##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088397_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2820027
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.315766834856547	31.0	31.0	34.0	30.0	34.0
2	31.546094416826506	31.0	31.0	34.0	30.0	34.0
3	31.631741823748495	31.0	31.0	34.0	30.0	34.0
4	35.42721718621843	37.0	35.0	37.0	33.0	37.0
5	35.23711616945511	37.0	35.0	37.0	33.0	37.0
6	35.27394205800157	37.0	35.0	37.0	32.0	37.0
7	35.155856663783716	37.0	35.0	37.0	32.0	37.0
8	35.17076645010846	37.0	35.0	37.0	32.0	37.0
9	36.6665833341312	39.0	35.0	39.0	32.0	39.0
10	36.53326333400354	39.0	35.0	39.0	32.0	39.0
11	36.6537770737656	39.0	35.0	39.0	32.0	39.0
12	36.5166060466797	38.0	35.0	39.0	32.0	39.0
13	36.55927159562657	39.0	35.0	39.0	32.0	39.0
14	37.558195364796156	39.0	36.0	41.0	32.0	41.0
15	37.551363869920394	39.0	36.0	41.0	32.0	41.0
16	37.51623370981909	39.0	36.0	41.0	32.0	41.0
17	37.55919251836951	39.0	36.0	41.0	32.0	41.0
18	37.53702109944337	39.0	36.0	41.0	32.0	41.0
19	37.604684281391634	39.0	36.0	41.0	32.0	41.0
20	37.56722967546055	39.0	36.0	41.0	32.0	41.0
21	37.504250136612164	39.0	36.0	41.0	32.0	41.0
22	37.39923128395579	39.0	36.0	41.0	32.0	41.0
23	37.305770831272184	39.0	36.0	41.0	31.0	41.0
24	37.238549134458644	39.0	36.0	41.0	31.0	41.0
25	37.18170712549915	39.0	36.0	41.0	31.0	41.0
26	36.94622214610002	39.0	36.0	40.0	31.0	41.0
27	36.78944846981962	39.0	35.0	40.0	30.0	41.0
28	36.69347208377792	39.0	35.0	40.0	30.0	41.0
29	36.553070945774635	38.0	35.0	40.0	30.0	41.0
30	36.37270033230178	38.0	35.0	40.0	30.0	41.0
31	36.140805744058476	38.0	35.0	40.0	30.0	41.0
32	35.99153908810093	38.0	35.0	40.0	29.0	41.0
33	36.0588625569897	38.0	35.0	40.0	30.0	41.0
34	36.01647962945036	38.0	35.0	40.0	30.0	41.0
35	35.95913727067152	38.0	35.0	40.0	29.0	41.0
36	35.88194793879633	38.0	35.0	40.0	29.0	41.0
37	35.76944688827447	38.0	35.0	40.0	28.0	41.0
38	35.64505623527718	38.0	34.0	40.0	27.0	41.0
39	35.59236347737096	38.0	34.0	40.0	27.0	41.0
40	35.433782016980686	38.0	34.0	40.0	26.0	41.0
41	35.304843180579475	38.0	34.0	40.0	25.0	41.0
42	35.16675053111194	38.0	34.0	40.0	25.0	41.0
43	34.82036129441314	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	1.0
15	8.0
16	19.0
17	66.0
18	166.0
19	365.0
20	878.0
21	1769.0
22	3310.0
23	5784.0
24	9452.0
25	14975.0
26	22989.0
27	33432.0
28	46775.0
29	63469.0
30	81904.0
31	101032.0
32	124450.0
33	151399.0
34	185401.0
35	225357.0
36	283954.0
37	378157.0
38	497679.0
39	587232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.30854066290854	21.95291037993608	13.342602748129718	23.39594620902566
2	18.050784620147255	23.036233341028296	35.59987191611995	23.3131101227045
3	19.749208074958148	25.764859698151827	31.712426866834964	22.77350536005506
4	13.23685907971803	16.29693616408637	35.69940997018823	34.76679478600737
5	12.26438612112579	39.50504729210039	34.46147856031166	13.769088026462159
6	31.62395253662465	36.703903898792454	15.884245079922993	15.787898484659898
7	25.41291271324707	34.17655221031572	22.030462828901992	18.380072247535217
8	27.29736275574666	33.57896927937215	20.50827882144391	18.61538914343728
9	25.87461042039668	13.742067008578287	22.740881558935428	37.6424410120896
10	16.227007755599505	27.657820297465236	34.07151775497185	22.04365419196341
11	32.44383830367582	22.253297574810453	24.28246254379834	21.020401577715393
12	20.491470471736616	27.33463190246051	31.540442697889066	20.63345492791381
13	30.457722567904494	21.123343854509194	27.67714635356328	20.74178722402303
14	21.18171208999063	22.326523824062676	28.240793439211753	28.25097064673494
15	23.357081332909225	29.048126134962537	25.923510661422743	21.671281870705496
16	22.27624061755437	28.03065360721724	26.865168312218284	22.827937463010105
17	20.373421956598285	27.271618321384867	27.86820835403349	24.486751367983356
