.0
13	36.607968885171125	39.0	35.0	39.0	32.0	39.0
14	37.64907383117279	39.0	36.0	41.0	32.0	41.0
15	37.6409524956594	39.0	36.0	41.0	32.0	41.0
16	37.60859309562739	39.0	36.0	41.0	32.0	41.0
17	37.60187252137577	39.0	36.0	41.0	32.0	41.0
18	37.56191810248821	39.0	36.0	41.0	32.0	41.0
19	37.61643614876998	39.0	36.0	41.0	32.0	41.0
20	37.56649986223373	39.0	36.0	41.0	32.0	41.0
21	37.51569683060578	39.0	36.0	41.0	32.0	41.0
22	37.4135391522947	39.0	36.0	41.0	32.0	41.0
23	37.323754040181555	39.0	36.0	41.0	31.0	41.0
24	37.24721758876575	39.0	36.0	41.0	31.0	41.0
25	37.182186958456185	39.0	36.0	41.0	31.0	41.0
26	36.96704278783939	39.0	36.0	40.0	31.0	41.0
27	36.81420527009614	39.0	35.0	40.0	30.0	41.0
28	36.7360123236193	39.0	35.0	40.0	30.0	41.0
29	36.59504250405737	39.0	35.0	40.0	30.0	41.0
30	36.44723111790868	38.0	35.0	40.0	30.0	41.0
31	36.265607873741196	38.0	35.0	40.0	30.0	41.0
32	36.11601350054474	38.0	35.0	40.0	30.0	41.0
33	36.227004719251035	38.0	35.0	40.0	30.0	41.0
34	36.220721917266545	38.0	35.0	40.0	30.0	41.0
35	36.194767411484484	38.0	35.0	40.0	30.0	41.0
36	36.14280175350891	38.0	35.0	40.0	30.0	41.0
37	36.04759728471201	38.0	35.0	40.0	29.0	41.0
38	35.95294663083094	38.0	35.0	40.0	29.0	41.0
39	35.94815753422021	38.0	35.0	40.0	28.0	41.0
40	35.830066265302094	38.0	35.0	40.0	28.0	41.0
41	35.74173911082733	38.0	35.0	40.0	27.0	41.0
42	35.66324148365201	38.0	35.0	40.0	27.0	41.0
43	35.328875315748746	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	2.0
14	4.0
15	6.0
16	16.0
17	46.0
18	108.0
19	255.0
20	542.0
21	1087.0
22	1906.0
23	3356.0
24	5579.0
25	9017.0
26	13452.0
27	19988.0
28	28191.0
29	38282.0
30	49440.0
31	62640.0
32	77253.0
33	94800.0
34	117268.0
35	144506.0
36	181223.0
37	240247.0
38	334002.0
39	395080.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.88781975661842	21.85352557915456	12.967573504218507	24.291081160008513
2	18.154294925416476	23.2729306598427	34.914868143103135	23.657906271637692
3	19.25807500094869	25.792431049493015	31.526477797631518	23.423016151926777
4	13.662729466088322	16.81380984514631	36.70164995047564	32.82181073828973
5	13.063652417619343	38.59765483856597	34.72474518739238	13.613947556422302
6	31.48391049427019	37.688727419118	16.259224978097638	14.568137108514176
7	25.7301199968982	33.368311117490705	23.000422923207815	17.90114596240328
8	26.327217170792228	34.00693066094263	21.020273365682286	18.645578802582857
9	25.98167406094824	14.073993412517316	22.14803192217773	37.796300604356716
10	15.980172656062239	27.51398698892425	34.69317718722519	21.812663167788322
11	33.206291381440984	22.981064149586125	23.615393964792332	20.197250504180563
12	19.923202865098496	27.181643042913233	31.707856307302933	21.18729778468534
13	29.189180865392178	21.391114872872805	27.145950304048238	22.273753957686782
14	21.0793946203508	22.44545308054735	27.816742809343026	28.658409489758824
15	23.518380110619994	29.52966429576686	24.74106265368089	22.210892939932254
16	22.742049291177405	28.105584511221217	26.35950012566704	22.79286607193434
17	21.049971484306468	27.88691836372166	27.849300746797688	23.21380940517418
18	21.503967723644706	26.987230358956758	29.623598345044844	21.885203572353692
