FastQCFastQC Report
Wed 25 May 2016
SRR2088397_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088397_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2820027
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT295711.0486069814225183No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT289151.0253447927980832No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT254870.9037856729740531No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG111740.3962373409899976No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98420.3490037506733092No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT61890.21946598383632498No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA61730.21889861338207045No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT59780.21198378597084352No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA50540.17921814223764526No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA50220.17808340132913622No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT47240.16751612661864587No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT46520.16496295957450052No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG42850.15194889978003756No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA38500.1365235155549929No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT36450.12925408160985694No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT34600.12269386073253909No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA33800.11985700846126651No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA33170.11762298729763934No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67600.028.2152351
CGCGGAT500.007037357418.49999814
TTGCGCG1258.592906E-917.7618
GTATCAA116650.016.4303472
GTATTGG7350.016.1088451
ATTGGAC6500.015.6538463
TTGGACC11300.014.8982314
CGACGCA750.00410637314.819
CGGGATA1904.5656634E-1014.60526424
GGACCCT11600.014.1939656
AGAGTGT3400.014.1470596
ATTCGAC1051.6569787E-414.09523821
TGGACCC11500.013.9956525
CGTGGAT5150.013.6504853
TCTCGCG1102.458345E-413.45454515
GTCTATA2102.044544E-913.2142851
CGTCTGT6750.013.15555634
ACGAACG1703.7366954E-713.05882415
TATACCG3000.012.955
ACGTTTA2152.904926E-912.90697726
Produced by FastQC (version 0.11.5)