18	21.033947547310717	26.4653849058892	31.055092734927715	21.44557481187237
19	20.820722638471192	25.527911612193783	32.2075639701322	21.443801779202822
20	23.753460516512785	24.750578629211706	31.131191297104603	20.364769557170906
21	23.370627302504552	25.362203978898073	30.23754737100035	21.02962134759703
22	22.31365160688178	25.26982897681476	30.833428190581152	21.583091225722306
23	21.503588440819893	26.388364366724147	31.372962031923805	20.73508516053215
24	20.539377814467734	26.119643535327853	30.45839632031892	22.882582329885494
25	21.55234683923239	26.476696854320895	31.11395741955662	20.85699888689009
26	22.2168440231246	25.98638239988482	30.22456877185928	21.5722048051313
27	21.437277018978897	26.8331473422063	30.027939448806695	21.70163619000811
28	21.210612522504217	26.569249159671166	30.194817283664303	22.02532103416031
29	20.384698444376596	26.66045396019258	30.932788941382476	22.022058654048347
30	20.25157915154713	26.937437123828957	31.925864539594834	20.88511918502908
31	21.13993944029614	26.505774590101446	31.074135105798632	21.280150863803787
32	21.678444922690456	26.67009925791491	29.968507393723538	21.682948425671103
33	22.223723389882437	26.462264368390798	29.724396255780526	21.589615985946235
34	21.271888531563704	28.050582494422926	29.789892082593532	20.887636891419834
35	20.384840286990162	28.586215663892578	30.096591273771494	20.932352775345766
36	20.934090347361924	26.51854042532217	31.09668808135525	21.450681145960658
37	21.0308979311191	26.418505922106423	30.631302466252986	21.9192936805215
38	21.085188191460578	26.210564650622135	31.060872821430436	21.643374336486847
39	21.72238067224179	25.834788106638694	31.123425414011997	21.31940580710752
40	20.534165098419273	26.125955531631433	30.8461231044951	22.493756265454195
41	20.99096923540094	25.77514328763519	30.78495347739578	22.448933999568087
42	19.37715489958075	26.90825300608824	31.101156123682504	22.613435970648506
43	19.772292960315628	26.529887834407262	30.858924400369215	22.8388948049079
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	280.0
1	352.5
2	425.0
3	1362.0
4	2299.0
5	2299.0
6	3622.5
7	4946.0
8	5325.5
9	5705.0
10	8620.0
11	11535.0
12	11535.0
13	20036.5
14	28538.0
15	45584.5
16	62631.0
17	61709.5
18	60788.0
19	60788.0
20	65386.0
21	69984.0
22	59076.5
23	48169.0
24	53433.0
25	58697.0
26	58697.0
27	65462.5
28	72228.0
29	76856.5
30	81485.0
31	88614.0
32	95743.0
33	95743.0
34	104416.5
35	113090.0
36	121594.0
37	130098.0
38	135679.5
39	141261.0
40	141261.0
41	147467.5
42	153674.0
43	167398.0
44	181122.0
45	210225.5
46	239329.0
47	239329.0
48	243124.0
49	246919.0
50	228236.0
51	209553.0
52	199638.5
53	189724.0
54	189724.0
55	172026.0
56	154328.0
57	136226.0
58	118124.0
59	106808.0
60	95492.0
61	95492.0
62	84390.5
63	73289.0
64	64691.0
65	56093.0
66	47631.0
67	39169.0
68	39169.0
69	33383.0
70	27597.0
71	23666.5
72	19736.0
73	15895.0
74	12054.0
75	12054.0
76	9434.0
77	6814.0
78	5423.0
79	4032.0
80	3238.0
81	2444.0
82	2444.0
83	1846.0
84	1248.0
85	959.5
86	671.0
87	534.5
88	398.0
89	398.0
90	316.0
91	234.0
92	144.5
93	55.0
94	36.5
95	18.0
96	18.0
97	12.5
98	7.0
99	4.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2820027.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.71140420519224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.54008250604153	34.81871923428085
2	13.64948798723478	12.751735011312793
3	4.934442640760639	6.914842341597191
4	2.282980896818115	4.26564973856013
5	1.2166415127539936	2.8415516737534166
6	0.726932958172582	2.0373635543565345
7	0.49073181779586433	1.6045940608187956
8	0.32726661564708437	1.2229666533084997
9	0.23941254899664363	1.0064966713179853
>10	1.381462671443035	12.022755571806222
>50	0.12787565715329874	4.1523192189770946