19	21.568808615974177	26.546873255579257	30.391019728900176	21.49329839954639
20	23.07576815008769	26.139019093140448	29.503430957648835	21.281781799123024
21	22.940586713831678	26.54912811273406	28.975299414782075	21.53498575865219
22	22.567655339034275	26.467953255161287	29.442824797049106	21.521566608755336
23	21.8286671539358	26.82383571000777	29.58092104865157	21.76657608740486
24	21.497203152180308	26.982225676003424	29.1645424262373	22.356028745578968
25	22.18746442412873	26.988330289276174	29.360165033545126	21.46404025304997
26	22.17899496066924	27.1390757395519	28.81250972750876	21.869419572270097
27	22.006305900521202	27.252863531095308	28.880485421248565	21.86034514713493
28	21.591467180554112	27.404213943046706	29.047234857671768	21.957084018727414
29	21.149020209569724	27.226685189493242	29.699493537084425	21.924801063852605
30	21.297290816626766	27.584272536334826	29.67952980178706	21.438906845251353
31	21.823772464014404	27.294165914589314	29.250776963279375	21.631284658116908
32	21.845441091306867	27.4045989186585	28.824278981926494	21.925681008108135
33	21.93316053428015	27.264522792481095	28.939441686369165	21.86287498686958
34	21.945314764309682	27.44414141364145	29.011212139710953	21.599331682337926
35	21.54955983538443	27.695090516015807	29.32419731210028	21.43115233649948
36	21.51348212090764	27.015553564681678	29.47879251849395	21.99217179591673
37	21.602576476780197	26.48912691381001	29.69520380883871	22.213092800571083
38	21.412233535005555	26.56386717901421	29.952037538421937	22.07186174755829
39	21.701625202043452	26.220193950713224	30.074844758584547	22.003336088658784
40	21.13956080882276	26.265016111229357	30.221850445774262	22.373572634173623
41	20.992720111180958	25.936466924820312	30.43523692774063	22.635576036258104
42	20.357510351719217	26.690744141358646	30.279816773607394	22.671928733314743
43	20.372689390127135	26.11905535784308	30.402733986801938	23.10552126522785
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	198.0
1	288.0
2	378.0
3	913.5
4	1449.0
5	1449.0
6	2190.5
7	2932.0
8	2996.5
9	3061.0
10	4490.0
11	5919.0
12	5919.0
13	9942.5
14	13966.0
15	20912.5
16	27859.0
17	28069.5
18	28280.0
19	28280.0
20	32090.0
21	35900.0
22	33095.0
23	30290.0
24	35286.0
25	40282.0
26	40282.0
27	45056.0
28	49830.0
29	53385.5
30	56941.0
31	62246.5
32	67552.0
33	67552.0
34	74083.0
35	80614.0
36	88286.0
37	95958.0
38	100508.0
39	105058.0
40	105058.0
41	109529.0
42	114000.0
43	118991.0
44	123982.0
45	132177.5
46	140373.0
47	140373.0
48	141236.0
49	142099.0
50	132519.0
51	122939.0
52	116776.0
53	110613.0
54	110613.0
55	103284.0
56	95955.0
57	88856.0
58	81757.0
59	75210.0
60	68663.0
61	68663.0
62	60746.0
63	52829.0
64	46123.0
65	39417.0
66	33167.0
67	26917.0
68	26917.0
69	23217.5
70	19518.0
71	16599.0
72	13680.0
73	10929.0
74	8178.0
75	8178.0
76	6359.5
77	4541.0
78	3554.0
79	2567.0
80	2073.5
81	1580.0
82	1580.0
83	1209.5
84	839.0
85	705.5
86	572.0
87	494.5
88	417.0
89	417.0
90	364.5
91	312.0
92	181.0
93	50.0
94	40.0
95	30.0
96	30.0
97	16.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1818297.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.35829529702322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.98959547481145	39.54054379193729
