FastQCFastQC Report
Wed 25 May 2016
SRR2088395_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088395_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3156459
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT401281.271297995633715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT399591.26594389472507No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT356551.1295885674421877No Hit
GTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG182710.5788448384724781No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134410.42582526812481963No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA107190.33958939431812674No Hit
GGTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACT97490.3088587559667336No Hit
ACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTAGGAAT94480.2993227537566621No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA93530.2963130520624535No Hit
TATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTTA80040.25357528800469137No Hit
GTATCAACGCAGAGTACATGGGCTTGCTACACGATTCAGACTT75780.24007915198645063No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCATGT68830.2180608080130298No Hit
GAGTACATGGGCTTGCTACACGATTCAGACTTAGGAATGTTCG67430.21362545814788028No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA60360.1912269413288752No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA60120.19046659563770668No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA51730.1638861775172749No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50860.16112992438678914No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTT49770.15767668770606558No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA42180.13363075522286208No Hit
AGTCTGAATCGTGTAGCAAGCCCATGTACTCTGCGTTGATACC38350.12149690523463158No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG36790.11655465824203641No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35390.1121193083768869No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG35120.11126391947432233No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT33180.1051177918040437No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA32360.1025199440258847No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32280.10226649546216186No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94300.027.5832441
GTATTGG10000.020.9049991
CGCACTA1351.8189894E-1220.55555529
ATTGGAC10250.019.4926823
ATCACGT1502.5283953E-1017.26666623
TTGGACC18700.016.9171124
CGTCTGT6400.016.187534
GTATCAA161450.016.0994112
TATTGGA14000.015.5928572
TGGACCC19150.015.5535245
GGACCCT19100.015.4973836
AGCCGTC2301.8189894E-1214.47826112
CGCGAAT908.2796626E-414.38888835
TTTTTAC18300.014.3551921
CGGTATA2450.014.34693826
GCGTTAT2353.6379788E-1214.1702121
TATACTG4050.014.1604945
GACCCTC20250.013.9777787
GTATACG2009.858923E-1013.8749991
GCCGTCA2700.013.70370413
Produced by FastQC (version 0.11.5)