FastQCFastQC Report
Wed 25 May 2016
SRR2088396_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088396_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341664
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT338481.0129085389793826No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT317910.9513523801315751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT285810.8552924531012095No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG134760.40327214226205865No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111730.334354381529681No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA75650.2263842205559865No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT73650.22039917837340917No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA72280.21629942447834372No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT71360.21354630507435818No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA60510.1810774512338763No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT58950.176409118331466No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT51210.15324700508489184No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG51180.15315722945215318No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA44460.13304748771869343No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA42400.1268828942706388No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41300.1235911210702213No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT38410.11494273511639709No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA38280.11455370737452958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77550.028.1495821
TATACCG2050.017.1463415
GTATTGG10600.017.1037731
ATTGGAC9850.016.7157363
GTATCAA132200.016.41492
CGCACTA1851.8189894E-1116.029
GGACCCT15350.015.7882736
TAGTACT5750.015.4434794
GTACTAG3000.015.4166661
TTGGACC16750.015.352244
TGCGACG1455.3551048E-815.31034522
CGCGATA1101.4534382E-515.13636414
TGCGGTC1601.0986696E-815.0312512
TGGACCC16900.014.5591735
GCGTTAT2750.014.1272741
GACGTAT1458.923853E-714.03448328
GACCCTC17000.013.9294127
ATAACGC2405.456968E-1213.8750013
TACGTTA1601.7864477E-713.87519
ACGGACC3350.013.8059698
Produced by FastQC (version 0.11.5)