2	12.877825791436766	13.227663595604621
3	4.318651654026487	6.653957607974102
4	1.858508427728257	3.817992985730966
5	1.0025206518028567	2.5743875838327646
6	0.5994449622424182	1.8471882831095423
7	0.3854599102392388	1.3857594726661622
8	0.2744856091941894	1.127769037742279
9	0.2129497758062859	0.9843063722364711
>10	1.2600787792399708	12.15368793187709
>50	0.13005535354974287	4.721897648944933
>100	0.0857043672089276	7.978372843323236
>500	0.0031104099702221107	1.031789731381532
>1k	0.0012870661945746666	1.1311623583321637
>5k	1.0725551621455555E-4	0.5414265799876716
>10k+	2.145110324291111E-4	1.2820941753192654
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11756	0.646539041751705	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11519	0.633504867466646	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9829	0.5405607554761406	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4403	0.24214965981905046	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2776	0.1526703283347	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.499651597071326E-5	0.0
8	0.0	0.0	0.0	5.499651597071326E-5	0.0
9	0.0	0.0	0.0	1.0999303194142652E-4	0.0
10	0.0	0.0	0.0	2.1998606388285303E-4	0.0
11	0.0	0.0	0.0	3.849756117949928E-4	0.0
12	0.0	5.499651597071326E-5	0.0	4.3997212776570606E-4	0.0
13	0.0	5.499651597071326E-5	0.0	4.949686437364193E-4	0.0
14	0.0	5.499651597071326E-5	0.0	4.949686437364193E-4	0.0
15	0.0	5.499651597071326E-5	0.0	7.149547076192723E-4	0.0
16	0.0	5.499651597071326E-5	0.0	0.001099930319414265	0.0
17	0.0	5.499651597071326E-5	0.0	0.0013749128992678315	0.0
18	0.0	5.499651597071326E-5	0.0	0.0016498954791213977	0.0
19	0.0	5.499651597071326E-5	0.0	0.0018148850270335373	0.0
20	0.0	5.499651597071326E-5	0.0	0.0019798745749456774	0.0
21	0.0	5.499651597071326E-5	0.0	0.0024748432186820963	0.0
22	0.0	5.499651597071326E-5	0.0	0.0037397630860085012	0.0
23	0.0	5.499651597071326E-5	0.0	0.006819567980368444	0.0
24	0.0	5.499651597071326E-5	0.0	0.012649198673264049	0.0
25	0.0	5.499651597071326E-5	0.0	0.01820384678630609	0.0
26	0.0	5.499651597071326E-5	0.0	0.029093156948507313	0.0
27	0.0	5.499651597071326E-5	0.0	0.08887436980867262	0.0
28	0.0	5.499651597071326E-5	0.0	0.15025048163198862	0.0
29	0.0	5.499651597071326E-5	0.0	0.22053602904256014	0.0
30	0.0	5.499651597071326E-5	0.0	0.3295941202124845	0.0
31	0.0	5.499651597071326E-5	0.0	0.5238418146210437	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3460	0.0	23.0448	1
GGACCGT	90	4.4482465E-5	16.444445	6
TACGTTA	150	4.6748028E-9	16.033333	19
CGCATAC	85	5.366283E-4	15.235294	37
ATAGTCG	85	5.366283E-4	15.235294	20
GTATCAA	5310	0.0	14.946328	2
TAACGTG	75	0.004105627	14.8	5
GGCTAGA	175	2.240995E-9	14.8	1
ATTACGT	165	1.6467311E-8	14.575757	17
CGACGAG	115	2.2111315E-5	14.478261	24
CGAACGT	90	8.2772796E-4	14.388888	4
TAGAACA	180	3.3342076E-9	14.388888	4
GACGGTA	210	1.364242E-10	14.095239	8
ACGTTTA	185	4.9021764E-9	14.0	26
ATTCGTC	135	6.5715267E-6	13.703704	17
TACGACG	220	2.8376235E-10	13.454545	5
GTACTAG	110	2.4575918E-4	13.454545	1
GTATTAG	290	0.0	13.396551	1
TAACGCT	180	5.1632014E-8	13.361111	32
CAAGACG	360	0.0	13.361111	4
>>END_MODULE