>100	0.07301848634526699	6.39959898186765
>500	0.004337251556870957	1.3225101241520236
>1k	0.004565527954581081	3.897189382986213
>5k	4.56552795458108E-4	1.35983101294438
>10k+	3.0436853030540534E-4	3.3818767679601374
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29571	1.0486069814225183	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	28915	1.0253447927980832	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	25487	0.9037856729740531	No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11174	0.3962373409899976	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9842	0.3490037506733092	No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT	6189	0.21946598383632498	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6173	0.21889861338207045	No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT	5978	0.21198378597084352	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5054	0.17921814223764526	No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA	5022	0.17808340132913622	No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT	4724	0.16751612661864587	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT	4652	0.16496295957450052	No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG	4285	0.15194889978003756	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3850	0.1365235155549929	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3645	0.12925408160985694	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT	3460	0.12269386073253909	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3380	0.11985700846126651	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3317	0.11762298729763934	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.546065339090725E-5	0.0	0.0	0.0	0.0
7	3.546065339090725E-5	0.0	0.0	0.0	0.0
8	7.09213067818145E-5	0.0	0.0	0.0	0.0
9	7.09213067818145E-5	0.0	0.0	3.546065339090725E-5	0.0
10	7.09213067818145E-5	0.0	0.0	3.546065339090725E-5	0.0
11	7.09213067818145E-5	0.0	0.0	2.4822457373635076E-4	0.0
12	7.09213067818145E-5	0.0	0.0	3.191458805181652E-4	0.0
13	7.09213067818145E-5	0.0	0.0	3.546065339090725E-4	0.0
14	7.09213067818145E-5	0.0	0.0	4.25527840690887E-4	0.0
15	7.09213067818145E-5	0.0	0.0	6.028311076454232E-4	0.0
16	7.09213067818145E-5	0.0	0.0	8.865163347726812E-4	0.0
17	7.09213067818145E-5	0.0	0.0	0.0014893474424181046	0.0
18	7.09213067818145E-5	0.0	0.0	0.001843953976327177	0.0
19	7.09213067818145E-5	0.0	0.0	0.0021630998568453424	0.0
20	7.09213067818145E-5	0.0	0.0	0.0029077735780543945	0.0
21	7.09213067818145E-5	0.0	0.0	0.004113435793345241	0.0
22	7.09213067818145E-5	0.0	0.0	0.006489299570536027	0.0
23	7.09213067818145E-5	0.0	0.0	0.010921881244399433	0.0
24	7.09213067818145E-5	0.0	0.0	0.019184213484480822	0.0
25	7.09213067818145E-5	0.0	0.0	0.02471607541346235	0.0
26	7.09213067818145E-5	0.0	0.0	0.033262092880671	0.0
27	7.09213067818145E-5	0.0	0.0	0.10159477196494927	0.0
28	7.09213067818145E-5	0.0	0.0	0.17148771979842747	0.0
29	7.09213067818145E-5	0.0	0.0	0.2514869538483142	0.0
30	7.09213067818145E-5	0.0	0.0	0.3899253446864161	0.0
31	7.09213067818145E-5	0.0	0.0	0.6146395052245953	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6760	0.0	28.215235	1
CGCGGAT	50	0.0070373574	18.499998	14
TTGCGCG	125	8.592906E-9	17.76	18
GTATCAA	11665	0.0	16.430347	2
GTATTGG	735	0.0	16.108845	1
ATTGGAC	650	0.0	15.653846	3
TTGGACC	1130	0.0	14.898231	4
CGACGCA	75	0.004106373	14.8	19
CGGGATA	190	4.5656634E-10	14.605264	24
GGACCCT	1160	0.0	14.193965	6
AGAGTGT	340	0.0	14.147059	6
ATTCGAC	105	1.6569787E-4	14.095238	21
TGGACCC	1150	0.0	13.995652	5
CGTGGAT	515	0.0	13.650485	3
TCTCGCG	110	2.458345E-4	13.454545	15
GTCTATA	210	2.044544E-9	13.214285	1
CGTCTGT	675	0.0	13.155556	34
ACGAACG	170	3.7366954E-7	13.058824	15
TATACCG	300	0.0	12.95	5
ACGTTTA	215	2.904926E-9	12.906977	26
>>END_MODULE